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9tp8

Crystal structure of the C-terminal Ser/Thr phosphatase D510N H707K mutant of the Kelch phosphatase BSU1 from Arabidopsis thaliana

Method: X-RAY DIFFRACTION D_max: 75.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9tp8
deposition_date	2025-12-17
last_revision	2026-06-03
title	Crystal structure of the C-terminal Ser/Thr phosphatase D510N H707K mutant of the Kelch phosphatase BSU1 from Arabidopsis thaliana
keywords	protein phosphatase, ser/thr phosphatase, cell signaling, CELL CYCLE; CELL CYCLE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	20.17 Å
rg_electron	19.03 Å
i0	20404500.00
molecular_weight	35727.0 kDa
excluded_volume	45279 Å³
envelope_volume	50840 Å³
shell_volume	21739 Å³
envelope_diameter	77.2 Å
shell_rg	26.01 Å
envelope_rg	20.09 Å
shape_rg	19.01 Å
total_rg	20.06 Å
total_atoms	5044
n_residues	311
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	75.9 Å
rg_real	20.12 Å
rg_real_error	0.52 Å
i0_real	2.0400e+07
i0_real_error	2.6990e+05
rg_reciprocal	20.13 Å
i0_reciprocal	20400000.0000
total_estimate	0.8056
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	26.0 Å
skewness	0.381
kurtosis	0.228
angular_range	— – 0.3950 Å⁻¹
current_alpha	0.0000
highest_alpha	5105000.0000
n_real_points	71
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.504; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.977; Smooth: 0.981

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Serine/threonine-protein phosphatase BSU1
formula_weight	43644.8 kDa
ec_number	3.1.3.16
mutation	D510N,H707K
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5243

MEGYVPLQHGAETVGNRGGLVRTASLDQSTQDLHKKVISTLLRPKTWTPPANRDFFLSYLEVKHLCDEVEKIFMNEPTLLQLKVPIKVFGNIHGQYGDLMRLFHEYGHPSVEGDITHIDYLFLGDYVDRGQHSLEIIMLLFALKIEYPKNIHLIRGNHESLAMNRIYGFLTECEERMGESYGFEAWLKINQVFDYLPLAALLEKKVLCVHGGIGRAVTIEEIENIERPAFPDTGSMVLKDILWSDPTMNDTVLGIVDNARGEGVVSFGPDIVKAFLERNGLEMILRAKECVIDGFERFADGRLITVFSATNYCGTAQNAGAILVIGRDMVIYPKLIHPHPPPISSSEEDYTDKAWMQELNIEMPPTPARGESSEAAAENLYFQLNIE

Source

organism	Arabidopsis thaliana
common_name	thale cress
ncbi_taxonomy_id	3702
host_organism	Spodoptera frugiperda

Entity 2 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	5

Entity 3 non-polymer

description	ACETATE ION
formula_weight	59.0 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	139

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6. Citations (2)

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length_a	139.757 Å
length_b	44.047 Å
length_c	79.241 Å
angle_alpha	90.00 °
angle_beta	121.07 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	monoclinic

resolution	1.600 Å
resolution_low	41.340 Å
r_factor	0.1617
r_work	0.1606
r_free	0.1824

fit	/app/data/saxs_computation/tp/9tp8/9tp8.fit
pddf	/app/data/pr_computation/tp/9tp8/9tp8.pr.out
profile	/app/data/saxs_computation/tp/9tp8/9tp8.dat

Crystal structure of the C-terminal Ser/Thr phosphatase D510N H707K mutant of the Kelch phosphatase BSU1 from Arabidopsis thaliana

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction