9hc4

Cryo-EM structure of P. urativorans 70S ribosome with 2 copies of bS20.

Method: ELECTRON MICROSCOPY D_max: 297.2 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9hc4
沉积日期 deposition_date	2024-11-08
结构标题 title	Cryo-EM structure of P. urativorans 70S ribosome with 2 copies of bS20.
关键词 keywords	Ribosome, ribosome heterogeneity, functionally specialized ribosomes, polysome; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	83.61 Å
回转半径 Rg (电子) rg_electron	84.58 Å
零角强度 I(0) i0	133191000000.00
分子量 molecular_weight	2027100.0 kDa
排除体积 excluded_volume	2087500 Å³
包络体积 envelope_volume	3679500 Å³
水化壳体积 shell_volume	334720 Å³
包络直径 envelope_diameter	277.1 Å
壳层 Rg shell_rg	97.80 Å
包络 Rg envelope_rg	82.53 Å
形状 Rg shape_rg	84.65 Å
总 Rg total_rg	84.52 Å
总原子数 total_atoms	136430
残基数 n_residues	9929
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	297.2 Å
Rg (实空间) rg_real	86.38 Å
Rg 误差 (实空间) rg_real_error	1.57 Å
I(0) (实空间) i0_real	1.3270e+11
I(0) 误差 (实空间) i0_real_error	2.6280e+09
Rg (倒空间) rg_reciprocal	84.88 Å
I(0) (倒空间) i0_reciprocal	133700000000.0000
解质量估计 total_estimate	0.9064
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	109.5 Å
偏度 Skewness skewness	0.408
峰度 Kurtosis kurtosis	0.109
角度范围 angular_range	— – 0.0950 Å⁻¹
当前正则化参数 α current_alpha	1.1070
最高正则化参数 α highest_alpha	6895000000.0000
实空间数据点数 n_real_points	20
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.773; Stabil: 0.898; Sysdev: 1.000; Positv: 1.000; Valcen: 0.970; Smooth: 0.815

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (52)

Entity 1 polymer

描述 description	Small ribosomal subunit protein uS9
分子量 formula_weight	14278.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F
原子数 atom_count	136434

MERNYGTGRRKTSTARVFLAKGTGSIVVNGKPLDEYFSRETSRMVVRQPLELLDSPTAYDLYITVKGGGISGQAGAIRHGITRALIELDDTNKPALKAAGFVTRDSRQVERKKLGLRKARKRPQFSKR

Entity 2 polymer

描述 description	Large ribosomal subunit protein bL9
分子量 formula_weight	18195.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C1

MQIILLQRIVNLGKLGETVDVKPGYGRNFLIPLGKALPATAANIEKFEARRAELEAEEKKELDAAQERADALTDVNVIMRAKSGDEGKLFGSIGTRDIAEALTNSGLEVDRAEIKLPEGALRQVGEYNVDIQLHHDVTATILVTILSEDGDDQVEEDDNEGDYSEE

Entity 3 polymer

描述 description	Small ribosomal subunit protein bS18
分子量 formula_weight	8973.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R3

MARFYRRRKFCRFTAEGITHIDYKDVELLKQYISENGKIVPSRITGTSTKYQRQLATAIKQARYLSLLPYTDNHQG

Entity 4 polymer

描述 description	Small ribosomal subunit protein uS2
分子量 formula_weight	29465.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A4

MATKNPTKIEMRDLLQAGAHFGHQTRFWNPKMGPYIFGARNKIHIINLEHTVKAFNEALNYVNGLASKKNKVLFVGTKRAASGIIAEQATRAGMPYVDHRWLGGMLTNWKTLRQSINRLKELEKQAEDGTFAKLTKREALERTRDMQKLERSLGGIKDMGGLPDAIFVVDVDHEAIAIKEAKNLGIPVIGIVDTNSNPDNVDYIIPANDDAIRAVSLYVTAMADAIIAGKEYAQTQASGNAADAEQEVEQAATAEEAADVATPVEAKAE

Entity 5 polymer

描述 description	Small ribosomal subunit protein uS5
分子量 formula_weight	18192.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E5

MARNDKNDKNEQTDGLVERLVTVDRVAKVVKGGRIFSFTALTVVGDGNGRVGFGRGKAREVPAAIQKALEAAKRNMITVELNDATLHHPIKARHGASKVYMQPASEGTGVIAGGAMRAVLEVAGVKDVLTKCYGSTNTANVVRATFNGLRDMSTPEKMAAKRGKSVDEILG

Entity 6 polymer

描述 description	Small ribosomal subunit protein uS12
分子量 formula_weight	13753.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L6

MATTNQLIRKGRKTIKEKSKVPALQACPQRRGVCTRVYTTTPKKPNSAMRKVCRVRLTSGYEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGALDCAGVKDRKQGRSKYGAKKPKA

Entity 7 polymer

描述 description	Large ribosomal subunit protein uL5
分子量 formula_weight	19841.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F7

MARLKALYNNELKQKIKEELGLDNVMQVPKITKITLNMGVGGASQDKKLLEGAVADMTAIAGQKPVVTKARKSIAGFKIREEWPIGCKVTLRGEQMYEFLDRLIAVAIPRVRDFRGFSPKAFDGRGNYSLGIKEQIVFPEVDFDKIDRIRGMDVTITTSAATDDEGRALLKAFGFPFK

Entity 8 polymer

描述 description	Large ribosomal subunit protein uL4
分子量 formula_weight	21971.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E9

MDLKTVTGAAVELSDTAFGREFNEALVHQVVTAYLAGGRQGTRAQKTRAEVSGGGIKPWRQKGTGRARAGSIRSPIWRGGGRAFAAKPQDWSQKVNRKMYRGAMQCILAELIRQERLILVEELTISAPKTKELIAKLSELNAPRALIITKEVDENLYLAARNIPHVNVLDTSEVDPVSLIAFDKVIMSVEAAKQFEEALA

Entity 9 polymer

描述 description	Large ribosomal subunit protein bL32
分子量 formula_weight	7169.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	aA

MAVQKSRKSRSRRDMRRSHHHMTVAELSVDATTGEKHRRHHMTKDGFYRGRQLFKVSQEA

Entity 10 polymer

描述 description	Large ribosomal subunit protein bL17
分子量 formula_weight	13635.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	MB

MRHRKSGVKLGRTSSHRKAMFQNMTNSLFEHELIKTTLPKAKELRRVAEPLITMAKEDSVANRRLAFSRMRSKTMVGKLFGTLGPRYQNRPGGYLRIVKCGNRDGDNAPMAYVELVDRD

Entity 11 polymer

描述 description	Large ribosomal subunit protein bL25
分子量 formula_weight	23887.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	UC

MSINHFALNAVDRSAELQGKGASRRLRKQNLVPAIIYGGNEEPASISIKINELVKALEFEAFFSHILTITVDGVEEQAVIKALQRHPAKGFPMHADFQRIVKGQKINMNVPLHFTGAEDAPGTKKSGILSTVVTDVEIVCLPSQLPEYLAVDVSGMEIGDLFRLSDIKLPEGVILFDLDMEDAHDRTIVNMQAPTVEEVDTATVDASEVPATAQTKDAE

Entity 12 polymer

描述 description	Large ribosomal subunit protein bL28
分子量 formula_weight	9168.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	WD

MSRVCQVTGKRPMVGNNVSHANNKTRRRFLPNLHHHRFWVESENRFVRLRISTKGMRIIDKLGIDKVLVDLRAQGQKV

Entity 13 polymer

描述 description	Large ribosomal subunit protein uL29
分子量 formula_weight	7519.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	XE

MKISELRDKSLEELTQLLDEKQLDAFRIRMAKATGQLGNTHEVRVNRRAIAQLQTLINEKQRGDS

Entity 14 polymer

描述 description	Large ribosomal subunit protein uL22
分子量 formula_weight	11933.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	RF

MEVTAKLRGAAISAQKVRLVADEVRGKSIERALDILTYSNKKGAVFVKKCLNSAIANAEHNHGLDIDGLKVATIYVDEGITLKRILPRAKGRADRISKRTCHITIKVGE

Entity 15 polymer

描述 description	Small ribosomal subunit protein bS6
分子量 formula_weight	15839.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	FG

MRHYEVVLLVHPDQSDQVVGMVERYIKLVQDNNGIIHRLEDWGRRQLAYPINKIHKAHYVLFNIETNGETLAELEELFRYNDAIIRSLVMRRDDAITEESQLAKNADEKRARKATTRRPDSRENDNDDNDHSED

Entity 16 polymer

描述 description	Large ribosomal subunit protein bL27
分子量 formula_weight	9119.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	VH

MAHKKAAGSSRNGRDSNPKMLGVKIFGGQAIVAGNIIVRQRGTEFHAGAGVGMGRDHTLFALNDGVVKFATKGKFNRRYVMVEEA

Entity 17 polymer

描述 description	Large ribosomal subunit protein uL24
分子量 formula_weight	11465.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	TI

MSKLRKGDTVIVIAGKDKGKQGTVQAVKNDRIKVEGINIVTKHQKPNQATGVEGGILKQEAFLHISNVAILNAQTQKADRITYQFGEDGKKQRVYRSNGEVVATA

Entity 18 polymer

描述 description	Large ribosomal subunit protein bL31B
分子量 formula_weight	10708.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	fJ

MRKDIHPDYHDVLFHDTNADVFFLTRSTVKTKTTREYEGSEYPYYPLDISSASHPFYTGEQRKTSTEGRVASFNKRFGAFGGRNKKADTDSAE

Entity 19 polymer

描述 description	Small ribosomal subunit protein uS14
分子量 formula_weight	11777.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	HK

MAKKSMINRELKREKEVAKYADKRTKLKETISDMNASDEVRMDAMLELQALPRNSSPVRLRNRCAITGRPHGYFRKFGLSRNMLRERVMQGDVPGVRKASW

Entity 20 polymer

描述 description	Small ribosomal subunit protein uS15
分子量 formula_weight	10218.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	OL

MLTNSDREQIIAQYQRGENDTGSPEVQVALLSARINDLQNHFKAHKADHHSRRGLIRMVNTRRKLLDYLKGKDLGRYTTLISQLGLRR

Entity 21 polymer

描述 description	Small ribosomal subunit protein uS13
分子量 formula_weight	13244.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	MM

MARIAGVNIPDNKHAVISLTYIFGVGRTTAQKILEAVGIAPTTKVSQLDDIQLDAIRAQVANYMTEGDLRREVSMNIKRLVDLGCYRGIRHRRNLPVRGQNTKNNARTRKGPTRPLKR

Entity 22 polymer

描述 description	Large ribosomal subunit protein bL20
分子量 formula_weight	13540.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	PO

MARVKRGVQANRRHKKILKRAKGYYGARSRVYRVAVQAVTKAGQYAYRDRRNKKRTFRRLWIARINAGARLNGLSYSRFINGMKKANITIDRRVLADIAMHDAATFGALVEKAKAELA

Entity 23 polymer

描述 description	Small ribosomal subunit protein uS19
分子量 formula_weight	10336.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	SP

MPRSLKKGPFIDAHLFAKVENALETNSRKPIKTWSRRSMILPQMVGLTLSVHNGRTHVPVIVSEQMVGHKLGEFAPTRTYRGHGIDRKAKR

Entity 24 polymer

描述 description	Small ribosomal subunit protein uS8
分子量 formula_weight	14086.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	BQ

MSMQDTVGDMLTRIRNAQMANKVSVAMPSSKLRKSIADLLVSEGYVASAVVNAEENNKATLSIELKYFEGKAVIETIQRFSRPGLRQHRGKDAIPTVKQGMGVAIVSTSQGIMSDRAARAAGIGGEVVAFVA

