9mlc

Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome activated elongation complex Composite map T

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase subunit beta

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	I3LGP4_PIG
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–1300； UniProt 1–1300

DNA-directed RNA polymerase II subunit RPB3

OrganismNot specified

UniProt A0A481DF93

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A481DF93_PIG
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–275； UniProt 46–320

RPOL4c domain-containing protein

OrganismNot specified

UniProt A0A8D1GCS3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8D1GCS3_PIG
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–184； UniProt 1–184

DNA-directed RNA polymerase II subunit E

OrganismNot specified

UniProt A0A4X1VTX4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A4X1VTX4_PIG
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–210； UniProt 1–210

DNA-directed RNA polymerases I, II, and III subunit RPABC2

OrganismNot specified

UniProt A0A4X1VEK9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A4X1VEK9_PIG
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–127； UniProt 1–127

DNA-directed RNA polymerase II subunit RPB7

OrganismNot specified

UniProt A0A4X1VKG7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A4X1VKG7_PIG
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–172； UniProt 1–172

DNA-directed RNA polymerases I, II, and III subunit RPABC3

OrganismNot specified

UniProt A0A8D1AQR7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8D1AQR7_PIG
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–150； UniProt 1–150

DNA-directed RNA polymerase II subunit RPB9

OrganismNot specified

UniProt P60899

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB9_PIG
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–125； UniProt 1–125

DNA-directed RNA polymerases I, II, and III subunit RPABC5

OrganismNot specified

UniProt A0A8W4F9W9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8W4F9W9_PIG
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain J； PDBConstruct 1–67； UniProt 1–67

RNA polymerase II subunit J

OrganismNot specified

UniProt A0A8D0TE17

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8D0TE17_PIG
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain K； PDBConstruct 1–117； UniProt 1–117

RNA polymerase II subunit K

OrganismNot specified

UniProt A0A4X1TRS6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A4X1TRS6_PIG
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain L； PDBConstruct 1–58； UniProt 1–58

Transcription elongation factor 1 homolog

Homo sapiens

UniProt P60002

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ELOF1_HUMAN
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain O； PDBConstruct 1–83； UniProt 1–83

RNA polymerase-associated protein CTR9 homolog

Homo sapiens

UniProt Q6PD62

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CTR9_HUMAN
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain Q； PDBConstruct 1–1173； UniProt 1–1173

Transcription elongation factor A protein 1

Homo sapiens

UniProt P23193

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TCEA1_HUMAN
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain S； PDBConstruct 1–301； UniProt 1–301

RNA polymerase-associated protein LEO1

Homo sapiens

UniProt Q8WVC0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	LEO1_HUMAN
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain U； PDBConstruct 1–666； UniProt 1–666

RNA polymerase II-associated factor 1 homolog

Homo sapiens

UniProt Q8N7H5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PAF1_HUMAN
Isoform	—
PDB entities	22
Chains and sequence ranges	Author chain V； PDBConstruct 1–531； UniProt 1–531

WDR61

Homo sapiens

UniProt Q9GZS3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	WDR61_HUMAN
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain W； PDBConstruct 1–305； UniProt 1–305

Parafibromin

Homo sapiens

UniProt Q6P1J9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CDC73_HUMAN
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain X； PDBConstruct 1–531； UniProt 1–531

Transcription elongation factor SPT4

Homo sapiens

UniProt P63272

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT5 × 1 （O00267） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SPT4H_HUMAN
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain Y； PDBConstruct 1–117； UniProt 1–117

Transcription elongation factor SPT5

Homo sapiens

UniProt O00267

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Protein IWS1 homolog × 1 （Q96ST2） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SPT5H_HUMAN
Isoform	—
PDB entities	26
Chains and sequence ranges	Author chain Z； PDBConstruct 1–1087； UniProt 1–1087

