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9v48

Coupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex

Method: ELECTRON MICROSCOPY D_max: 93.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9v48
deposition_date	2025-05-22
title	Coupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex
keywords	Influenza virus, ribonucleoprotein complex, polymerase, cryo-EM, VIRAL PROTEIN/RNA, VIRAL PROTEIN-RNA complex; VIRAL PROTEIN/RNA
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.75 Å
rg_electron	27.29 Å
i0	83147900.00
molecular_weight	60157.0 kDa
excluded_volume	70583 Å³
envelope_volume	100570 Å³
shell_volume	31826 Å³
envelope_diameter	99.4 Å
shell_rg	33.30 Å
envelope_rg	27.34 Å
shape_rg	27.26 Å
total_rg	27.92 Å
total_atoms	4153
n_residues	460
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	93.6 Å
rg_real	28.81 Å
rg_real_error	0.70 Å
i0_real	8.3150e+07
i0_real_error	1.2400e+06
rg_reciprocal	28.79 Å
i0_reciprocal	83150000.0000
total_estimate	0.8532
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	34.0 Å
skewness	0.476
kurtosis	-0.062
angular_range	— – 0.2750 Å⁻¹
current_alpha	0.0000
highest_alpha	9119000.0000
n_real_points	56
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.842; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.986; Smooth: 0.574

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Nucleoprotein
formula_weight	56172.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D
atom_count	4153

MASQGTKRSYEQMETDGERQNATEIRASVGKMINGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNRYLEEHPSAGKDPKKTGGPIYKRVDGKWMRELVLYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINDRNFWRGENGRKTRSAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFSARSALILRGSVAHKSCLPACVYGPAVASGYDFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRLLSFIRGTKVSPRGKLSTRGVQIASNENMDTMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPAFSVQRNLPFDKSTIMAAFTGNTEGRTSDMRAEIIRMMEGAKPEEVSFRGRGVFELSDEKATNPVVPSFDMSNEGSYFFGDNAEEYDN

Source

organism	Influenza A virus (A/Victoria/3/1975(H3N2))
ncbi_taxonomy_id	392809
host_organism	Homo sapiens

Entity 2 polymer

description	;RNA (5'-R(PUPCPUPUPUPGPUPCPAPCPCPCPUPGPCPU)-3') ;
formula_weight	4948.9 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	E

UCUUUGUCACCCUGCU

Source

organism	Influenza A virus (A/Victoria/3/1975(H3N2))
ncbi_taxonomy_id	392809
host_organism	Homo sapiens

Entity 3 polymer

description	;RNA (5'-R(PAPGPUPAPGPAPAPAPCPAPAPGPGPGPUPGPUPUPUPUPUPUPAPU)-3') ;
formula_weight	7720.6 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	F

AGUAGAAACAAGGGUGUUUUUUAU

Source

organism	Influenza A virus (A/Victoria/3/1975(H3N2))
ncbi_taxonomy_id	392809
host_organism	Homo sapiens

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6. Citations (1)

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resolution	4.75 Å
resolution_method	—
num_particles	265200

fit	/app/data/saxs_computation/v4/9v48/9v48.fit
pddf	/app/data/pr_computation/v4/9v48/9v48.pr.out
profile	/app/data/saxs_computation/v4/9v48/9v48.dat

Coupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)