Entity 25 polymer

描述 description	Large ribosomal subunit protein uL6
分子量 formula_weight	19536.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	GR

MSRVAKAPVTLPNGVSVTLNDRQVEVKGKNGNLSLRLHDLVELKQEDDVVTLSPTVDSKEAMMHTGTIRALLNNYVIGVTEGFERRLQLIGVGYRAQATGNKVTLNVGYSHPVEYTLPEGVSAETPTQTEIVLKSNDKQQLGQAAAKIRGFRPPEPYKGKGIRYSDEHVIRKEAKKK

Entity 26 polymer

描述 description	Small ribosomal subunit protein uS7
分子量 formula_weight	17760.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	GS

MPRRRVVATREILPDPKFGSQTIAKFINHVMSHGKKSTAERIVYGALETVAQKRNIEDPVAFFEDVLENVRPMVEVKARRVGGATYQVPMEVRPSRRTALAMRWLAEAAAKRSEKSMALRLAGELSDASEGKGNAMKKRDEVHRMADANKAFSHYRF

Entity 27 polymer

描述 description	Small ribosomal subunit protein uS3
分子量 formula_weight	27120.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	CT

MGQKVHPIGIRLGVVKKHNANWYASPKQYSEYLINDIQVREYLRKKLDSAMISNIMIERPTGAAKITIATARPGIVIGKKGEDIERLQKELTKMMGVPAQVNIEEITSPDLDARLVADGIGSQLERRVMFRRAMKRAVQNSMRSGAKGIKVELSGRLGGAEIARTEWYREGRVPLHTLRADIDYASVRAETTYGTIGVKVWIFRGEILDGMNSVYNPVKEEQTRAPKRRGRGGNRRNSDRG

Entity 28 polymer

描述 description	Small ribosomal subunit protein uS11
分子量 formula_weight	13676.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	KU

MAKDTRSRKKVTRRSVSEGIAHIHASFNNTIVTITDRQGNALAWATSGGQGFRGSRKSTPFAAQVAAEVAGKAAQEYGVKNIDVLVKGPGPGRESAVRALGALGYKVNSISDVTPIPHNGCRAPKKRRV

Entity 29 polymer

描述 description	Small ribosomal subunit protein bS21
分子量 formula_weight	8548.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	NV

MPAVKVKENEPVDIAIRRFKRACEKAGVLSDVRKREFYEKPTQVRKRKKAAAVKRYKKKLQRETIRTTRMY

Entity 30 polymer

描述 description	Large ribosomal subunit protein uL30
分子量 formula_weight	6615.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	YW

MKTMKVTQLKSGAHRLKSHKASLKGLGLRRINHSVVVEDTPSTRGMVNRVNYLVKVEEA

Entity 31 polymer

描述 description	Large ribosomal subunit protein uL13
分子量 formula_weight	15802.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	IX

MKTLSAKPAEVTHDWFVVDADGKTLGRLATQIASRLRGKHKTNYTPHVDTGDFIVVINAEKIAVTGKKAQDKKYYRHSGYPGGIKETNFTKLIAHKPEDVLHKAVKGMLPKGPLGYAMIKKLKLYAGTDHPHEAQQPKELDI

Entity 32 polymer

描述 description	Small ribosomal subunit protein uS10
分子量 formula_weight	11635.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	JY

MANQRIRIRLKSFDHRLIDQSAQEIVDTAKRTGAQVCGPVPLPTRIERFNVLTSPHVNKDARDQYEIRTHKRMVDIVQPTDKTVDALMKLDLAAGVDVQIALG

Entity 33 polymer

描述 description	Small ribosomal subunit protein uS17
分子量 formula_weight	10363.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	QZ

MSDNNQATTNASVLTGRVVSDKMDKSITVLIERLVRHPLYGKQLRRSTKIKAHDENNVCQQGDLVRIKETRPISKTKAWTLVDVVEKVEKI

Entity 34 polymer

描述 description	Large ribosomal subunit protein bL34
分子量 formula_weight	5292.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ba

