data_SASDF23_MAIN # _sas_result.id 1313 _sas_result.SASBDB_code SASDF23 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 15.4800000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 10.200 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 6 _sas_result.Guinier_point_max 18 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 320-324 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 320-324)" _sas_scan.detc_id 15 _sas_scan.id 1313 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1274 _sas_scan.result_id 1313 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1274 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 320-324)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1274 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 1.523200E+01 1.549549E+01 1313 2 5.968380E-03 9.016800E+00 7.703506E+00 1313 3 6.388220E-03 8.347400E+00 5.468126E+00 1313 4 6.808050E-03 1.393380E+01 3.739030E+00 1313 5 7.227880E-03 1.360800E+01 3.756184E+00 1313 6 7.647710E-03 1.344500E+01 3.170282E+00 1313 7 8.067540E-03 1.319750E+01 2.566300E+00 1313 8 8.487370E-03 1.183894E+01 2.304264E+00 1313 9 8.907200E-03 1.079824E+01 2.031132E+00 1313 10 9.327030E-03 1.127770E+01 1.768198E+00 1313 11 9.746860E-03 1.066614E+01 1.692186E+00 1313 12 1.016670E-02 1.145440E+01 1.553968E+00 1313 13 1.058650E-02 1.115068E+01 1.387110E+00 1313 14 1.100630E-02 9.820780E+00 1.415224E+00 1313 15 1.142620E-02 9.129580E+00 1.171474E+00 1313 16 1.184600E-02 8.922160E+00 1.128340E+00 1313 17 1.226580E-02 9.409720E+00 1.102016E+00 1313 18 1.268570E-02 9.220340E+00 9.937864E-01 1313 19 1.310550E-02 7.923340E+00 1.082684E+00 1313 20 1.352530E-02 8.002200E+00 9.229856E-01 1313 21 1.394520E-02 7.353500E+00 8.762196E-01 1313 22 1.436500E-02 7.503900E+00 8.877232E-01 1313 23 1.478480E-02 7.592480E+00 8.063246E-01 1313 24 1.520460E-02 7.154420E+00 8.001168E-01 1313 25 1.562450E-02 6.779740E+00 7.870590E-01 1313 26 1.604430E-02 6.566260E+00 7.014014E-01 1313 27 1.646410E-02 6.867160E+00 6.904614E-01 1313 28 1.688400E-02 5.824240E+00 6.333506E-01 1313 29 1.730380E-02 5.373760E+00 6.207894E-01 1313 30 1.772360E-02 5.585000E+00 5.984392E-01 1313 31 1.814350E-02 5.243800E+00 5.899204E-01 1313 32 1.856330E-02 5.946960E+00 5.806264E-01 1313 33 1.898310E-02 5.777540E+00 5.511152E-01 1313 34 1.940290E-02 5.070860E+00 5.337486E-01 1313 35 1.982280E-02 4.833760E+00 5.217434E-01 1313 36 2.024260E-02 3.873120E+00 5.240064E-01 1313 37 2.066240E-02 4.531400E+00 5.043258E-01 1313 38 2.108230E-02 4.500060E+00 4.879734E-01 1313 39 2.150210E-02 4.660940E+00 4.872904E-01 1313 40 2.192190E-02 4.154980E+00 4.784234E-01 1313 41 2.234180E-02 4.059960E+00 4.707068E-01 1313 42 2.276160E-02 3.951900E+00 4.458844E-01 1313 43 2.318140E-02 3.791100E+00 4.552272E-01 1313 44 2.360130E-02 3.636280E+00 4.438976E-01 1313 45 2.402110E-02 3.310100E+00 4.247932E-01 1313 46 2.444090E-02 3.770500E+00 4.240468E-01 1313 47 2.486070E-02 3.598740E+00 3.954230E-01 1313 48 2.528060E-02 3.320700E+00 4.132274E-01 1313 49 2.570040E-02 3.538380E+00 4.011138E-01 1313 50 2.612020E-02 3.247180E+00 3.935808E-01 1313 51 2.654010E-02 3.081180E+00 3.883552E-01 1313 52 2.695990E-02 2.829880E+00 3.784528E-01 1313 53 2.737970E-02 3.077900E+00 3.977204E-01 1313 54 2.779960E-02 2.693240E+00 3.804730E-01 1313 55 2.821940E-02 2.631120E+00 3.782186E-01 1313 56 2.863920E-02 2.765140E+00 3.772648E-01 1313 57 2.905900E-02 2.440660E+00 3.705414E-01 1313 58 2.947890E-02 2.451940E+00 3.810560E-01 1313 59 2.989870E-02 2.262720E+00 3.612208E-01 1313 60 3.031850E-02 2.140300E+00 3.596138E-01 1313 61 3.073840E-02 2.079340E+00 3.641788E-01 1313 62 3.115820E-02 2.070200E+00 3.690960E-01 1313 63 3.157800E-02 2.217580E+00 3.700602E-01 1313 64 3.199790E-02 2.202280E+00 3.434156E-01 1313 65 3.241770E-02 2.113800E+00 3.660318E-01 1313 66 3.283750E-02 1.926360E+00 3.487060E-01 1313 67 3.325730E-02 1.872600E+00 3.497534E-01 1313 68 3.367720E-02 1.708280E+00 3.540134E-01 1313 69 3.409700E-02 1.825740E+00 3.403142E-01 1313 70 3.451680E-02 1.986280E+00 3.580812E-01 1313 71 3.493670E-02 1.441180E+00 3.299794E-01 1313 72 3.535650E-02 1.647820E+00 3.445892E-01 1313 73 3.577630E-02 1.586560E+00 3.479268E-01 1313 74 3.619620E-02 1.681140E+00 3.380836E-01 1313 75 3.661600E-02 1.483280E+00 3.384436E-01 1313 76 3.703580E-02 1.435020E+00 3.247308E-01 1313 77 3.745560E-02 1.605500E+00 3.277938E-01 1313 78 3.787550E-02 1.523600E+00 3.248450E-01 1313 79 3.829530E-02 1.360360E+00 3.249864E-01 1313 80 3.871510E-02 1.365360E+00 3.086846E-01 1313 81 3.913500E-02 1.473640E+00 3.054332E-01 1313 82 3.955480E-02 1.408440E+00 3.091384E-01 1313 83 3.997460E-02 1.440640E+00 3.029156E-01 1313 84 4.039450E-02 1.495540E+00 3.012444E-01 1313 85 4.081430E-02 1.248000E+00 2.993634E-01 1313 86 4.123410E-02 1.196040E+00 2.897054E-01 1313 87 4.165390E-02 1.060640E+00 2.946886E-01 1313 88 4.207380E-02 1.075640E+00 2.816376E-01 1313 89 4.249360E-02 1.006680E+00 2.889470E-01 1313 90 4.291340E-02 9.290198E-01 2.858794E-01 1313 91 4.333330E-02 9.775600E-01 2.769442E-01 1313 92 4.375310E-02 9.389002E-01 2.763534E-01 1313 93 4.417290E-02 1.003880E+00 2.715730E-01 1313 94 4.459280E-02 9.156202E-01 2.739702E-01 1313 95 4.501260E-02 1.075340E+00 2.642472E-01 1313 96 4.543240E-02 8.870000E-01 2.665344E-01 1313 97 4.585220E-02 9.826200E-01 2.704914E-01 1313 98 4.627210E-02 9.206002E-01 2.623212E-01 1313 99 4.669190E-02 8.696400E-01 2.679758E-01 1313 100 4.711170E-02 8.121202E-01 2.604096E-01 1313 101 4.753160E-02 6.650200E-01 2.473408E-01 1313 102 4.795140E-02 7.618798E-01 2.583940E-01 1313 103 4.837120E-02 8.279596E-01 2.490774E-01 1313 104 4.879110E-02 4.960400E-01 2.572570E-01 1313 105 4.921090E-02 5.155600E-01 2.500502E-01 1313 106 4.963070E-02 6.600000E-01 2.565270E-01 