Cryo-EM structure of the M1 muscarinic acetylcholine receptor bound to atropine and nanobody NbA12

Method: ELECTRON MICROSCOPY D_max: 87.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uaz
deposition_date	2025-04-02
title	Cryo-EM structure of the M1 muscarinic acetylcholine receptor bound to atropine and nanobody NbA12
keywords	GPCR, inactive-state, atropine, de novo protein, MEMBRANE PROTEIN, nanobody; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	25.83 Å
rg_electron	25.55 Å
i0	41376300.00
molecular_weight	34020.0 kDa
excluded_volume	33356 Å³
envelope_volume	59809 Å³
shell_volume	21173 Å³
envelope_diameter	90.2 Å
shell_rg	30.51 Å
envelope_rg	25.93 Å
shape_rg	25.56 Å
total_rg	26.01 Å
total_atoms	2591
n_residues	399
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	87.8 Å
rg_real	26.08 Å
rg_real_error	0.66 Å
i0_real	4.1380e+07
i0_real_error	6.1090e+05
rg_reciprocal	26.00 Å
i0_reciprocal	41370000.0000
total_estimate	0.8234
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	23.0 Å
skewness	0.567
kurtosis	-0.323
angular_range	— – 0.3050 Å⁻¹
current_alpha	0.0000
highest_alpha	7939000.0000
n_real_points	62
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.682; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.728; Smooth: 0.926

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.29 Å
resolution_method	—
num_particles	254687

description	M1 muscarinic acetylcholine receptor, de novo design protein
formula_weight	54691.6 kDa
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	B
atom_count	2591

Cryo-EM structure of the M1 muscarinic acetylcholine receptor bound to atropine and nanobody NbA12

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

description	[(1R,5S)-8-methyl-8-azabicyclo[3.2.1]octan-3-yl] (2S)-3-oxidanyl-2-phenyl-propanoate
formula_weight	289.4 kDa
src_method	syn
entity_count	1

fit	/app/data/saxs_computation/ua/9uaz/9uaz.fit
pddf	/app/data/pr_computation/ua/9uaz/9uaz.pr.out
profile	/app/data/saxs_computation/ua/9uaz/9uaz.dat