MKRTFQPSVIKRKRTHGFRARMATKKGRQVLARRRAKGRHRLTV

Entity 35 polymer

描述 description	Large ribosomal subunit protein bL21
分子量 formula_weight	11773.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Qb

MYAVIKTGGKQHRVVVDELLKVELLKAEQGETITFNDVLMVVDGETVKIGQPVVDGASVEVEVIEHGRGEKIRIVKHNRRKHYHKEQGHRQWYTLLKIKAINA

Entity 36 polymer

描述 description	Large ribosomal subunit protein uL18
分子量 formula_weight	12550.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Nc

MFDKKAARLRRAKKTRAHIRFLGVYRLTVNRTPKHIYAQIISPNGGEVIAQASTLDGSLRSGATGNADAATSVGQLIAERAKAAGITKVAFDRSGFKYHGRVKALAEAARENGLEF

Entity 37 polymer

描述 description	Large ribosomal subunit protein uL15
分子量 formula_weight	15448.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Kd

MGLRLNELSPGVGAKKTAQRRGRGIGSGLGKTGGRGVKGQKSRSGSSIRAGFEGGQTPLYRRLPKFGFTSKMAMTTAEVRLSELNKVEGDVISVATLKTANIIRHDMKRARIILSGDVTKAYTFQGVKVTKGAKQAIEAAGGSIEE

Entity 38 polymer

描述 description	Large ribosomal subunit protein uL14
分子量 formula_weight	13417.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Je

MIQVESMLEVADNSGARRVQCIKVLGGSHRRYASVGDIIKVTVKEAIPRGRVKKGDVMNAVVVRTKKGVRRPDGSVLRFDDNAAVLLNQNKAPIATRIFGPVTRELRGDQFMKIVSLAPEVL

Entity 39 polymer

描述 description	Large ribosomal subunit protein uL3
分子量 formula_weight	22236.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Af

MAIGLVGKKCGMTRVFTEAGASIPVTVVEISANRITQVKNTDVDGYQAIQVTTGTRRDSRVTAAQKGHFAKAGVAAGRGVWEFRANDSDLEGREIGGEILADLFEQGQMVDVTGNSKGKGFQGGVKRHNFSMQDATHGNSVSHRAIGSTGQNQSPGKVFKGKKMPGQMGNKRVTVQGLEVISVDVEKGLLVIKGAIPGATGGDVIVRPSVKA

Entity 40 polymer

描述 description	Large ribosomal subunit protein uL16
分子量 formula_weight	15626.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lg

MLQPKRTKFRKMHKGRNTGLAHRGSTVAFGQFGLKSLTRGRMTARQIEAARRTITRKIKRGGKIWIRVFPDKPISNKPLEVRMGKGKGPVEYWVCEIKPGKMLYELEGISEELAREALTLAAAKLPFKTTIVKRTIM

Entity 41 polymer

描述 description	Large ribosomal subunit protein bL35
分子量 formula_weight	7594.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	dh

MKVKMKTKSGAAKRFKRTANGFKRKQAFKSHILTKKSSKRIRQLRGLKMVDKSDEAAVRRMCPYI

Entity 42 polymer

描述 description	Large ribosomal subunit protein bL19
分子量 formula_weight	14598.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Oi

MSNKHLLVQAVENAQIIERPSFGPGDTVVVQVKVREGERERLQAFEGIVIAKRNRGLNSAFTVRKISSGVGVERAFQLHSPIIESIEVKRRGAVRRAKLYYLRERSGKSARIREKLAPRAPKVVKPKADA

Entity 43 polymer

描述 description	Small ribosomal subunit protein bS16
分子量 formula_weight	9955.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Pj

MVVIRLARGGAKKRPFYQVVVADQRRARDGRYIENIGFFNPLAKESEEAVRLNMEAYHAWIAKGAQPSDRVASLVRAYNKAATTTEATA

Entity 44 polymer

描述 description	Large ribosomal subunit protein bL33
分子量 formula_weight	6053.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	bk

