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9qs0

Crystal Structure of the MAR1-FUS1 Gamete Adhesion Complex in Chlamydomonas reinhardtii

Method: X-RAY DIFFRACTION D_max: 164.5 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qs0
deposition_date	2025-04-04
last_revision	2026-06-10
title	Crystal Structure of the MAR1-FUS1 Gamete Adhesion Complex in Chlamydomonas reinhardtii
keywords	Sexual reproduction, gamete adhesion, membrane fusion, HAP2/GCS1, Chlamydomonas, CELL ADHESION; CELL ADHESION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	44.14 Å
rg_electron	45.60 Å
i0	223800000.00
molecular_weight	121210.0 kDa
excluded_volume	151410 Å³
envelope_volume	213880 Å³
shell_volume	45504 Å³
envelope_diameter	176.8 Å
shell_rg	41.88 Å
envelope_rg	46.61 Å
shape_rg	45.61 Å
total_rg	45.36 Å
total_atoms	8526
n_residues	1095
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	164.5 Å
rg_real	44.89 Å
rg_real_error	1.89 Å
i0_real	2.2380e+08
i0_real_error	4.5020e+06
rg_reciprocal	44.15 Å
i0_reciprocal	223600000.0000
total_estimate	0.5196
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	45.7 Å
skewness	0.761
kurtosis	0.104
angular_range	— – 0.1800 Å⁻¹
current_alpha	0.0000
highest_alpha	14430000.0000
n_real_points	37
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.482; Stabil: 1.000; Sysdev: 0.005; Positv: 1.000; Valcen: 0.610; Smooth: 0.679

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	FUS1p
formula_weight	51590.7 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	8532

QDCSVVADFKIDFQTSIFIAGNAYNITLTLLDSYGDPTCVLYDPLLSVSCPSSSSNGRDFCSLQLSPLYNGVYNIKVIPQTLWGGHTVWPPPYSPVAPLPDVYFAGDTAIRITYNGRDIQGSPFPVTVQAEPHISTALSTVNVAVPAKGVASSRFAIAYFYQISDRFTNWIREKSIATQLRVSAYPDADISVQWWQNWWIVLYANSTSAGMYRFQVYFIDDDGSEVPIRILSPGLGLSYDGSFEVLPLALDEAKITASGLPQEVEAGIPVSLTLQAMDIYSNPTRLVDPESYQPFGQQPDNKTLLQVRLVTVDSGALQPNVVAIPTNTTGACSWSITFFTSMDYSVSVTYKESVLHMFSITVRNAQASPSNSTALLPEIGQAGTTLLYVTPRDLWGNIAPLANNDLSIGLTGSTFFHSFIPVEPVRKGDYSVYSLTLTEAGLYVVSIQLHNSSWLEKNITIEAGHHHHHH

Source

organism	Chlamydomonas reinhardtii
ncbi_taxonomy_id	3055
host_organism	Drosophila melanogaster

Entity 2 polymer

description	Minus adhesion receptor 1
formula_weight	17531.1 kDa
mutation	N56Q, N54Q, N72Q, N75Q
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,D

QVTLCNKFDSVAGQAQFDQACWNKAPCGGAQVTIPTAWQYSSPCTSQTSQTTSSCVPLPPGYASCADWQAVTNASPAPCRTLTCCASPYGVSYSRYNCAPANTTCLDTGPNSVACMPTQACPPGYIPDASGSCAGADDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK

Source

organism	Chlamydomonas reinhardtii
ncbi_taxonomy_id	3055
host_organism	Drosophila melanogaster

Entity 3 branched

description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	424.4 kDa
src_method	man
entity_count	2

Entity 4 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	syn
entity_count	9

Entity 5 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	9

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	6

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6. Citations (1)

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length_a	348.395 Å
length_b	89.684 Å
length_c	97.327 Å
angle_alpha	90.00 °
angle_beta	98.66 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 1 2 1
int_tables_number	5
crystal_system	monoclinic

resolution	3.100 Å
resolution_low	45.240 Å
r_factor	0.2179
r_work	0.2165
r_free	0.2459

fit	/app/data/saxs_computation/qs/9qs0/9qs0.fit
pddf	/app/data/pr_computation/qs/9qs0/9qs0.pr.out
profile	/app/data/saxs_computation/qs/9qs0/9qs0.dat

Crystal Structure of the MAR1-FUS1 Gamete Adhesion Complex in Chlamydomonas reinhardtii

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 branched

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)