9nwi

Pseudomonas phage Pa223 tail (C6 symmetry)

Method: ELECTRON MICROSCOPY D_max: 213.5 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9nwi
沉积日期 deposition_date	2025-03-23
结构标题 title	Pseudomonas phage Pa223 tail (C6 symmetry)
关键词 keywords	Constituent protein, Structural protein, Phage tail component, VIRUS; VIRUS
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	74.89 Å
回转半径 Rg (电子) rg_electron	74.85 Å
零角强度 I(0) i0	11314300000.00
分子量 molecular_weight	919910.0 kDa
排除体积 excluded_volume	1155400 Å³
包络体积 envelope_volume	1835600 Å³
水化壳体积 shell_volume	206380 Å³
包络直径 envelope_diameter	233.7 Å
壳层 Rg shell_rg	76.27 Å
包络 Rg envelope_rg	71.37 Å
形状 Rg shape_rg	74.88 Å
总 Rg total_rg	74.74 Å
总原子数 total_atoms	65094
残基数 n_residues	8040
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	213.5 Å
Rg (实空间) rg_real	74.71 Å
Rg 误差 (实空间) rg_real_error	0.89 Å
I(0) (实空间) i0_real	1.1300e+10
I(0) 误差 (实空间) i0_real_error	2.3320e+08
Rg (倒空间) rg_reciprocal	74.82 Å
I(0) (倒空间) i0_reciprocal	11310000000.0000
解质量估计 total_estimate	0.8380
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	91.5 Å
偏度 Skewness skewness	0.342
峰度 Kurtosis kurtosis	-0.362
角度范围 angular_range	— – 0.1050 Å⁻¹
当前正则化参数 α current_alpha	0.0175
最高正则化参数 α highest_alpha	437200000.0000
实空间数据点数 n_real_points	22
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.956; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.991; Smooth: 0.032

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Head-to-Tail adapter
分子量 formula_weight	24105.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	24

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P,Q,R,S,T,U,V,W,X
原子数 atom_count	65094

MATINNVTDLAIAAIQWSDRQDLTQELLMLFIGNTTDRLNRLLRVRENEHFETLMAFGGGIEIPEHFVALRSITGDSLIGGRTLQYITQDIFTHYVNYNYQPQGVTYYTRLGNFWRVFPVVPDGAPFIVNYWTVLPELSLANPTTWALTKYPQIYLYGVLEQIYLYTMDEARSQFWGQKLERAVMELQNEENAADFASTRLAIKDIER

Entity 2 polymer

描述 description	Capsid and scaffold protein
分子量 formula_weight	57719.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Y,Z,a,b,c,d

MALERQEVKNPTGIVTDIAPADLPLEKWSFGNNVRFKNGKAQKALGHTPIFDTAQAPILDMFPFIRNNIPYWLLCGEQRMYLADGTTVVDVSPGGHSASVTSRWSSGSFNGVIFANNPSNYPYVLMPQNSGFIPMPNWPANTFAKRMKSFKNFMIALNVTQNSVEMPQMVWWSTSADAGGIPVSWDPTDPTKDAGQNTLADTNGAIVDGVKLRDSFIIYKEDSVYSMRYIGGLFIFQFQQLFNDVGILGPNCAIEFDGNHFVVGHGDVYVHNGVQKQSVIDAQVRKFFFSDINPDNYQRTFVIADHVNTEMWVCYSSTRSEPGKHCDRAIIWNWKENTWSIRDLPNVLSGAYGIIDPKVSNLWDDDPNPWDTYTSVWGEGSYNPAKSSMIFSSFQDKKLFLFGNNSTFSGQNFVSTLERSDIYLGDDRMMKTVSAIIPHITGNGTCNIWVGNAQVQGSGIRWKGPYPYRIGQDYKIDTKHVGRYIALKFDFSSEGDWYFNGYTIEMAPKAGMR

▶

7. 引用文献 (1)

▶

分辨率 resolution	2.80 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	46000

fit	/app/data/saxs_computation/nw/9nwi/9nwi.fit
pddf	/app/data/pr_computation/nw/9nwi/9nwi.pr.out
profile	/app/data/saxs_computation/nw/9nwi/9nwi.dat

Pseudomonas phage Pa223 tail (C6 symmetry)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)