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9q1j

Cryo-EM structure of SARS-CoV-2 nsp10-nsp14 E191A mutant-T20P14-R complex

Method: ELECTRON MICROSCOPY D_max: 150.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9q1j
deposition_date	2025-08-14
title	Cryo-EM structure of SARS-CoV-2 nsp10-nsp14 E191A mutant-T20P14-R complex
keywords	SARS-CoV-2, replication and transcription, mismatch, proofreading exoribonuclease, VIRAL PROTEIN, VIRAL PROTEIN-RNA complex; VIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	47.62 Å
rg_electron	47.38 Å
i0	1517750000.00
molecular_weight	307450.0 kDa
excluded_volume	376970 Å³
envelope_volume	541310 Å³
shell_volume	92469 Å³
envelope_diameter	162.1 Å
shell_rg	53.77 Å
envelope_rg	46.69 Å
shape_rg	47.34 Å
total_rg	47.76 Å
total_atoms	21410
n_residues	2628
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	150.2 Å
rg_real	47.34 Å
rg_real_error	1.11 Å
i0_real	1.5180e+09
i0_real_error	2.7610e+07
rg_reciprocal	47.62 Å
i0_reciprocal	1518000000.0000
total_estimate	0.8881
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	59.2 Å
skewness	0.139
kurtosis	-0.453
angular_range	— – 0.1650 Å⁻¹
current_alpha	0.0000
highest_alpha	99610000.0000
n_real_points	34
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.916; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.969; Smooth: 0.823

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	Non-structural protein 10
formula_weight	14802.9 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,C,G,I
atom_count	21410

AGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQ

Source

organism	Severe acute respiratory syndrome coronavirus 2
common_name	2019-nCoV, SARS-CoV-2
ncbi_taxonomy_id	2697049
host_organism	Escherichia coli

Entity 2 polymer

description	Proofreading exoribonuclease
formula_weight	59829.4 kDa
ec_number	3.1.13.-
mutation	E191A
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	B,D,H,J

AENVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFALTSMKYFVKIGPERTCCLCDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDLYCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVVKAALLADKFPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNVDRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMISAGFSLWVYKQFDTYNLWNTFTRLQ

Source

organism	Severe acute respiratory syndrome coronavirus 2
common_name	2019-nCoV, SARS-CoV-2
ncbi_taxonomy_id	2697049
host_organism	Escherichia coli

Entity 3 polymer

description	T20P14-R RNA
formula_weight	12541.5 kDa
src_method	syn
entity_count	2

Polymer

type	polyribonucleotide
strand_id	E,K

GGGAAUGGGAUUUUAAUAGCUUCGGCUAUUAAAAUCCCX

Entity 4 polymer

description	;RNA (5'-R(AUPUPCPCPCP*C)-3') ;
formula_weight	2117.3 kDa
src_method	syn
entity_count	2

Polymer

type	polyribonucleotide
strand_id	F,L

AUUCCCC

Entity 5 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	20

Entity 6 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	4

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.81 Å
resolution_method	—
num_particles	102206

fit	/app/data/saxs_computation/q1/9q1j/9q1j.fit
pddf	/app/data/pr_computation/q1/9q1j/9q1j.pr.out
profile	/app/data/saxs_computation/q1/9q1j/9q1j.dat

Cryo-EM structure of SARS-CoV-2 nsp10-nsp14 E191A mutant-T20P14-R complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Entity 4 polymer

Polymer

Entity 5 non-polymer

Entity 6 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)