Protein IWS1 homolog

Homo sapiens

UniProt Q96ST2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 24 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 DNA-directed RNA polymerase subunit beta × 1 （I3LGP4） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RPOL4c domain-containing protein × 1 （A0A8D1GCS3） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A4X1VEK9） DNA-directed RNA polymerase II subunit RPB7 × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （A0A8D1AQR7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （A0A8W4F9W9） RNA polymerase II subunit J × 1 （A0A8D0TE17） RNA polymerase II subunit K × 1 （A0A4X1TRS6） Transcription elongation factor SPT6 × 1 DNA (194-MER) × 1 Transcription elongation factor 1 homolog × 1 （P60002） ;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ; × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 Transcription elongation factor A protein 1 × 1 （P23193） DNA (194-MER) × 1 RNA polymerase-associated protein LEO1 × 1 （Q8WVC0） RNA polymerase II-associated factor 1 homolog × 1 （Q8N7H5） WDR61 × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 （O00267） ZINC ION × 10 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	IWS1_HUMAN
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain i； PDBConstruct 1–819； UniProt 1–819

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9mlc
Deposition date deposition_date	2024-12-19
Structure title title	Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome activated elongation complex Composite map T
Keywords keywords	polymerase, elongation factor, nucleosome, TRANSCRIPTION; TRANSCRIPTION
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9mlc__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9mlc__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9mlc__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	70.56 Å
Rg (electron density)	69.39 Å
Total Rg	68.85 Å
Atom count	60473
Residues	8413
Excluded volume	1038500 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9mlc__assembly_1__model_1`	27-meric （27）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (29)

Entity 1 Entity 1 polymer

Description description	DNA-directed RNA polymerase II subunit RPB1
Molecular weight formula_weight	218920.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	60473

MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMQLETNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN

Entity 2 Entity 2 polymer

Description description	DNA-directed RNA polymerase subunit beta
Molecular weight formula_weight	147938.6 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MKPLRPENPPKAELKGLLFVQQHCKTRLHSVAVTRPRGAATEESTPKLRMCSTNLSQALAYFRAGANLTAASLWAGFRGSRRIFWERRKRKSLCLALRPGGACWRWLLAVSCVSLRGLGAPGSCANMYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELEGTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Entity 3 Entity 3 polymer

Description description	DNA-directed RNA polymerase II subunit RPB3
Molecular weight formula_weight	31439.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLTSDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPSDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN

Entity 4 Entity 4 polymer

Description description	RPOL4c domain-containing protein
Molecular weight formula_weight	20962.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MWGPAQPYSDSALSPKPRPFRAVFRGSALPFPAVRVEVRGRSMAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY

Entity 5 Entity 5 polymer

Description description	DNA-directed RNA polymerase II subunit E
Molecular weight formula_weight	24644.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ

Entity 6 Entity 6 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC2
Molecular weight formula_weight	14477.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIISD

Entity 7 Entity 7 polymer

Description description	DNA-directed RNA polymerase II subunit RPB7
Molecular weight formula_weight	19314.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

Entity 8 Entity 8 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC3
Molecular weight formula_weight	17162.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF

Entity 9 Entity 9 polymer

Description description	DNA-directed RNA polymerase II subunit RPB9
Molecular weight formula_weight	14541.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

Entity 10 Entity 10 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC5
Molecular weight formula_weight	7655.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNYAPLEK

Entity 11 Entity 11 polymer

Description description	RNA polymerase II subunit J
Molecular weight formula_weight	13310.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEGIE

Entity 12 Entity 12 polymer

Description description	RNA polymerase II subunit K
Molecular weight formula_weight	7018.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MDTQKDVQPPKQQPMIYICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR

Entity 13 Entity 13 polymer

Description description	Transcription elongation factor SPT6
Molecular weight formula_weight	199330.7 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M

MSDFVESEAEESEEEYNDEGEVVPRVTKKFVEEEDDDEEEEEENLDDQDEQGNLKGFINDDDDEDEGEEDEGSDSGDSEDDVGHKKRKRTSFDDRLEDDDFDLIEENLGVKVKRGQKYRRVKKMSDDEDDDEEEYGKEEHEKEAIAEEIFQDGEGEEGQEAMEAPMAPPEEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEEYEYEDDEAEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKGAEDDELEEEADWIYRNAFATPTISLQESCDYLDRGQPASSFSRKGPSTIQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRVREEGDEEGEGDEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKICLAEDEGLLTTDISIDLKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLYNWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLLNKKPHVVTVAGENRDAQMLIEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYIEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSDGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSSRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR

Source Source

Source organism organism	Homo sapiens
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 14 Entity 14 polymer

Description description	DNA (194-MER)
Molecular weight formula_weight	63984.7 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	N

