p53 cancer mutant E285K in complex with DARPin C10-H82R

entry_id	9sps
deposition_date	2025-09-17
last_revision	2026-04-22
title	p53 cancer mutant E285K in complex with DARPin C10-H82R
keywords	;p53, tumor suppressor, cancer mutation, protein stability, protein unfolding disease, designed ankyrin repeat proteins, thermostabilization, mutant p53 reactivation, cancer therapy, TRANSCRIPTION ;; TRANSCRIPTION
method	X-RAY DIFFRACTION

rg_guinier	21.55 Å
rg_electron	20.60 Å
i0	23030600.00
molecular_weight	34986.0 kDa
excluded_volume	43139 ų
envelope_volume	51152 ų
shell_volume	21012 ų
envelope_diameter	73.9 Å
shell_rg	26.93 Å
envelope_rg	20.78 Å
shape_rg	20.57 Å
total_rg	21.48 Å
total_atoms	2447
n_residues	319
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	73.9 Å
rg_real	21.55 Å
rg_real_error	0.53 Å
i0_real	2.3030e+07
i0_real_error	3.3790e+05
rg_reciprocal	21.55 Å
i0_reciprocal	23030000.0000
total_estimate	0.8723
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	25.0 Å
skewness	0.366
kurtosis	-0.282
angular_range	— – 0.3700 Å−1
current_alpha	0.0000
highest_alpha	5344000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.794; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.959; Smooth: 0.994

Unit Cell

length_a	37.680 Å
length_b	93.911 Å
length_c	53.335 Å
angle_alpha	90.00 °
angle_beta	110.17 °
angle_gamma	90.00 °
z_pdb	2

Symmetry

space_group	P 1 21 1
int_tables_number	4
crystal_system	monoclinic

Refinement

resolution	1.420 Å
resolution_low	28.250 Å
r_factor	0.1474
r_work	0.1458
r_free	0.1774

Diffraction

temperature

100.0 K

Files (7)

• pr_distribution /app/data/pr_computation/sp/9sps/pr_distribution.json
• pr_output /app/data/pr_computation/sp/9sps/9sps.pr.out
• pr_plot /app/data/visualization/pr_computation/sp/9sps/9sps_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/sp/9sps/9sps.dat
• saxs_fit /app/data/saxs_computation/sp/9sps/9sps.fit
• saxs_log /app/data/saxs_computation/sp/9sps/9sps.log
• saxs_plot /app/data/visualization/saxs_computation/sp/9sps/9sps_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/sp/9sps/9sps.fit
pddf	/app/data/pr_computation/sp/9sps/9sps.pr.out
profile	/app/data/saxs_computation/sp/9sps/9sps.dat