SAXSdb — 9rst

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1. Structure Basics

entry_id	9rst
deposition_date	2025-07-01
title	Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) in autoinhibted conformation (monomer)
keywords	Cell growth, metabolism, SIGNALING PROTEIN; SIGNALING PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	69.05 Å
rg_electron	69.32 Å
i0	3015420000.00
molecular_weight	476870.0 kDa
excluded_volume	602700 Å³
envelope_volume	985310 Å³
shell_volume	121620 Å³
envelope_diameter	238.0 Å
shell_rg	66.12 Å
envelope_rg	67.35 Å
shape_rg	69.29 Å
total_rg	69.36 Å
total_atoms	33546
n_residues	4160
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	245.4 Å
rg_real	69.21 Å
rg_real_error	2.93 Å
i0_real	3.0150e+09
i0_real_error	6.6970e+07
rg_reciprocal	68.38 Å
i0_reciprocal	3011000000.0000
total_estimate	0.8472
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	74.7 Å
skewness	0.399
kurtosis	-0.427
angular_range	— – 0.1150 Å⁻¹
current_alpha	0.0000
highest_alpha	164100000.0000
n_real_points	24
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.806; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.972; Smooth: 0.620

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4. Crystallography & Experiment

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5. Entities & Polymer Info (7)

Entity 1 polymer

description	Serine/threonine-protein kinase TOR2
formula_weight	281915.4 kDa
ec_number	2.7.1.67,2.7.11.1
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	33546

MNKYINKYTTPPNLLSLRQRAEGKHRTRKKLTHKSHSHDDEMSTTSNTDSNHNGPNDSGRVITGSAGHIGKISFVDSELDTTFSTLNLIFDKLKSDVPQERASGANELSTTLTSLAREVSAEQFQRFSNSLNNKIFELIHGFTSSEKIGGILAVDTLISFYLSTEELPNQTSRLANYLRVLIPSSDIEVMRLAANTLGRLTVPGGTLTSDFVEFEVRTCIDWLTLTADNNSSSSKLEYRRHAALLIIKALADNSPYLLYPYVNSILDNIWVPLRDAKLIIRLDAAVALGKCLTIIQDRDPALGKQWFQRLFQGCTHGLSLNTNDSVHATLLVFRELLSLKAPYLRDKYDDIYKSTMKYKEYKFDVIRREVYAILPLLAAFDPAIFTKKYLDRIMVHYLRYLKNIDMNAANNSDKPFILVSIGDIAFEVGSSISPYMTLILDNIREGLRTKFKVRKQFEKDLFYCIGKLACALGPAFAKHLNKDLLNLMLNCPMSDHMQETLMILNEKIPSLESTVNSRILNLLSISLSGEKFIQSNQYDFNNQFSIEKARKSRNQSFMKKTGESNDDITDAQILIQCFKMLQLIHHQYSLTEFVRLITISYIEHEDSSVRKLAALTSCDLFIKDDICKQTSVHALHSVSEVLSKLLMIAITDPVAEIRLEILQHLGSNFDPQLAQPDNLRLLFMALNDEIFGIQLEAIKIIGRLSSVNPAYVVPSLRKTLLELLTQLKFSNMPKKKEESATLLCTLINSSDEVAKPYIDPILDVILPKCQDASSAVASTALKVLGELSVVGGKEMTRYLKELMPLIINTFQDQSNSFKRDAALTTLGQLAASSGYVVGPLLDYPELLGILINILKTENNPHIRRGTVRLIGILGALDPYKHREIEVTSNSKSSVEQNAPSIDIALLMQGVSPSNDEYYPTVVIHNLMKILNDPSLSIHHTAAIQAIMHIFQNLGLRCVSFLDQIIPGIILVMRSCPPSQLDFYFQQLGSLISIVKQHIRPHVEKIYGVIREFFPIIKLQITIISVIESISKALEGEFKRFVPETLTFFLDILENDQSNKRIVPIRILKSLVTFGPNLEDYSHLIMPIVVRMTEYSAGSLKKISIITLGRLAKNINLSEMSSRIVQALVRILNNGDRELTKATMNTLSLLLLQLGTDFVVFVPVINKALLRNRIQHSVYDQLVNKLLNNECLPTNIIFDKENEVPERKNYEDEMQVTKLPVNQNILKNAWYCSQQKTKEDWQEWIRRLSIQLLKESPSACLRSCSSLVSVYYPLARELFNASFSSCWVELQTSYQEDLIQALCKALSSSENPPEIYQMLLNLVEFMEHDDKPLPIPIHTLGKYAQKCHAFAKALHYKEVEFLEEPKNSTIEALISINNQLHQTDSAIGILKHAQQHNELQLKETWYEKLQRWEDALAAYNEKEAAGEDSVEVMMGKLRSLYALGEWEELSKLASEKWGTAKPEVKKAMAPLAAGAAWGLEQWDEIAQYTSVMKSQSPDKEFYDAILCLHRNNFKKAEVHIFNARDLLVTELSALVNESYNRAYNVVVRAQIIAELEEIIKYKKLPQNSDKRLTMRETWNTRLLGCQKNIDVWQRILRVRSLVIKPKEDAQVRIKFANLCRKSGRMALAKKVLNTLLEETDDPDHPNTAKASPPVVYAQLKYLWATGLQDEALKQLINFTSRMAHDLGLDPNNMIAQSVPQQSKRVPRHVEDYTKLLARCFLKQGEWRVCLQPKWRLSNPDSILGSYLLATHFDNTWYKAWHNWALANFEVISMLTSVSKKKQEGSDASSVTDINEFDNGMIGVNTFDAKEVHYSSNLIHRHVIPAIKGFFHSISLSESSSLQDALRLLTLWFTFGGIPEATQAMHEGFNLIQIGTWLEVLPQLISRIHQPNQIVSRSLLSLLSDLGKAHPQALVYPLMVAIKSESLSRQKAALSIIEKMRIHSPVLVDQAELVSHELIRMAVLWHEQWYEGLDDASRQFFGEHNTEKMFAALEPLYEMLKRGPETLREISFQNSFGRDLNDAYEWLMNYKKSKDVSNLNQAWDIYYNVFRKIGKQLPQLQTLELQHVSPKLLSAHDLELAVPGTRASGGKPIVKISKFEPVFSVISSKQRPRKFCIKGSDGKDYKYVLKGHEDIRQDSLVMQLFGLVNTLLQNDAECFRRHLDIQQYPAIPLSPKSGLLGWVPNSDTFHVLIREHREAKKIPLNIEHWVMLQMAPDYDNLTLLQKVEVFTYALNNTEGQDLYKVLWLKSRSSETWLERRTTYTRSLAVMSMTGYILGLGDRHPSNLMLDRITGKVIHIDFGDCFEAAILREKFPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMKVLRDNKGSLMAILEAFAFDPLINWGFDLPTKKIEEETGIQLPVMNANELLSNGAITEEEVQRVENEHKNAIRNARAMLVLKRITDKLTGNDIRRFNDLDVPEQVDKLIQQATSVENLCQHYIGWCPFW