TTGTGTTTGGTGTGTCTGGGTGGTGGCAATTTTCGATGTATATATCTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTGATATCGCGCGTGATCTTACGGCATTATACGTA

Entity 15 Entity 15 polymer

Description description	Transcription elongation factor 1 homolog
Molecular weight formula_weight	9475.9 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

MGRRKSKRKPPPKKKMTGTLETQFTCPFCNHEKSCDVKMDRARNTGVISCTVCLEEFQTPITYLSEPVDVYSDWIDACEAANQ

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 16 Entity 16 polymer

Description description	;RNA (5'-R(PUPUPUPGPGPUPGPUPGPUPCPUPGPGPGPUPGPGPUPG)-3') ;
Molecular weight formula_weight	12544.3 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	P

GCAUCACUGUCUUGUGUUUGGUGUGUCUGGGUGGUGGCA

Entity 17 Entity 17 polymer

Description description	RNA polymerase-associated protein CTR9 homolog
Molecular weight formula_weight	133715.4 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MSRGSIEIPLRDTDEVIELDFDQLPEGDEVISILKQEHTQLHIWIALALEYYKQGKTEEFVKLLEAARIDGNLDYRDHEKDQMTCLDTLAAYYVQQARKEKNKDNKKDLITQATLLYTMADKIIMYDQNHLLGRACFCLLEGDKMDQADAQFHFVLNQSPNNIPALLGKACISFNKKDYRGALAYYKKALRTNPGCPAEVRLGMGHCFVKLNKLEKARLAFSRALELNSKCVGALVGLAVLELNNKEADSIKNGVQLLSRAYTIDPSNPMVLNHLANHFFFKKDYSKVQHLALHAFHNTEVEAMQAESCYQLARSFHVQEDYDQAFQYYYQATQFASSSFVLPFFGLGQMYIYRGDKENASQCFEKVLKAYPNNYETMKILGSLYAASEDQEKRDIAKGHLKKVTEQYPDDVEAWIELAQILEQTDIQGALSAYGTATRILQEKVQADVPPEILNNVGALHFRLGNLGEAKKYFLASLDRAKAEAEHDEHYYNAISVTTSYNLARLYEAMCEFHEAEKLYKNILREHPNYVDCYLRLGAMARDKGNFYEASDWFKEALQINQDHPDAWSLIGNLHLAKQEWGPGQKKFERILKQPSTQSDTYSMLALGNVWLQTLHQPTRDREKEKRHQDRALAIYKQVLRNDAKNLYAANGIGAVLAHKGYFREARDVFAQVREATADISDVWLNLAHIYVEQKQYISAVQMYENCLRKFYKHQNTEVVLYLARALFKCGKLQECKQTLLKARHVAPSDTVLMFNVALVLQRLATSVLKDEKSNLKEVLNAVKELELAHRYFSYLSKVGDKMRFDLALAATEARQCSDLLSQAQYHVARARKQDEEERELRAKQEQEKELLRQKLLKEQEEKRLREKEEQKKLLEQRAQYVEKTKNILMFTGETEATKEKKRGGGGGRRSKKGGEFDEFVNDDTDDDLPISKKKKRRKGSGSEQEGEDEEGGERKKKKRRRHPKGEEGSDDDETENGPKPKKRRPPKAEKKKAPKPERLPPSMKGKIKSKAIISSSDDSSDEDKLKIADEGHPRNSNSNSDSDEDEQRKKCASSESDSDENQNKSGSEAGSPRRPRRQRSDQDSDSDQPSRKRRPSGSEQSDNESVQSGRSHSGVSENDSRPASPSAESDHESERGSDNEGSGQGSGNESEPEGSNNEASDRGSEHGSDDSD

