9nw3

Ciliary tip central pair

Method: ELECTRON MICROSCOPY D_max: 347.5 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9nw3
沉积日期 deposition_date	2025-03-21
结构标题 title	Ciliary tip central pair
关键词 keywords	cilia, microtubules, central pair, PROTEIN BINDING; PROTEIN BINDING
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	143.10 Å
零角强度 I(0) i0	291851000000.00
分子量 molecular_weight	4540400.0 kDa
排除体积 excluded_volume	5638800 Å³
包络体积 envelope_volume	12519000 Å³
水化壳体积 shell_volume	717130 Å³
包络直径 envelope_diameter	451.7 Å
壳层 Rg shell_rg	155.80 Å
包络 Rg envelope_rg	129.70 Å
形状 Rg shape_rg	143.20 Å
总 Rg total_rg	143.10 Å
总原子数 total_atoms	318666
残基数 n_residues	40476
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	347.5 Å
Rg (实空间) rg_real	139.60 Å
Rg 误差 (实空间) rg_real_error	0.60 Å
I(0) (实空间) i0_real	2.7970e+11
I(0) 误差 (实空间) i0_real_error	5.2370e+09
Rg (倒空间) rg_reciprocal	158.10 Å
I(0) (倒空间) i0_reciprocal	310500000000.0000
解质量估计 total_estimate	0.8969
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	221.1 Å
偏度 Skewness skewness	-0.256
峰度 Kurtosis kurtosis	-0.621
角度范围 angular_range	— – 0.0550 Å⁻¹
当前正则化参数 α current_alpha	0.8210
最高正则化参数 α highest_alpha	17360000000.0000
实空间数据点数 n_real_points	12
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.001; Oscil: 0.931; Stabil: 0.972; Sysdev: 1.000; Positv: 1.000; Valcen: 0.959; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (13)

Entity 1 polymer

描述 description	Tubulin alpha chain
分子量 formula_weight	49639.0 kDa
EC 编号 ec_number	3.6.5.-
来源方法 src_method	nat
拷贝数 entity_count	39

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AA,AC,AE,BA,BC,BE,CA,CC,CE,DA,DC,DE,EA,EC,EE,FA,FC,FE,GA,GC,GE,HA,HC,HE,IA,IC,IE,JA,JC,JE,KA,KC,KE,LA,LC,LE,MA,MC,ME
原子数 atom_count	318666

MREVISIHVGQGGIQVGNACWELFCLEHGIQPDGQMPSDKTIGGGDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNSVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTAVVEPYNSILSTHSLLEHTDVAVMLDNEAIYDICRRNLDIERPTYTNLNRLIAQVISSLTASLRFDGALNVDITEFQTNLVPYPRIHFMLSSYAPIISAEKAYHEQLSVAEITNSAFEPANMMAKCDPRHGKYMACSMMYRGDVVPKDVNASIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVMRAVCMISNSTAIAEVFSRLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGIETAEGEGEEEGY

Entity 2 polymer

描述 description	Tubulin beta chain
分子量 formula_weight	49617.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	39

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	AB,AD,AF,BB,BD,BF,CB,CD,CF,DB,DD,DF,EB,ED,EF,FB,FD,FF,GB,GD,GF,HB,HD,HF,IB,ID,IF,JB,JD,JF,KB,KD,KF,LB,LD,LF,MB,MD,MF

MREIVHIQGGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGRYVPRAILMDLEPGTMDSVRAGPFGQLFRPDNFVFGQTGAGNNWAKGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGSGMGTLLISKVREEYPDRIMETFSVVPSPKVSDTVVEPYNATLSVHQLVENADECMVIDNEALYDICFRTLKLTTPTYGDLNHLVSAAMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMIGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMCAADPRHGRYLTASALFRGRMSTKEVDEQMLNVQNKNSSYFVEWIPNNIKSSICDIPPKGLKMAVTFVGNSTAIQEMFKRVAEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEEGEN

Entity 3 polymer

描述 description	Sperm flagellar protein
分子量 formula_weight	24702.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1A,1B,1C

MDAPPLNEEELNQIYNWIDGIPLSRPKKNITRDFADGCMLAEVISHYCPKLVEVHNYSQAHSVTQKLYNWNTLNQRVLKKMGFQISKNDIDCIINAVPDAVERVLKVCQIKLDKYLDDQKKKKDQQQYIENAQMMQNGNKLQLGNANNPYQYAEFLNPKEVSQMLFQRDTTINELRATIEILELKIKKMDQLVKLKDSKIQSLINKLSQAGLS

Entity 4 polymer

描述 description	TLP1
分子量 formula_weight	88103.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2A,2B,2C

