← Back to search

9spn

p53 cancer mutant V157F in complex with DARPin C10

Method: X-RAY DIFFRACTION D_max: 70.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9spn
deposition_date	2025-09-17
last_revision	2026-04-22
title	p53 cancer mutant V157F in complex with DARPin C10
keywords	;p53, tumor suppressor, cancer mutation, protein stability, protein unfolding disease, designed ankyrin repeat proteins, thermostabilization, mutant p53 reactivation, cancer therapy, TRANSCRIPTION ;; TRANSCRIPTION
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	21.62 Å
rg_electron	20.58 Å
i0	22252300.00
molecular_weight	34318.0 kDa
excluded_volume	42252 Å³
envelope_volume	50175 Å³
shell_volume	20650 Å³
envelope_diameter	73.8 Å
shell_rg	26.78 Å
envelope_rg	20.70 Å
shape_rg	20.54 Å
total_rg	21.47 Å
total_atoms	2403
n_residues	319
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	70.7 Å
rg_real	21.60 Å
rg_real_error	0.51 Å
i0_real	2.2250e+07
i0_real_error	2.9790e+05
rg_reciprocal	21.61 Å
i0_reciprocal	22250000.0000
total_estimate	0.8145
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	24.9 Å
skewness	0.337
kurtosis	-0.362
angular_range	— – 0.3700 Å⁻¹
current_alpha	0.0000
highest_alpha	5305000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.868; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.983; Smooth: 0.000

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (5)

Entity 1 polymer

description	Cellular tumor antigen p53
formula_weight	22769.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2547

SSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRFRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGE

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 polymer

description	DARPin C10
formula_weight	13527.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E

GSDLGKKLLEAAWHGQDDEVRILMANGADVNATDQSGMTPLHLAAWRGHLEIVEVLLKTGADVNAIDRWGKTPLHLAARIGHLEIVEVLLKAGADVNAQDKFGKTPFDLAIDNGNEDIAEVLQKAA

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	Escherichia coli

Entity 3 non-polymer

description	1,2-ETHANEDIOL
formula_weight	62.1 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	137

▶

6. Citations (1)

▶

length_a	38.200 Å
length_b	92.896 Å
length_c	53.735 Å
angle_alpha	90.00 °
angle_beta	108.17 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	monoclinic

resolution	1.930 Å
resolution_low	46.450 Å
r_factor	0.1929
r_work	0.1913
r_free	0.2231

fit	/app/data/saxs_computation/sp/9spn/9spn.fit
pddf	/app/data/pr_computation/sp/9spn/9spn.pr.out
profile	/app/data/saxs_computation/sp/9spn/9spn.dat

p53 cancer mutant V157F in complex with DARPin C10

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)