9lr9

Local reconstruction of bovine adenovirus type 3 capsid

Method: ELECTRON MICROSCOPY D_max: 226.5 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9lr9
沉积日期 deposition_date	2025-01-30
结构标题 title	Local reconstruction of bovine adenovirus type 3 capsid
关键词 keywords	Complex, VIRAL PROTEIN; VIRAL PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	81.86 Å
回转半径 Rg (电子) rg_electron	81.69 Å
零角强度 I(0) i0	29702100000.00
分子量 molecular_weight	1441700.0 kDa
排除体积 excluded_volume	1789300 Å³
包络体积 envelope_volume	2588200 Å³
水化壳体积 shell_volume	252980 Å³
包络直径 envelope_diameter	328.5 Å
壳层 Rg shell_rg	86.90 Å
包络 Rg envelope_rg	81.68 Å
形状 Rg shape_rg	81.67 Å
总 Rg total_rg	81.78 Å
总原子数 total_atoms	101800
残基数 n_residues	12783
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	226.5 Å
Rg (实空间) rg_real	79.19 Å
Rg 误差 (实空间) rg_real_error	0.69 Å
I(0) (实空间) i0_real	2.8600e+10
I(0) 误差 (实空间) i0_real_error	5.3770e+08
Rg (倒空间) rg_reciprocal	81.59 Å
I(0) (倒空间) i0_reciprocal	29680000000.0000
解质量估计 total_estimate	0.6528
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	107.3 Å
偏度 Skewness skewness	0.220
峰度 Kurtosis kurtosis	-0.451
角度范围 angular_range	— – 0.0950 Å⁻¹
当前正则化参数 α current_alpha	1.1500
最高正则化参数 α highest_alpha	2782000000.0000
实空间数据点数 n_real_points	20
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.994; Stabil: 0.974; Sysdev: 0.008; Positv: 1.000; Valcen: 0.950; Smooth: 0.008

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (9)

Entity 1 polymer

描述 description	Hexon protein
分子量 formula_weight	103148.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	12

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,D,F,G,H,I,J,X,Y,g,h,i
原子数 atom_count	101800

MATPSMLPQWSYMHIAGQDASEYLSPGLVQFAQATESYFNIGNKFRNPTVAPTHDVTTERSQRLQLRFVPVDREDTQYSYKTRFQLAVGDNRVLDMASTYFDIRGTLDRGASFKPYSGTAYNSFAPKSAPNNTQFRQANNGHPAQTIAQASYVATIGGANNDLQMGVDERQLPVYANTTYQPEPQLGIEGWTAGSMAVIDQAGGRVLRNPTQTPCYGSYAKPTNEHGGITKANTQVEKKYYRTGDNGNPETVFYTEEADVLTPDTHLVHAVPAADRAKVEGLSQHAAPNRPNFIGFRDCFVGLMYYNSGGNLGVLAGQSSQLNAVVDLQDRNTELSYQMLLANTTDRSRYFSMWNQAMDSYDPEVRVIDNVGVEDEMPNYCFPLSGVQIGNRSHEVQRNQQQWQNVANSDNNYIGKGNLPAMEINLAANLWRSFLYSNVALYLPDNLKFTPHNIQLPPNTNTYEYMNGRIPVSGLIDTYVNIGTRWSPDVMDNVNPFNHHRNSGLRYRSQLLGNGRFCDFHIQVPQKFFAIRNLLLLPGTYTYEWSFRKDVNMILQSTLGNDLRVDGATVNITSVNLYASFFPMSHNTASTLEAMLRNDTNDQSFNDYLSAANMLYPIPPNATQLPIPSRNWAAFRGWSLTRLKQRETPALGSPFDPYFTYSGTIPYLDGTFYLSHTFRKVAIQFDSSVTWPGNDRLLTPNEFEIKISVDGEGYNVAQSNMTKDWFLVQMLANYNIGYQGYHLPPDYKDRTFSFLHNFIPMCRQVPNPATEGYFGLGIVNHRTTPAYWFRFCRAPREGHPYPQLALPPHWDPRHALRDPERKFLCDRTLWRIPFSSNFMSMGSLTDLGQNLLYANAAHALDMTFEMDPINEPTLLYVLFEVFDVARVHQPHRGVIEVVYLRTPFSAGNATT

Entity 2 polymer

描述 description	PVI
分子量 formula_weight	28249.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	9

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B,L,S,T,Z,a,b,c,d

MDEYNYAALAPRQGSRPMLSQWSGIGTHEMHGGRFNLGSLWSGIRNVGSALRTGALGPGTAMRASVARPAEKDGLARKDIEGVSAGIHGAVDLGRQQLEKAIEQRLERRPTAAGVEDLPLPPGTVLEADRLPPSYAEAVAERPPPADVLLPASSKPPVAVVTLPPKKRVSEEPVEEVVIRSSAPPSYDEVMAPQPTLVAEQGAMKAVPVIKPAQPFTPAVHETQRIVTNLPITTAVTRRRGWQGTLNDIVGLGVRTVKRRRCY

Entity 3 polymer

描述 description	Pre-histone-like nucleoprotein
分子量 formula_weight	19002.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C,U,e,f

MAILISPSNNTGWGLGCNKMYGGARIRSDLHPVKVRSHYRAAWGSRTGRVGRRATAALADAVAATGDPVADTIEAVVADARQYRRRRRRGVRRVRRLRRSPRTALQRRVRSVRRQVARARRVGRRAAAIAADAAMAMAAPARRRRNIYWVRDAATGARVPVTTRPTVSNTV

Entity 4 polymer

描述 description	PX
分子量 formula_weight	8514.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MSPRGNLTYRLRIPVALSGRRRRRTGLRGGSAYLLGRRRRRAGGGRLRGGFLPLLAPIIAAAIGAIPGIASVAIQAAHNK

Entity 5 polymer

描述 description	Pre-hexon-linking protein IIIa
分子量 formula_weight	63270.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K

MSDADPRIFQLVSRQETVAMAEAPRSLGPAPSNPSLQALFQSQPSAEQEWHGVLERVMALNKNGDFGSQPQANRFGAILEAVVPPRSDPTHEKVLAIVNALLETQAIRRDEAGQMYTALLQRVARYNSVNVQGNLDRLIQDVKEALAQRERTGPGAGLGSVVALNAFLSTQPAVVERGQENYVAFVSALKLMVTEAPQSEVYQAGPSFFFQTSRHGSQTVNLSQAFDNLRPLWGVRAPVHERTTISSLLTPNTRLLLLLIAPFTDSVGISRDSYLGHLLTLYRETIGNTRVDETTYNEITEVSRALGAEDASNLQATLNYLLTNKQSKLPQEFSLSPEEERVLRYVQQSVSLFLMQDGHTATTALDQAAANIAPSFYASHRDFINRLMDYFQRAAAMAPDYFLQAVMNPHWLPPPGFFTQEFDFPEPNEGFLWDDLDSALLRAHVKEEEDQGAVGGTPAASAPASRAHTPPPPPGAADLFAPNAFRNVQNNGVDELIDGLSRWKTYAQERQEVVERHRRREARRRAREARLESSDDDDSDLGPFLRGTGHLVHNQFMHLKPRGPRQFW

Entity 6 polymer

描述 description	PV
分子量 formula_weight	45388.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M

MASSRLIKEEMLDIVAPEIYKRKRPRRERAAPYAVKQEEKPLVKAERKIKRGSRKRALSGVDVPLPDDGFEDDEPHIEFVSAPRRPYQWKGRRVRRVLRPGVAVSFTPGARSLRPSSKRVYDEVYADDDFLEAAAAREGEFAYGKRGREAAQAQLLPAVAVPEPTYVVLDESNPTPSYKPVTEQKVILSRKRGVGKVEPTIQVLASKKRRMAENEDDRGAGSVAEVQMREVKPVTAALGIQTVDVSVPDHSTPMEVVQSLSRAAQVAQRLTQQQVRPSAKIKVEAMDLSAPVDAKPLDLKPVDVKPTPTFVLPSFRSLSTQTDSLPAAVVVPRKPRVHRATRRTARGLLPYYRLHPSITPTPGYRGSVYTSSGVRLPAVRAPPSPPYPQGDSPPQRCRGRGAAARRALSP

Entity 7 polymer

描述 description	Pre-hexon-linking protein VIII
分子量 formula_weight	23746.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N,R

MSKEIPTPYVWTFQPQMGAAAGASQDYSTRMNWFSAGPDMIHDVNNIRDAQNRILMTQSAITATPRNLIDPRQWAAHLIKQPVVGTTHVEMPRNEVLEQHLTSHGAQIAGGGAAGDYFKSPTSARTLIPLTASCLRPDGVFQLGGGSRSSFNPLQTDFAFHALPSRPRHGGIGSRQFVEEFVPAVYLNPYSGPPDSYPDQFIRHYNVYSNSVSGYS

Entity 8 polymer

描述 description	PIX
分子量 formula_weight	13719.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O,P,Q,V

MAEEGRIYVPYVTARLPKWSGSVQDKTGSNMLGGVVLPPNSQAHRTETVGTEATRDNLHAEGARRPEDQTPYMILVEDSLGGLKRRMDLLEESNQQLLATLNRLRTGLAAYVQANLVGGQVNPFV

Entity 9 polymer

描述 description	Penton protein
分子量 formula_weight	55135.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	j

MLQPELQPIPLSRRRLMRRWWLGPSRRFLFDEIHVPPRYAAASAGRNSIRYSMLPPLYDTTKIYLIDNKSSDIQTLNYQNDHSDYLTTIVQNSDFTPLEASNHSIELDERSRWGGNLKTILYTNLPNITQHMFSNSFRVKMMASKKDGVPQYEWFPLRLPEGNFSETMVIDLMNNAIVELYLALGRQEGVKEEDIGVKIDTRNFSLGYDPQTQLVTPGVYTNEAMHADIVLLPGCAIDFTHSRLNNLLGIRKRFPYQEGFVISYEDLKGGNIPALMDVEEFNKSKTVRALREDPKGRSYHVGEDPEARENETAYRSWYLAYNYGDPEKGVRATTLLTTGDVTCGVEQIYWSLPDMALDPVTFKASLKTSNYPVVGTELLPLVPRSFYNAQAVYSQWIQEKTNQTHVFNRFPENQILVRPPAPTITSISENKPSLTDHGIVPLRNRLGGVQRVTLTDARRRSCPYVYKSLGIVTPQVLSSRTF

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7. 引用文献 (1)

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分辨率 resolution	3.30 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	45262

fit	/app/data/saxs_computation/lr/9lr9/9lr9.fit
pddf	/app/data/pr_computation/lr/9lr9/9lr9.pr.out
profile	/app/data/saxs_computation/lr/9lr9/9lr9.dat

Local reconstruction of bovine adenovirus type 3 capsid

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (9)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)