MRDKIKLVSTAGTGYYYTTTKNKRTMPGKMEMKKFDPKIRQHVLFKEAKIK

Entity 45 polymer

描述 description	Large ribosomal subunit protein uL2
分子量 formula_weight	30263.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Cl

MPIVKAKPTSPGRRFVEKVVHPHLYKGRPFAALLESKSKTGGRNNNGRITTRHIGGGHKQHYRIIDFKRTKDNIPATVERIEYDPNRTAHIALLKYADGERRYIIAAKKQAVGDTVMSGEASPIRPGNCLPLKNIPLGTVIHNIELKIGKGAQMARAAGASVQLLGRDGIYAILRLRSGETRRVHVNCRAVIGEVSNTENNLKSLGKAGASRWRGIRPSVRGVAMNPVDHPHGGGEGRNKGRHPTSPWGQKSKGLKTRHNKRTDSMIIRRAKKK

Entity 46 polymer

描述 description	Small ribosomal subunit protein uS4
分子量 formula_weight	24279.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Dm

MARYIGPKLKLSRREGTDLGLKSGVKPYDVKTKKAARPPGQHGVSRNKTSEYALQLREKQKVKRIYGVLERQFANYYKEAARKRGATGENLLAMLESRLDNVVYRMGFGSTRAEARQLVSHRTVMVKKAGRDEFVRVNIPSIQLQDGDVIAIQEKSREQLRIKNAIELATQRGIPEWLDVDHSKLQGTFKKAPDRIDLPAEINESLIVELYSK

Entity 47 polymer

描述 description	Large ribosomal subunit protein uL23
分子量 formula_weight	12816.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Sn

MNNARLYQVLRGPVFSEKSQMLGDSLGVQVFKIDSKATKLEVKKAVELMFEGVEVLKVNTLNVKGKTKRFGKNIGRRNDYKKAYVTLKAGHDVQMADAGEEVANTTASTSETANNE

Entity 48 polymer

描述 description	Small ribosomal subunit protein bS20
分子量 formula_weight	9980.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	To,A

MANTAQARKRARQNTKRRQNSASQRSMVRTYLKRVDAAIAAKDYDAATEAYKKAIPVLDRMADKGIIHKNKAARRKSRLNKTIKGLQA

Entity 49 polymer

描述 description	Large ribosomal subunit protein bL36
分子量 formula_weight	4330.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	ep

MKVQASVKKICGSCKVVRRKGRVHIICTAEPRHKQRQG

Entity 50 polymer

描述 description	5S rRNA
分子量 formula_weight	36948.9 kDa
突变 mutation	insertion of 85, C89A
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	D8

GCUGACGACCAUAGCACGAUGGCACCACCUGAUCCCUUCCCGAACUCAGAAGUGAAACAUCGUCGCGCCAAUGGUAGUGUGGUUUCGACCAUGUGAGAGUAGGUCAUCGUCAGCU

Entity 51 polymer

描述 description	16S rRNA
分子量 formula_weight	514644.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	iN

GAUGAGCCAAGUUUAGAAGCUUCUUUAAAGAGCUUCAUAGCAAGAUUAAACUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCUUAACACAUGCAAGUCGAGCGGUAACAGGAGAAGCUUGCUUCUUGCUGACGAGCGGCGGACGGGUGAGUAAUACUUAGGAAUCUACCUAGUAGUGGGGGAUAGCUCGGGGAAACUCGAAUUAAUACCGCAUACGACCUACGGGAGAAAGGGGGCAACUUGUUGCUCUCGCUAUUAGAUGAGCCUAAGUCGGAUUAGCUAGAUGGUGGGGUAAAGGCCUACCAUGGCGACGAUCUGUAGCUGGUCUGAGAGGAUGAUCAGCCACACCGGGACUGAGACACGGCCCGGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGGACAAUGGGGGCAACCCUGAUCCAGCCAUGCCGCGUGUGUGAAGAAGGCCUUUUGGUUGUAAAGCACUUUAAGCAGUGAAGAAGACUCCGUGGUUAAUACCCACGGACGAUGACAUUAGCUGCAGAAUAAGCACCGGCUAACUCUGUGCCAGCAGCCGCGGUAAUACAGAGGGUGCAAGCGUUAAUCGGAAUUACUGGGCGUAAAGGGAGCGUAGGUGGCUCGAUAAGUCAGAUGUGAAAUCCCCGGGCUUAACCUGGGAACUGGCAUCUGAUACUGUUGAGCUAGAGUAUGUGAGAGGAAGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCGAUGGCGAAGGCAGCCUUCUGGCAUAAUACUGACACUGAGGCUCGAAAGCGUGGGUAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGUCUACUAGUCGUUGGGUCCCCUUGAGGACUUAGUGACGCAGCUAACGCAAUAAGUAGACCGCCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUUGUUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUAUCUAGAAUCCUGCAGAGAUGCGGGAGUGCCUUCGGGAAUUAGAAUACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUGUCCUUAGUUACCAGCGGGUUAAGCCGGGAACUCUAAGGAUACUGCCAGUGACAAACUGGAGGAAGGCGGGGACGACGUCAAGUCAUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGUAGGUACAGAGGGCAGCUACACAGCGAUGUGAUGCGAAUCUCAAAAAGCCUAUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUAGGAAUCGCUAGUAAUCGCGGAUCAGAAUGCCGCGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUUGAUUGCACCAGAAGUGGAUAGCUUAACCUUCGGGAAGGCGUUCACCACGGUGUGGUUGAUGACUGGGGUGAAGUCGUAACAAGGUAGCCGUAGGGGAACCUGCGGCUGGAUCACCUCCUUAU

Entity 52 polymer

描述 description	23S rRNA
分子量 formula_weight	933563.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	Z2

GUUGUAUGGUCAAGUAAUGAAGCGCACAUGGUGGAUGCCUUGGCAGUCAGAGGCGAUGAAAGACGUGACAGCCUGCGAUAAGCUUCGGGGAGGCGGCAAUAUCCUGUGAUCCGGAGAUUUCUGAAUGGGGAAACCCACUUACCAUAAGGUAGGUAUCUUGUACUUGUACAAGAAGCGAACGAGGGGAAGUGAAACAUCUCAGUACCCUUAGGAAAAGACAUCAAAUGAGAUUCCCCAAGUAGCGGCGAGCGAACGGGGAGAAGCCGAUUAAUGCUAAUGUAGAAGAACAGCGUGGGAAAGCUGACCGUAGUAGGUGAUAGUCCUGUAUUCGAAACAUUAGCGUUAACAUAUUAAGUAGGGCGGGACACGAGAAAUCCUGUCUGAAGAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCUGUGAGGGGAGUGAAAUAGAACCUGAAACCGUGUGCGUACAAGCAGUAGGAGCCUCAAUUUAUUGGGGUGACUGCGUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGUUUAGGGGAGCCGUAGGGAAACCGAGUCUUAAUAGGGCGUCUAGUUGCAGGGUAUAGACCCGAAACCGAGUGAUCUAUCCAUGAGCAGGUUGAAAGUGCCGUAACAGGCACCGGAGGACCGAACCCACUGUCGUUGAAAAGCCAGGGGAUGACUUGUGGAUAGGGGUGAAAGGCUAAUCAAACUCGGUGAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGGACGAAUACCAUUGGGGGUAGAGCACUGUUUCGGCUAGGGGGUCAUACCGACUUACCAAACCGAUGCAAACUCCGAAUACCGAUGAGUAAUAUCCGGGAGACACACGGCGGGUGCUAACGUCCGUCGUGGAGAGGGAAACAACCCAGACCGCCAGCUAAGGCCCCAAAUUCCUAGUUAAGUGGGAAACGAGGUGGGAAGGCAUAGACAGCUAGGAGGUUGGCUUAGAAGCAGCCAUCCUUUAAAGAAAGCGUAAUAGCUCACUAGUCGAGUCGGCCUGCGCGGAAGAUGUAACGGGGCUCAAACUAGGAGCCGAAGCUGCGGAUUUGAAACUUGUUUUCAAGUGGUAGGGGAGCGUUGUGUAAGCCUGUGAAGGUGUGUCGUAAGGCAUGCUGGAGGUAUCACAAGAGCGAAUGCUGACGUGAGUAACGAUAAUGCGAGUGAAAAGCUCGCACGCCGGAAGAUCAAGGGUUCCAGUCCAACGUUAAUCGGGGCUGGGUGAGUCGACCCCUAAGGCGAGGCCGAAAGGCGUAGUCGAUGGGAAAUUGGUUAAUAUUCCAAUACUUGUUUAUAGUGCGAUGGAGGGACGGAGAAGGUUAUGUCAGCCUGGCGUUGGUUGUCCAGGUGAAAGGAUGUAGGCUUACAGUUUAGGCAAAUCCGGACUGUAAUAUGGCUGAGAUCUGAUAGCAAGCUGUACUUGUACAGCGAAGUGGCAAAUACCAUGCUUCCAGGAAAAGCUUCUAAGCAAUAGCUAUAAACGAAUCGUACCCUAAACCGACACAGGUGAUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCUGCUGAAGGAACUAGGCAAAAUGGUACCGUAACUUCGGGAGAAGGUACGCUGUUGAUGGUGAUAGGACUUUCUCCUUGAGCUUUCGACAGUCGCAGAUACCAGGCUGCUGCAACUGUUUAUUAAAAACACAGCACUCUGCAAACACGAAAGUGGACGUAUAGGGUGUGAUGCCUGCCCGGUGCUGGAAGGUUAAUUGAUGGGGUUAGCGUAAGCGAAGCUCUUGAUCGAAGCCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCAGGUAAGUUCUGACCUGCACGAAUGGCAUAAUGAUGGCAGCGCUGUCUCCAGCAGAGACUCAGUGAAAUCGAAAUCGCAGUGAAGAUGCUGUGUACCCGCGGCUAGACGGAAAGACCCCGUGAACCUUUACUACAGCUUUACAUUGAACUUUGACCUGACUUGUGCAGGAUAGGUGGGAGGCUUUGAAGCCGAGACGCUAGUCUCGGUGGAGCCAAUCUUGAAAUACCACCCUGGUCAUGUCGGGGUUCUAACUCAGGUAUAACAAUACCGAGGACAAUGUAUGGUGGGUAGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGUACGAAGGUGCGCUCAGAACGGUCGGAAAUCGUUCAUAGAGUAUAAAGGCAAAAGCGCGCUUAACUGCGAGACCCACAAGUCGAGCAGGUACGAAAGUAGGUCUUAGUGAUCCGGUGGUUCUGUAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCUGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCUCAUCCUAGGGCUGAAGCAGGUCCUAAGGGUAUGGCUGUUCGCCAUUUAAAGAGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUACCGUGGGCGUUGGAAAUUUGAGAGGAUCUGCUCCUAGUACGAGAGGACCAGAGUGGACGAACCUCUGGUGUUCGGGUUGUCACGCCAGUGGCAUUGCCCGGUAGCUACGUUCGGAUGGGAUAACCGCUGAAAGCAUCUAAGCGGGAAGCCCACCUCAAGAUUAGAUUUCCCUAAAGAGCCGUUGAAGACUACGACGUUGAUAGGCAGGGUGUGGAAGUGCAGCGAUGCAUGUAGCUAACCUGUACUAAUUGCUCGUUUGGCUUGACCAUACAACACCCAAG

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.20 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	19576

fit	/app/data/saxs_computation/hc/9hc4/9hc4.fit
pddf	/app/data/pr_computation/hc/9hc4/9hc4.pr.out
profile	/app/data/saxs_computation/hc/9hc4/9hc4.dat

Cryo-EM structure of P. urativorans 70S ribosome with 2 copies of bS20.

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (52)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 polymer

聚合体 Polymer

Entity 34 polymer

聚合体 Polymer

Entity 35 polymer