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 18 Entity 18 polymer

Description description	RNA polymerase-associated protein RTF1 homolog
Molecular weight formula_weight	80460.8 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MRGRLCVGRAAAAAAAVAVPLAGGQEGSPGGGRRGSRGTTMVKKRKGRVVIDSDTEDSGSDENLDQELLSLAKRKRSDSEEKEPPVSQPAASSDSETSDSDDEWTFGSNKNKKKGKARKIEKKGTMKKQANKTASSGSSDKDSSAESSAPEEGEVSDSDSNSSSSSSDSDSSSEDEEFHDGYGEDLMGDEEDRARLEQMTEKEREQELFNRIEKREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKRDEKLDKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEEDDKSSEKSDRSSRTSSSDEEEEKEEIPPKSQPVSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGNDQRVFRLEFVSNQEFTESEFMKWKEAMFSAGMQLPTLDEINKKELSIKEALNYKFNDQDIEEIVKEKERFRKAPPNYAMKKTQLLKEKAMAEDLGDQDKAKQIQDQLNELEERAEALDRQRTKNISAISYINQRNREWNIVESEKALVAESHNMKNQQMDPFTRRQCKPTIVSNSRDPAVQAAILAQLNAKYGSGVLPDAPKEMSKGQGKDKDLNSKSASDLSEDLFKVHDFDVKIDLQVPSSESKALAITSKAPPAKDGAPRRSLNLEDYKKRRGLI

Source Source

Source organism organism	Homo sapiens
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 19 Entity 19 polymer

Description description	Transcription elongation factor A protein 1
Molecular weight formula_weight	34022.7 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

MEDEVVRFAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQSTRIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPEAREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 20 Entity 20 polymer

Description description	DNA (194-MER)
Molecular weight formula_weight	66042.1 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	T

TACGTATAATGCCGTAAGATCACGCGCGATATCAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCGAAAATTGCCACCACCCAGACACACCAAACACAAGACAGTGAT

Entity 21 Entity 21 polymer

Description description	RNA polymerase-associated protein LEO1
Molecular weight formula_weight	75514.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MADMEDLFGSDADSEAERKDSDSGSDSDSDQENAASGSNASGSESDQDERGDSGQPSNKELFGDDSEDEGASHHSGSDNHSERSDNRSEASERSDHEDNDPSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHSDDEKWGREDKSDQSDDEKIQNSDDEERAQGSDEDKLQNSDDDEKMQNTDDEERPQLSDDERQQLSEEEKANSDDERPVASDNDDEKQNSDDEEQPQLSDEEKMQNSDDERPQASDEEHRHSDDEEEQDHKSESARGSDSEDEVLRMKRKNAIASDSEADSDTEVPKDNSGTMDLFGGADDISSGSDGEDKPPTPGQPVDENGLPQDQQEEEPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESISLAAIKNRYKGGIREERARIYSSDSDEGSEEDKAQRLLKAKKLTSDEEGEPSGKRKAEDDDKANKKHKKYVISDEEEEDDD

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 22 Entity 22 polymer

Description description	RNA polymerase II-associated factor 1 homolog
Molecular weight formula_weight	60052.7 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V

MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMETEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESEREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAQGGSDNDSDSGSNGGGQRSRSHSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 23 Entity 23 polymer

Description description	WDR61
Molecular weight formula_weight	33617.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	W

MTNQYGILFKQEQAHDDAIWSVAWGTNKKENSETVVTGSLDDLVKVWKWRDERLDLQWSLEGHQLGVVSVDISHTLPIAASSSLDAHIRLWDLENGKQIKSIDAGPVDAWTLAFSPDSQYLATGTHVGKVNIFGVESGKKEYSLDTRGKFILSIAYSPDGKYLASGAIDGIINIFDIATGKLLHTLEGHAMPIRSLTFSPDSQLLVTASDDGYIKIYDVQHANLAGTLSGHASWVLNVAFCPDDTHFVSSSSDKSVKVWDVGTRTCVHTFFDHQDQVWGVKYNGNGSKIVSVGDDQEIHIYDCPI

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 24 Entity 24 polymer

Description description	Parafibromin
Molecular weight formula_weight	60673.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X

MADVLSVLRQYNIQKKEIVVKGDEVIFGEFSWPKNVKTNYVVWGTGKEGQPREYYTLDSILFLLNNVHLSHPVYVRRAATENIPVVRRPDRKDLLGYLNGEASTSASIDRSAPLEIGLQRSTQVKRAADEVLAEAKKPRIEDEECVRLDKERLAARLEGHKEGIVQTEQIRSLSEAMSVEKIAAIKAKIMAKKRSTIKTDLDDDITALKQRSFVDAEVDVTRDIVSRERVWRTRTTILQSTGKNFSKNIFAILQSVKAREEGRAPEQRPAPNAAPVDPTLRTKQPIPAAYNRYDQERFKGKEETEGFKIDTMGTYHGMTLKSVTEGASARKTQTPAAQPVPRPVSQARPPPNQKKGSRTPIIIIPAATTSLITMLNAKDLLQDLKFVPSDEKKKQGCQRENETLIQRRKDQMQPGGTAISVTVPYRVVDQPLKLMPQDWDRVVAVFVQGPAWQFKGWPWLLPDGSPVDIFAKIKAFHLKYDEVRLDPNVQKWDVTVLELSYHKRHLDRPVFLRFWETLDRYMVKHKSHLRF