MSEENNENQENQYNDDDQYNHNEENEDNEQQEQVDYANHDNDENQEHDPANEEEQGEQNENVNNDNNNNYDEEEDGKRNELDDYLSGGGSNRNDKQDQQSSSKRGSNFGVINKPDDNENYLRNKDILDNFFKQYQGEVQNYFNEFQQKQIQDSQENGNNNFYSKQNNQQSQKEVSIVASDVPLVLRISFYYDNITFHPTKNDQEELFNKPNSYKSIMDQLQKFFAVRRNIRNFDPNETKVTYSDFVEFSYFFIENNDDLFLTQQGKEELWIQQQQESQLEKELRENIDKYENMLKKTTNPQEKLIIQNTIENLKQQLTLLLNKDQGKKGKNLTKEQIRMKNLKEAFNFYCKQQNVTGVAFTFDRIVHEQNVINLACFMLFLKQFNILNKNKYVTKREIQIMFKKYALNYKELDLDHFKIMIEKVAIAFYKEEENLSNIDRVEKLYQYIEIDNIHKFRSKFGLLNQPFNIQVKDGFRLLPFDQGRDIVLKKVDPVVRQKVKQFQEFKKSQQALQIMQLDQKNINQSKLAPRSMENNRRSTPNLQQSSVYSQPGGIGSYANKKIKNQIGKLDAGGKVSWQQLEQLSFSDMKNLNNGQFRPTDLFNENDDEEDKIYLAEYNLQEDRQRKIQEQMVNKKGQENYGQIKEPVRPSLRYLQKHDPNYGKNGYSPSVGNLHRVHASAPEYYSPQNHMNQKQLAINQSYIQRAQQVDQDQRMKEQAMMQKIMNNHDLKVQRGINAMNKRK

Entity 5 polymer

描述 description	C2 domain protein
分子量 formula_weight	57465.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3A,3B,3C

MPQKVSITLKNIEVKRKEQKFKSMHPWAVVMFDGQKKTTQPVQNGDLQPEFNQTWVFQRNSDVNITVSIYKKEYIYDALKNEKEIDLLIGKGKLNILSVLKKAIQQPQEIMVDLVYDFDNKLAAQAYLTIHIQQGADLEEYRNKEQIKQIKSKYESCLTVTNLQLSLDSFKPEAYYYLKIRNSNGSHKTTPQNGGASMMFQGDFDIYFRGQKIVYIDVQEVLKDEILDFITADDLVGIGTLDFEKVDINQSQQRAVIRHKGKQIGSVQCQASYEIFKQEQPEEHASMIRQSNDELLEQIKNLEKKDKFWLSPERYQLDLKLKKKQILDRRQMLMKNRSSAPQLGNESYLFMKPDERNIVQKPDIVISTTTVKLPKLKTNNNVEVPTQYSKQYFRDIHGVGFSTVESSSSLQRSVPKFSIPEQKRFSDPKDIFPGPGRYGTSGVANDLYLPKKFQKITAISEVRTTKQERKERVQIDTGLKKPIDRQQQQTTQNPK

Entity 6 polymer

描述 description	CFAP213
分子量 formula_weight	37998.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	4

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	4A,4B,4C,4D

MYQNQYQQPQQQYGYNQGQQGQQYSQAYQQQPYQQQQGSPYQQQQNYPQGYGAQQQAYQQQQQGYAQQQGYPAQNQQYYQEDYDSLQQYQDNFNSVQPRTRLEREAMKDKESIEKTRINQRVGYETRNTDVKQLLHNPDPKSTLFIPENQRFDKDFSVFDKQQRDQRFATKEVALEKHRIEALERESKRWEQMENQVNKEQVKRQFQAEVLKAGKRNTNGMPFNPITLEYEKSSAGDSLKKRDEMAKVRGYVRAENLDTRSNCGYNILTGEQRIGVEYLVPNHLRDDYKTKVDLKNEFYSIKYKGEQQNQQNQNKYY

Entity 7 polymer

描述 description	TLP2
分子量 formula_weight	205367.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	5A,5B,5C

MQIGEDIYNFLQVFNLINPTEGKKISDDKYSIGKNTTNQLENGQIFAKIIRQIAKIQAQSQKRPEQPFPDLDSLKEMNSPAARLYNWNVLQECFRKLQINLETETKSLIIAGDQDQLSDFIKDIIANVAIYMPELFGKNKGKSKVKQNEYKDEIEVTKVDINKDLTKCKSCLEFFVVLLSRHLSLPPQQTASLFTHNNKYLAHLFAKGVKGLFDPIIFFYQEVYQSIPLLLQLFLEDPTKKSMHFSMNCLKPGLVSKSYEVATWAARLFSKLALEFSESNLLTVSWDWFVGENGGLNTTLLGLKRHPDMKDLVVQILLQFARYNFVELFTIHMKKAQPDPKDLVGTYLVLLKPLTATPSACDEILNAGILDEWIDFALDGSTDEYKNTIDMRTVSLTLLVEVWMLFPFKIEDSEYKSNAVLQQLGKACKDKSQSLIVCSLTLLFQLLIYFGTQKSQFAPNVYRTLTLSIIENHQNVVVREFIMNNFITVYETLESIPLNILIEPLVRQIQVTDNVSYFFNVFDFNFFAYISKNEKLQLKNAIQLLDLFFKIYLNNTIFANVSSVPILNIVNRFIDSETLQEFVIKFVKVALAMFYASEKKKRPKEKVMPLYNNKSAIGQPNLSPGEIEQELIQAQKRALIVEMIKSIVTINCYELNEKIKPLVAHTNIQIKQFTKQNNKGMQSILTLFGNPDTILEKYEKEYIEHQQQLKQEEKERREQEQNDSLLQDFEGSPKNQGDVLKSIEDNLKKYGIGLKERQTKLTKAEATRLALLRNPKADPKILKKLQEIKVNYENREEKKKLEVVKQEQETQKEKEVLRKQLMRRSLEQGVSNFNQRDAEGVLLFQFGSREKQIKRENKTGLPMIQYIDLDKEEQRDKDTINILLRRYNKILKNVFVKYSNTGFSAQFSNKNVNSFDAIKEHNSLISIPEMYRFVKDYELSDKLSKEEHQTLVRIVSQEQAKKKNKQEVSRDQPEQNKQIDQKKKLPPATQSQQNMFLKGSNENKWNFNQNEVKDVKSFDFQGFLDYIVQFAGYIYSKPPEDLRHLPLSASLQKVFDHMREITSKKGQSTALFDDYDNLFFGETEVIKQFNKKLEENPDYPLPQGYKKVKDREIKFEYALDPKGILPMKESFKYAYLVLNDLFQEQLQFSIIEPKSATKEVVKAQPLIGITSDVESHYFDMYKSPQVLEQKRVKKPQMVQSQSVDILGPWGKPDSTKTLPSTLNVKSKYGNQRVLDIDNYRKLDLGLKLAVSTIGYRDRYTAEQCAYCLDDIIKSIETGDSNQAFRNKKVENKVIKEKKKEEEAQEEAKKKYEEKRLKDHDFVMSRAKEIIEQNSDKDKQKKEKELQLKKQKEEEQKKKADDMSSFWKEKKEVFDQQKQKLIEEQKKIQEEQLRRMQEEKEKKEKEFQEFNKKQIEKQQEEFKKQKEKEQQEAEKQRNLEEYQKKLKEKLHMHIVKTDQERIEIEKVSNVKIKELFQKDDVQSVYKTNEFKLSQLFFYLKKYTYREISRQNIADNEISYKTFNWFCYRFNIYPEIISNPKDILLIYRSVTRNKQVIDHKPIGLSEEEFKEAMLRISIKGKKIFNKFSEQLQKGINLNESEMAKIADDENKESNQEEFQENKSVKSSKTEALERMKNVIDYYGNIDEANSHTLEALIYYLGLPNDKLGINEALKNVMEQGAVPDGKLKEAMKQKIVKDPNEILRYNPPQNKVKFGRSSQVGHNQSQMANNSQVGSQNGLAENQENQDQEENEQEQN

Entity 8 polymer

描述 description	Cation channel family protein
分子量 formula_weight	39902.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	6A,6B,6C

MSQKTIQSQEELEENNKNQEEEENVPQQSNKQGSSENLRQKNRSQENLKQRKQSPNYYQSSMVQSGVGTSPTRKPNDKTFITLQKKEEESQVWKPYDFLFPAGRLAKLVKVFDVYFKNFPFSDLLNKLKLTEGYPIVNLIGAAQSNRGKFYAGLARACFNSEAIIVDSAIETGLEPYVIRRDLKLVGVAPENCVKYPKINSTYKDPYELSNGHTHLFLVNDKDKTLQWSQETLFKIQLILKLAEGKVSKKGPRCKIVNVLLADTPNYLDEVRLAVKYDLPIIVCKGSHICDEIIKNSKAQEEEKQWNLVDDEMQELLQKGHFYMLDSLDSEDIAQYIHFFLTFTPY

Entity 9 polymer

描述 description	Dpy-30 motif protein
分子量 formula_weight	11293.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	7A,7B,7C,7D,7E,7F

MAEKKKQQQSKGSSQGLQNIADEDDEQNQNQDQDDGLLTGASNQQGGGGSKFQQLPLRDYYESSVTQVLLEGLRELGAKRPENPIQFLGKFLLDRDPEKKK

Entity 10 polymer

描述 description	Unknown
分子量 formula_weight	3252.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	27

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	8A,8B,8C,8D,8E,8F,8G,8H,8I,8J,8K,8L,8M,8N,8O,8P,8Q,8R,8S,8T,8U,8V,8W,8X,8Y,8Z,9A

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 11 non-polymer

描述 description	GUANOSINE-5'-TRIPHOSPHATE
分子量 formula_weight	523.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	39

Entity 12 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	39

Entity 13 non-polymer

描述 description	GUANOSINE-5'-DIPHOSPHATE
分子量 formula_weight	443.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	39

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.70 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	66174

fit	/app/data/saxs_computation/nw/9nw3/9nw3.fit
pddf	/app/data/pr_computation/nw/9nw3/9nw3.pr.out
profile	/app/data/saxs_computation/nw/9nw3/9nw3.dat

Ciliary tip central pair

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (13)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 non-polymer

Entity 12 non-polymer

Entity 13 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)