9nbm

Open conformation of ArsA from L. ferriphilum in complex with MgADP determined in absence of arsenite

Method: ELECTRON MICROSCOPY D_max: 77.4 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9nbm
沉积日期 deposition_date	2025-02-14
结构标题 title	Open conformation of ArsA from L. ferriphilum in complex with MgADP determined in absence of arsenite
关键词 keywords	ATPase, open conformation, arsenite, arsenic, ADP, Intradimeric Walker A, HYDROLASE; HYDROLASE
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	24.95 Å
回转半径 Rg (电子) rg_electron	23.89 Å
零角强度 I(0) i0	60743300.00
分子量 molecular_weight	60054.0 kDa
排除体积 excluded_volume	75150 Å³
包络体积 envelope_volume	92704 Å³
水化壳体积 shell_volume	31544 Å³
包络直径 envelope_diameter	79.5 Å
壳层 Rg shell_rg	31.82 Å
包络 Rg envelope_rg	23.76 Å
形状 Rg shape_rg	23.91 Å
总 Rg total_rg	24.70 Å
总原子数 total_atoms	4220
残基数 n_residues	552
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	77.4 Å
Rg (实空间) rg_real	24.77 Å
Rg 误差 (实空间) rg_real_error	0.41 Å
I(0) (实空间) i0_real	6.0740e+07
I(0) 误差 (实空间) i0_real_error	7.3480e+05
Rg (倒空间) rg_reciprocal	24.81 Å
I(0) (倒空间) i0_reciprocal	60750000.0000
解质量估计 total_estimate	0.9024
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	31.9 Å
偏度 Skewness skewness	0.151
峰度 Kurtosis kurtosis	-0.477
角度范围 angular_range	— – 0.3200 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	17620000.0000
实空间数据点数 n_real_points	64
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.913; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.994

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (3)

Entity 1 polymer

描述 description	Arsenite transporter ATPase-like protein,arsA
分子量 formula_weight	63972.0 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	4220

MGMKFLQLPPRFMFFTGKGGVGKTSIACATSIQLANAGKRVLLVSTDPASNVGQVFGVDIGNRVTPIPAVPHLSALEIDPEAAASAYRERLVGPVRGVLPDDVVKGIEESLSGACTTEIAAFDEFTALLTNAVLTADYQHIIFDTAPTGHTIRLLQLPGAWSGFLEAGKGDASCLGPLAGLEKQRTQYKAAVEALADPLQTRLVLVARAQQATLREVARTHEELATIGIKQQHLVINGILPSAEAANDPLAAAIHEREQTALKNIPATLTSLPRDLVQLKPFNLVGLDALRQLLTDLPLQAHVAADAPIELDEPGMGDLVDGIEADGHGLVMLMGKGGVGKTTLAAAIAVELAHRGLPVHLTTSDPAAHLTDTLEASLDNLTVSRIDPHAETERYRQHVLETKGAQLDAEGRALLEEDLHSPCTEEIAVFQAFSRIIREAGKKFVVMDTAPTGHTLLLLDATGAYHREVRRQMGNKGTHFTTPMMQLRDPNQTKVLVVTLAETTPVLEAAKLQADLRRAGIEPWAWIINTSVAAASAKSPLLRQRAANELREINAVANHHADRYAVVPLLKEEPIGAERLRALIHPQTHHHHHH

来源 Source

来源生物 organism	Leptospirillum ferriphilum
NCBI 分类编号 ncbi_taxonomy_id	1048260
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

描述 description	ADENOSINE-5'-DIPHOSPHATE
分子量 formula_weight	427.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

Entity 3 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.80 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	34966

fit	/app/data/saxs_computation/nb/9nbm/9nbm.fit
pddf	/app/data/pr_computation/nb/9nbm/9nbm.pr.out
profile	/app/data/saxs_computation/nb/9nbm/9nbm.dat