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 25 Entity 25 polymer

Description description	Transcription elongation factor SPT4
Molecular weight formula_weight	13210.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y

MALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 26 Entity 26 polymer

Description description	Transcription elongation factor SPT5
Molecular weight formula_weight	121145.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Z

MSDSEDSNFSEEEDSERSSDGEEAEVDEERRSAAGSEKEEEPEDEEEEEEEEEYDEEEEEEDDDRPPKKPRHGGFILDEADVDDEYEDEDQWEDGAEDILEKEEIEASNIDNVVLDEDRSGARRLQNLWRDQREEELGEYYMKKYAKSSVGETVYGGSDELSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPLQIKSVVAPEHVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVRLKRGIYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARMSLKDWFAKRKKFKRPPQRLFDAEKIRSLGGDVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTESTGKEREHNFQPGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMGDHVKVIAGRFEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELVQLDPQTVGVIVRLERETFQVLNMYGKVVTVRHQAVTRKKDNRFAVALDSEQNNIHVKDIVKVIDGPHSGREGEIRHLFRSFAFLHCKKLVENGGMFVCKTRHLVLAGGSKPRDVTNFTVGGFAPMSPRISSPMHPSAGGQRGGFGSPGGGSGGMSRGRGRRDNELIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVGSRRPGGMTSTYGRTPMYGSQTPMYGSGSRTPMYGSQTPLQDGSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPTPSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQGSYQPSPSPQSYHQVAPSPAGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMDLDEQLKILNLRFLGKLLEA

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 27 Entity 27 polymer

Description description	Protein IWS1 homolog
Molecular weight formula_weight	92094.8 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	i

MDSEYYSGDQSDDGGATPVQDERDSGSDGEDDVNEQHSGSDTGSVERHSENETSDREDGLPKGHHVTDSENDEPLNLNASDSESEELHRQKDSDSESEERAEPPASDSENEDVNQHGSDSESEETRKLPGSDSENEELLNGHASDSENEDVGKHPASDSEIEELQKSPASDSETEDALKPQISDSESEEPPRHQASDSENEEPPKPRMSDSESEELPKPQVSDSESEEPPRHQASDSENEELPKPRISDSESEDPPRHQASDSENEELPKPRISDSESEDPPRNQASDSENEELPKPRVSDSESEGPQKGPASDSETEDASRHKQKPESDDDSDRENKGEDTEMQNDSFHSDSHMDRKKFHSSDSEEEEHKKQKMDSDEDEKEGEEEKVAKRKAAVLSDSEDEEKASAKKSRVVSDADDSDSDAVSDKSGKREKTIASDSEEEAGKELSDKKNEEKDLFGSDSESGNEEENLIADIFGESGDEEEEEFTGFNQEDLEEEKGETQVKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMSGKRRRNRDGGTFISDADDVVSAMIVKMNEAAEEDRQLNNQKKPALKKLTLLPAVVMHLKKQDLKETFIDSGVMSAIKEWLSPLPDRSLPALKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLINEWSRPIFGLTSNYKGMTREEREQRDLEQMPQRRRMNSTGGQTPRRDLEKVLTGEEKALRPGDPGFCARARVPMPSNKDYVVRPKWNVEMESSRFQATSKKGISRLDKQMRKFTDIRKKSRSAHAVKISIEGNKMPL

Source Source

Source organism organism	Homo sapiens
Common name common_name	Human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 28 Entity 28 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	man
Copy count entity_count	10

Source Source

Source organism organism	Xenopus laevis
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli

Entity 29 Entity 29 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	man
Copy count entity_count	1

Source Source

Source organism organism	Xenopus laevis
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	2.40 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	762523

Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome activated elongation complex Composite map T

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase subunit beta

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

RPOL4c domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit E

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB9

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

RNA polymerase II subunit J

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer