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9uva

Structures of multiple states of the nicotinamide nucleotide transhydrogenase from Escherichia coli

Method: ELECTRON MICROSCOPY D_max: 120.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uva
deposition_date	2025-05-09
title	Structures of multiple states of the nicotinamide nucleotide transhydrogenase from Escherichia coli
keywords	;Nicotinamide nucleotide transhydrogenase, Mitochondrial inner membrane, E. coli transhydrogenase, Hydride transfer, Proton motive force, NAD+, NADH, NADP+, NADPH, MEMBRANE PROTEIN ;; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	43.30 Å
rg_electron	42.76 Å
i0	463125000.00
molecular_weight	187390.0 kDa
excluded_volume	238940 Å³
envelope_volume	334100 Å³
shell_volume	63095 Å³
envelope_diameter	131.1 Å
shell_rg	50.82 Å
envelope_rg	41.10 Å
shape_rg	42.78 Å
total_rg	43.08 Å
total_atoms	13177
n_residues	1763
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	120.3 Å
rg_real	43.09 Å
rg_real_error	0.84 Å
i0_real	4.6310e+08
i0_real_error	7.5950e+06
rg_reciprocal	43.30 Å
i0_reciprocal	463200000.0000
total_estimate	0.8461
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	56.8 Å
skewness	0.010
kurtosis	-0.723
angular_range	— – 0.1800 Å⁻¹
current_alpha	0.0000
highest_alpha	24340000.0000
n_real_points	37
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.998; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.003

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	NAD(P) transhydrogenase subunit beta
formula_weight	48762.7 kDa
ec_number	7.1.1.1
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,A
atom_count	13177

MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIALIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNRHKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL

Source

organism	Escherichia coli (strain K12)
ncbi_taxonomy_id	83333
host_organism	Escherichia coli

Entity 2 polymer

description	NAD(P) transhydrogenase subunit alpha
formula_weight	10742.8 kDa
ec_number	7.1.1.1
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,D

ILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFKN

Source

organism	Escherichia coli (strain K12)
ncbi_taxonomy_id	83333
host_organism	Escherichia coli

Entity 3 polymer

description	NAD(P) transhydrogenase subunit alpha
formula_weight	40183.9 kDa
ec_number	7.1.1.1
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	E,F

HHGRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQV

Source

organism	Escherichia coli (strain K12)
ncbi_taxonomy_id	83333
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.73 Å
resolution_method	—
num_particles	55221

fit	/app/data/saxs_computation/uv/9uva/9uva.fit
pddf	/app/data/pr_computation/uv/9uva/9uva.pr.out
profile	/app/data/saxs_computation/uv/9uva/9uva.dat

Structures of multiple states of the nicotinamide nucleotide transhydrogenase from Escherichia coli

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)