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9qew

Cryo-EM structure of the undecorated actin filament in the ADP-Pi state.

Method: ELECTRON MICROSCOPY D_max: 171.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qew
deposition_date	2025-03-11
title	Cryo-EM structure of the undecorated actin filament in the ADP-Pi state.
keywords	actin, filament, cytoskeleton, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	46.42 Å
rg_electron	46.98 Å
i0	654360000.00
molecular_weight	208340.0 kDa
excluded_volume	259490 Å³
envelope_volume	336780 Å³
shell_volume	63546 Å³
envelope_diameter	184.8 Å
shell_rg	47.04 Å
envelope_rg	47.06 Å
shape_rg	47.00 Å
total_rg	46.94 Å
total_atoms	14590
n_residues	1845
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	171.6 Å
rg_real	46.95 Å
rg_real_error	1.80 Å
i0_real	6.5440e+08
i0_real_error	1.1990e+07
rg_reciprocal	46.42 Å
i0_reciprocal	653900000.0000
total_estimate	0.8135
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	45.4 Å
skewness	0.621
kurtosis	-0.094
angular_range	— – 0.1700 Å⁻¹
current_alpha	0.0000
highest_alpha	54590000.0000
n_real_points	35
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.598; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.899; Smooth: 0.878

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Actin, cytoplasmic 1, N-terminally processed
formula_weight	41632.4 kDa
mutation	C272A
src_method	man
entity_count	5

Polymer

type	polypeptide(L)
strand_id	C,A,B,D,E
atom_count	15280

DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Trichoplusia ni

Entity 2 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	5

Entity 3 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	5

Entity 4 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	syn
entity_count	5

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	690

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.18 Å
resolution_method	—
num_particles	4674353

fit	/app/data/saxs_computation/qe/9qew/9qew.fit
pddf	/app/data/pr_computation/qe/9qew/9qew.pr.out
profile	/app/data/saxs_computation/qe/9qew/9qew.dat