Cryo-EM structure of pyrene-modified TIP60 double mutant (G12C/S50C) with addition of Nile Red

Method: ELECTRON MICROSCOPY D_max: 242.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uol
deposition_date	2025-04-26
title	Cryo-EM structure of pyrene-modified TIP60 double mutant (G12C/S50C) with addition of Nile Red
keywords	Artificial designed protein complex, Fusion protein, Protein nanocage, Protein nanoparticle, Pyrene modification, DE NOVO PROTEIN; DE NOVO PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	93.18 Å
rg_electron	91.34 Å
i0	21567700000.00
molecular_weight	833080.0 kDa
excluded_volume	810180 Å³
envelope_volume	3447600 Å³
shell_volume	324020 Å³
envelope_diameter	221.9 Å
shell_rg	100.80 Å
envelope_rg	76.75 Å
shape_rg	91.30 Å
total_rg	91.51 Å
total_atoms	63180
n_residues	7380
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	242.4 Å
rg_real	91.36 Å
rg_real_error	0.44 Å
i0_real	2.1200e+10
i0_real_error	4.1540e+08
rg_reciprocal	98.01 Å
i0_reciprocal	21920000000.0000
total_estimate	0.7623
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	156.8 Å
skewness	-0.572
kurtosis	-0.651
angular_range	— – 0.0850 Å⁻¹
current_alpha	0.5958
highest_alpha	353600000000.0000
n_real_points	18
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.538; Stabil: 0.983; Sysdev: 1.000; Positv: 1.000; Valcen: 0.842; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (4)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.80 Å
resolution_method	—
num_particles	22050

description	TIP60 double mutant (G12C/S50C)
formula_weight	17856.4 kDa
mutation	G12C, S50C
src_method	man
entity_count	60

type	polypeptide(L)
strand_id	;A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P,Q,R,S,T,V,W,X,Y,Z,AA,BA,CA,DA,EA,FA,GA,HA,IA,JA,KA,LA,MA,NA,OA,PA,QA,RA,SA,TA,UA,VA,WA,XA,YA,ZA,AB,BB,CB,DB,EB,FB,GB,HB,IB ;
atom_count	63180

Cryo-EM structure of pyrene-modified TIP60 double mutant (G12C/S50C) with addition of Nile Red

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

6. Citations (4)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	Escherichia coli BL21(DE3)

description	N-(1-pyrenyl)maleimide
formula_weight	297.3 kDa
src_method	syn
entity_count	120

fit	/app/data/saxs_computation/uo/9uol/9uol.fit
pddf	/app/data/pr_computation/uo/9uol/9uol.pr.out
profile	/app/data/saxs_computation/uo/9uol/9uol.dat