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9znb

Crystal Structure of a DARPin Fused to the 1TEL Crystallization Chaperone via a Direct Helical Fusion in a 3-Fold Crystal Form

Method: X-RAY DIFFRACTION D_max: 78.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9znb
deposition_date	2025-12-12
title	Crystal Structure of a DARPin Fused to the 1TEL Crystallization Chaperone via a Direct Helical Fusion in a 3-Fold Crystal Form
keywords	TELSAM, DARPin, ETV6, designed ankyrin repeat protein, PROTEIN BINDING; PROTEIN BINDING
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.85 Å
rg_electron	21.41 Å
i0	12216200.00
molecular_weight	25324.0 kDa
excluded_volume	31233 Å³
envelope_volume	37196 Å³
shell_volume	15974 Å³
envelope_diameter	78.8 Å
shell_rg	26.20 Å
envelope_rg	21.53 Å
shape_rg	21.40 Å
total_rg	22.11 Å
total_atoms	3407
n_residues	238
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	78.0 Å
rg_real	22.08 Å
rg_real_error	0.87 Å
i0_real	1.2220e+07
i0_real_error	2.1100e+05
rg_reciprocal	22.04 Å
i0_reciprocal	12220000.0000
total_estimate	0.8018
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	19.8 Å
skewness	0.545
kurtosis	-0.256
angular_range	— – 0.3650 Å⁻¹
current_alpha	0.0000
highest_alpha	3868000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.646; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.535; Smooth: 0.947

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Transcription factor ETV6,SAM Domain of Transcription factor ETV6 fused to a DARPin
formula_weight	27109.3 kDa
mutation	V112E in ETV6 (Uniprot numbering)
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3538

MGHHHHHHHHHHSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFEMNGKALLLLTKEDFRYRSPHSGDELYELLQHILLGRDLLEAARAGQDDEVRILMANGADVNATDNDGYTPLHLAASNGHLEIVEVLLKNGADVNASDLTGITPLHAAAATGHLEIVEVLLKHGADVNAYDNDGHTPLHLAAKYGHLEIVEVLLKHGADVNAQDKFGKTAFDISIDNGNEDLAEILQKKN

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	Escherichia coli B

Entity 2 non-polymer

description	ACETIC ACID
formula_weight	60.1 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	4

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	117

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6. Citations (1)

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length_a	83.286 Å
length_b	83.286 Å
length_c	58.431 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 65
int_tables_number	170
crystal_system	hexagonal

resolution	1.771 Å
resolution_low	41.640 Å
r_factor	0.1954
r_work	0.1942
r_free	0.2195

fit	/app/data/saxs_computation/zn/9znb/9znb.fit
pddf	/app/data/pr_computation/zn/9znb/9znb.pr.out
profile	/app/data/saxs_computation/zn/9znb/9znb.dat