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9r23

Cryo-EM structure of the double mutant H84V/E120G of the flotillin-associated rhodopsin PsFAR in detergent micelle

Method: ELECTRON MICROSCOPY D_max: 91.2 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9r23
deposition_date	2025-04-29
title	Cryo-EM structure of the double mutant H84V/E120G of the flotillin-associated rhodopsin PsFAR in detergent micelle
keywords	proton transport, retinal, bioenergetics, microbial rhodopsin, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	31.74 Å
rg_electron	30.56 Å
i0	199402000.00
molecular_weight	140920.0 kDa
excluded_volume	188410 Å³
envelope_volume	209720 Å³
shell_volume	53524 Å³
envelope_diameter	94.8 Å
shell_rg	40.26 Å
envelope_rg	30.60 Å
shape_rg	30.54 Å
total_rg	31.61 Å
total_atoms	9950
n_residues	1175
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	91.2 Å
rg_real	31.45 Å
rg_real_error	0.39 Å
i0_real	1.9940e+08
i0_real_error	2.8480e+06
rg_reciprocal	31.57 Å
i0_reciprocal	199400000.0000
total_estimate	0.9036
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	43.8 Å
skewness	0.010
kurtosis	-0.515
angular_range	— – 0.2500 Å⁻¹
current_alpha	0.0000
highest_alpha	34820000.0000
n_real_points	51
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.947; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.929

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	flotillin-associated rhodopsin
formula_weight	27407.2 kDa
src_method	man
entity_count	5

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E
atom_count	10039

MNSTLLPTDIVGGTFWLLSMALIGASIFFLLERNRVDGRWHTTMTLLGVTMLISAIFYYYVQGMWVDTGKAPIVLRYLDWILTVSMQVVMFYVILTAVTKVSSALFWRLLIGALVMVIGGFLGAAGYMSATLGFIIGVVGWLYILGEIYMGEASRCNIESGNEATHMAFNGLRLILTIGWAIYPLGYFINNLGGGVDANSLNIIYNLTDFLNKIIFGFVVYRAAMNDTQARLDEIKKLEHHHHHH

Source

organism	Candidatus Pseudothioglobus sp.
ncbi_taxonomy_id	3072909
host_organism	Escherichia coli

Entity 2 non-polymer

description	EICOSANE
formula_weight	282.5 kDa
src_method	syn
entity_count	66

Entity 3 non-polymer

description	RETINAL
formula_weight	284.4 kDa
src_method	man
entity_count	5

Source

organism	Candidatus Pseudothioglobus sp.
ncbi_taxonomy_id	3072909
host_organism	Escherichia coli

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	45

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.810 Å
resolution_low	107.010 Å
r_factor	0.4220
r_work	0.4220
r_free	—

resolution	2.81 Å
resolution_method	—
num_particles	116149

fit	/app/data/saxs_computation/r2/9r23/9r23.fit
pddf	/app/data/pr_computation/r2/9r23/9r23.pr.out
profile	/app/data/saxs_computation/r2/9r23/9r23.dat

Cryo-EM structure of the double mutant H84V/E120G of the flotillin-associated rhodopsin PsFAR in detergent micelle

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

Entity 3 non-polymer

Source

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)