Entity 2 polymer

description	Target of rapamycin complex subunit LST8
formula_weight	34077.9 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MSVILVSAGYDHTIRFWEALTGVCSRTIQHSDSQVNRLEITNDKKLLATAGHQNVRLYDIRTTNPNPVASFEGHRGNVTSVSFQQDNRWMVTSSEDGTIKVWDVRSPSIPRNYKHNAPVNEVVIHPNQGELISCDRDGNIRIWDLGENQCTHQLTPEDDTSLQSLSMASDGSMLAAANTKGNCYVWEMPNHTDASHLKPVTKFRAHSTYITRILLSSDVKHLATCSADHTARVWSIDDDFKLETTLDGHQRWVWDCAFSADSAYLVTASSDHYVRLWDLSTREIVRQYGGHHKGAVCVALNDV

Entity 3 polymer

description	Target of rapamycin complex 2 subunit TSC11
formula_weight	164580.6 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,H

MSIPHSAKQSSPLSSRRRSVTNTTPLLTPRHSRDNSSTQISSAKNITSSSPSTITNESSKRNKQNLVLSTSFISTKRLENSAPSPTSPLMARRTRSTMTKALLNLKAEINNQYQELARLRKKKDDIEHLRDSTISDIYSGSYSTNHLQKHSMRIRANTQLREIDNSIKRVEKHIFDLKQQFDKKRQRSLTTSSSIKADVGSIRNDDGQNNDSEELGDHDSLTDQVTLDDEYLTTPTSGTERNSQQNLNRNSTVNSRNNENHSTLSIPDLDGSNKVNLTGDTEKDLGDLENENQIFTSTTTEAATWLVSDYMQSFQEKNVNPDFIAQKANGLVTLLKEHSEIRKDLVLTSFMSSIQNLLLNGNKLIAASAYRVCRYLINSSIFIDELLELRLDAFIIISLAKDNSFQIEREQALKMVRRFIEYNNGVTQGIMQAIISCVEKPEDSLRHMALETLLELCFVAPEMVKECRGMRVIEGFLQDYTSFSLASVILDTILQLMATHKTRQHFLEDFNVSVLTTVFSDTNTKSNVNVEKMQNASTLISITLNSYNGFMLFSNNNFKPLKQLVSFFQIPICAQYLIDIFLDVLKIKPLPYKPRGRHSHSFKPIPSQYYKECMSVNQRLALIVLILENSEFVPHLLELLNEEDRDDHLVAKGRYLLTEYFNLRMNLVDKKYTSVSKPIYKENFTYVNETFQFKKIAYKMNRNRNTIGMSGIDYAQNIKSFSKNIKENTLLREVDDFRFRRMVYDSKVLQTKDFTRWNWNIINELLEGPLLNKKQLEELVKSTKFIRRLLVFYRPLRLRFSNVNKGAKLSQKYVQVGCQFFKTLTATPEGMKILMDDTKIIPQLASLMFRAMEGNISGNIFNKNKLREKIIFGYFKFIGILTQSKNGVHILTRWNFFTVIYKMFQFESKLGLEFLLLTIPELDLKYSSHCRVIIGKALVVANEKVRIEATKHIGDKLKELLSTKESDLKLKANKVKLQQFKMEMLTRQLYDLSPSVVAVADQALYECIVAGNGSEELGTSFRMFLNQMVFIRSPILFELLSRPYGFQLLNEINFVKEERDSWLSKKNIEYVHIVEEFLKKNESINAKSLTFQQKSRLPLHFYESLTKTEDGILLLSQTGDLVTFMNVIKKYVNGNNMATVENAKEILDLKAALWCVGFIGSTELGIGLLDNYSLVEDIIEVAYNASVTSVRFTAFYVLGLISMTREGCEILDEMGWNCCVSVQDEPIGIALPNRLDRFLSYNEHKWSAFGEYSDEMIVFNKSDGDLIEKCLPIEFDLDKLLKEKDTAENPLNEKIITNKYDNDITSQTITVSGENSSLFANEGLSSPYVTQYRNDDDSIESKVLHIVSQLGNHILSNHAVKEITEINNKYGPRLFENEKMFFKVFNMMSKYRFKPHVRKFLCGLFINNRALENVIRHDNKRDKRPANFTR

Entity 4 polymer

description	Target of rapamycin complex 2 subunit AVO2
formula_weight	47206.5 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MLKEPSVRLREAIIEGNLLIVKRLLRRNPDLLTNIDSENGWSSLHYASYHGRYLICVYLIQLGHDKHELIKTFKGNTCVHLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLLNEGEVSLDDNVRDKGNWKPIDVAQTFEVGNIYSKVLKEVKKKGPPLGAGKKPSSFRTPILNAKATFEDGPSPVLSMNSPYSLYSNNSPLPVLPRRISTHTTSGNGGNRRSSITNPVFNPRKPTLSTDSFSSSSNSSSRLRVNSINVKTPVGVSPKKELVSESVRHSATPTSPHNNIALINRYLLPNKSNDNVRGDSQTATINDDGGGGNGGDATIGMGLRKDPDDENENKYKIKVNNGEPRRRVSLLNIPISKLRNSNNTRAED

Entity 5 polymer

description	Target of rapamycin complex 2 subunit BIT61
formula_weight	60908.7 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E

MTAEDILLRERTSTTTQRPVNSEQYLNVQLATAPVKNFQTTSEISRQTLVDTSNDDVYSIKNLKGSRNPISPSVSNVGFQSIFHTVDHPRSKVSVASNHSLRSNDNASAATSKSGSSQIGESHSVDTVECSNNLSKKLSSDAISITQKSLHSTPSGRYMKGKASGFFNRRNRAHTTISSDPASFLTDSSTLHNSSHSFRNVIKNFFQNKSHRHIGQDAIEPAIPNSLSKFLHSSYGRHKSPSQFIHTNAGQLVDSGTSVYSLNVNPSGVNPNTIVEDPLSGTDPASPNPVSMLHDLLRNLPSLEANYKHFNSQELTTLTNNIWNIFCSNVAELFRTQRIWKLRAKIENFNEVLEFYCILKTDPRVTHSGMNRIISDLKEFLVSSLYNLENQIVFNYSNEDTINNALKRLGVIWRIFYQEVYYDLAAVLLPLDQSIREDGNSTVLKSGNESRTHINGNYSIGFLLLMCFRDSIVLPCYENFVNSNDGISKSFQLYIFNQEEESNVTETDKLTLLQCFGILSTIQSNDRNQRIIEELLAGIRMSI

Entity 6 polymer

description	Target of rapamycin complex 2 subunit AVO1
formula_weight	131565.5 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	G

MDTVTVLNELRAQFLRVCPEKDQMKRIIKPYIPVDEFNTEQCLDSSIRELYMNSDGVSLLPELESPPVSKDFMENYASLGKMRIMRENEGQKGKANQNLIGAEKTERDEEETRNLQDKSAKNTLIVEENGTLRYNPLNSSASNSLLNDDDHTSGKHHKTSSKEDSYLNSSMEMQKKSSKRSSLPFVRIFKSRRDHSNTSGNKNVMNTTNTRAKSSTLHPPGARHNKKGSKFDMNFDFDENLEEEDDDDDDDEEGDDIHSQFFQLDDDFDAKGSGASPAHKGINGMSNNKNNTYTNNRNSISILDDRESSNGNIGSASRLKSHFPTSQKGKIFLTDNKNDGQKSDSLNANKGIHGDGSSASGNGSVSRDGLTETESNNISDMESYINEKDLDDLNFDTVTSNINKTVSDLGGHESTNDGTAVMNRDSKDSRSNSNEFNAQNRDRITPGSSYGKSLLGSEYSEERYSNNDSSTMESGEMSLDSDMQTNTIPSHSIPMSMQKYGIYHGDDDSTLNNVFDKAVLTMNSSRHPKERRDTVISGKEPTSLTSSNRKFSVSSNLTSTRSPLLRGHGRTSSTASSEHMKAPKVSDSVLHRARKSTLTLKQDHSQPSVPSSVHKSSKEGNILIEKTTDYLVSKPKASQLSNMFNKKKKRTNTNSVDVLEYFSFVCGDKVPNYESMGLEIYIQASKKYKRNSFTTKVRKSSTIFEVIGFALFLYSTEKKPDNFEEDGLTVEDISNPNNFSLKIVDEDGEPFEDNFGKLDRKSTIQSISDSEVVLCKVDDAEKSQNEIETPLPFETGGGLMDASTLDANSSHDTTDGTINQLSFYKPIIGNEDDIDKTNGSKIIDVTVYLYPNVNPKFNYTTISVLVTSHINDILVKYCKMKNMDPNEYALKVLGKNYILDLNDTVLRLDGINKVELISKKDARELHLEKMKPDLKKPVLPTIQSNDLTPLTLEPLNSYLKADAGGAVAAIPENTKVTSKAKKISTKYKLGLAKQHSSSSVASGSVSTAGGLANGNGFFKNKNSSKSSLHGTLQFHNINRSQSTMEHTPDTPNGVGDNFQDLFTGAYHKYKVWRRQQMSFINKHERTLAIDGDYIYIVPPEGRIHWHDNVKTKSLHISQVVLVKKSKRVPEHFKIFVRREGQDDIKRYYFEAVSGQECTEIVTRLQNLLSAYRMNHK

Entity 7 non-polymer

description	INOSITOL HEXAKISPHOSPHATE
formula_weight	660.0 kDa
src_method	syn
entity_count	1

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.930 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.93 Å
resolution_method	—
num_particles	125785

fit	/app/data/saxs_computation/rs/9rst/9rst.fit
pddf	/app/data/pr_computation/rs/9rst/9rst.pr.out
profile	/app/data/saxs_computation/rs/9rst/9rst.dat

Cryo-EM structure of the Target of Rapamycin Complex 2 (TORC2) in autoinhibted conformation (monomer)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (7)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 polymer

Polymer

Entity 5 polymer

Polymer

Entity 6 polymer

Polymer

Entity 7 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)