{ "code": "SASDD22", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/A", "project": { "title": "Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.", "publication": { "title": "Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.", "author_list": "Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V", "journal": "J Mol Biol", "doi": "10.1016/j.jmb.2018.02.020", "pmid": "29501381", "published_date": "2018 Apr 13" }, "status": "released", "submitted_date": "2017-12-04", "released_date": "2018-03-11" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDD22.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDD22.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD22_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD22_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD22_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD22_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDD22.sascif", "experiment": { "instrument": { "detector": { "type": "CCD", "name": "AVIEX PCCD170170", "resolution": null }, "name": "SOLEIL", "city": "Saint-Aubin", "country": "France", "beamline_name": "SWING", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Calvin cycle protein CP12, chloroplastic", "short_name": "CP12", "sequence": "HHHHHHHHHHSSGHIEGRHMSGQPAVDLNKKVQDAVKEAEDACAKGTSADCAVAWDTVEELSAAVSHKKDAVKADVTLTDPLEAFCKDAPDADECRVYED", "organism": "Chlamydomonas reinhardtii", "uniprot_code": "A6Q0K5", "uniprot_range_first": 28, "uniprot_range_last": 107, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MMLTKSVVISRPAVRPVSTRRAVVVRASGQPAVDLNKKVQDAVKEAEDACAKGTSADCAV\nAWDTVEELSAAVSHKKDAVKADVTLTDPLEAFCKDAPDADECRVYED", "mw": 10.934, "total_mw": 10.934, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Glyceraldehyde-3-phosphate dehydrogenase", "short_name": "GAPDH", "sequence": "EKKIRVAINGFGRIGRNFLRCWHGRQNTLLDVVAINDSGGVKQASHLLKYDSTLGTFAADVKIVDDSHISVDGKQIKIVSSRDPLQLPWKEMNIDLVIEGTGVFIDKVGAGKHIQAGASKVLITAPAKDKDIPTFVVGVNEGDYKHEYPIISNASCTTNCLAPFVKVLEQKFGIVKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTTTGAAKAVSLVLPSLKGKLNGIALRVPTPTVSVVDLVVQVEKKTFAEEVNAAFREAANGPMKGVLHVEDAPLVSIDFKCTDQSTSIDASLTMVMGDDMVKVVAWYDNEWGYSQRVVDLAEVTAKKWVA", "organism": "Chlamydomonas reinhardtii", "uniprot_code": "A8HP84", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "tetramer", "molecular_type": "protein", "uniprot_sequence": "MAAMMQKSAFTGSAVSSKSGVRAKAARAVVDVRAEKKIRVAINGFGRIGRNFLRCWHGRQ\nNTLLDVVAINDSGGVKQASHLLKYDSTLGTFAADVKIVDDSHISVDGKQIKIVSSRDPLQ\nLPWKEMNIDLVIEGTGVFIDKVGAGKHIQAGASKVLITAPAKDKDIPTFVVGVNEGDYKH\nEYPIISNASCTTNCLAPFVKVLEQKFGIVKGTMTTTHSYTGDQRLLDASHRDLRRARAAA\nLNIVPTTTGAAKAVSLVLPSLKGKLNGIALRVPTPTVSVVDLVVQVEKKTFAEEVNAAFR\nEAANGPMKGVLHVEDAPLVSIDFKCTDQSTSIDASLTMVMGDDMVKVVAWYDNEWGYSQR\nVVDLAEVTAKKWVA", "mw": 36.881, "total_mw": 147.524, "number_molecules": 4, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.9, "deuteration": null }, "purity_method": null, "name": "Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Marseille, France", "full_name": "Aix Marseille Univ, CNRS, BIP UMR7281", "webpage": "http://bip.cnrs-mrs.fr" } ], "contributor_name": "Veronique", "contributor_surname": "Receveur-Brechot", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2010-12-08", "storage_temperature": 15.0, "cell_temperature": 20.0, "exposure_time": 1.0, "number_of_frames": 250, "wavelength": 0.1033, "sample_detector_distance": 1.832, "concentration_min": null, "concentration_max": 16.0, "sample_volume": null, "flow_rate": null, "s_min": 0.105, "s_max": 4.595, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD22_fit1_fixed_fit_img.png", "software": "EOM/GAJOE", "chi_square_value": 5.291, "p_value": 0.0, "fit_residual_plot": "fit4columns1223_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDD22_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_8Gj01w3.log", "software_version": null, "description": null }, { "models": [], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD22_fit2_fixed_fit_img.png", "software": "EOM/GAJOE", "chi_square_value": 29.721, "p_value": 0.0, "fit_residual_plot": "fit4columns1224_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDD22_fit2.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Size_distr_003_1.dat", "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 in 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH 7.9 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 16.00 mg/ml was measured at 20°C. 250 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.", "tags": [], "intensity_unit": "1/cm", "experimental_mw": 166.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 0.0003877, "pddf_i0_error": 2e-07, "guinier_i0": 0.0003852, "guinier_i0_error": 1e-08, "pddf_rg": 3.99, "pddf_rg_error": 0.006, "guinier_rg": 3.84, "guinier_rg_error": 0.01, "pddf_dmax": 19.0, "pddf_dmax_error": 1.0, "porod_volume": 250.82, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 31, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDD22_datloglog_img.png", "symmetry": null, "last_modified": "2020-08-28T15:27:27.968395+02:00", "bragg_peak": [] }