1313 107 5.005050E-02 8.528202E-01 2.587266E-01 1313 108 5.047040E-02 9.104204E-01 2.555232E-01 1313 109 5.089020E-02 6.842600E-01 2.456912E-01 1313 110 5.131000E-02 9.223002E-01 2.477262E-01 1313 111 5.172990E-02 7.443196E-01 2.462378E-01 1313 112 5.214970E-02 6.186198E-01 2.394676E-01 1313 113 5.256950E-02 8.170808E-01 2.478932E-01 1313 114 5.298940E-02 5.264000E-01 2.350826E-01 1313 115 5.340920E-02 4.842197E-01 2.343136E-01 1313 116 5.382900E-02 6.437400E-01 2.438968E-01 1313 117 5.424890E-02 5.072196E-01 2.346088E-01 1313 118 5.466870E-02 6.319600E-01 2.353954E-01 1313 119 5.508850E-02 8.265600E-01 2.332284E-01 1313 120 5.550830E-02 6.332804E-01 2.351632E-01 1313 121 5.592820E-02 5.116796E-01 2.373834E-01 1313 122 5.634800E-02 6.964602E-01 2.312608E-01 1313 123 5.676780E-02 6.583000E-01 2.348496E-01 1313 124 5.718770E-02 4.218997E-01 2.265462E-01 1313 125 5.760750E-02 3.057401E-01 2.285258E-01 1313 126 5.802730E-02 5.057000E-01 2.265832E-01 1313 127 5.844720E-02 4.750000E-01 2.281680E-01 1313 128 5.886700E-02 5.452798E-01 2.302402E-01 1313 129 5.928680E-02 5.892398E-01 2.223888E-01 1313 130 5.970660E-02 6.972194E-01 2.274104E-01 1313 131 6.012650E-02 5.841798E-01 2.254208E-01 1313 132 6.054630E-02 4.040400E-01 2.232080E-01 1313 133 6.096610E-02 4.931600E-01 2.220894E-01 1313 134 6.138600E-02 3.548808E-01 2.174592E-01 1313 135 6.180580E-02 2.758402E-01 2.208510E-01 1313 136 6.222560E-02 5.453802E-01 2.184886E-01 1313 137 6.264550E-02 5.190594E-01 2.198506E-01 1313 138 6.306530E-02 5.436398E-01 2.169462E-01 1313 139 6.348510E-02 6.187600E-01 2.145104E-01 1313 140 6.390490E-02 3.765604E-01 2.164988E-01 1313 141 6.432480E-02 3.883200E-01 2.127016E-01 1313 142 6.474460E-02 4.861005E-01 2.143738E-01 1313 143 6.516440E-02 3.796600E-01 2.131964E-01 1313 144 6.558430E-02 4.491800E-01 2.128058E-01 1313 145 6.600410E-02 4.945200E-01 2.096874E-01 1313 146 6.642390E-02 6.542994E-01 2.087152E-01 1313 147 6.684380E-02 4.178400E-01 2.094384E-01 1313 148 6.726360E-02 2.111000E-01 2.062458E-01 1313 149 6.768340E-02 3.920600E-01 2.067298E-01 1313 150 6.810320E-02 5.006602E-01 2.048890E-01 1313 151 6.852310E-02 4.787600E-01 2.040712E-01 1313 152 6.894290E-02 4.109796E-01 2.049060E-01 1313 153 6.936270E-02 6.152206E-01 2.020412E-01 1313 154 6.978260E-02 3.516405E-01 2.040900E-01 1313 155 7.020240E-02 3.877600E-01 2.028380E-01 1313 156 7.062220E-02 3.071000E-01 2.030206E-01 1313 157 7.104210E-02 4.034200E-01 2.069866E-01 1313 158 7.146190E-02 3.567402E-01 2.003972E-01 1313 159 7.188170E-02 3.759799E-01 2.003730E-01 1313 160 7.230160E-02 2.504801E-01 1.953526E-01 1313 161 7.272140E-02 1.748797E-01 1.973266E-01 1313 162 7.314120E-02 3.244000E-01 1.947362E-01 1313 163 7.356100E-02 5.103004E-01 1.962300E-01 1313 164 7.398090E-02 3.632397E-01 1.989190E-01 1313 165 7.440070E-02 4.309802E-01 1.945390E-01 1313 166 7.482050E-02 3.789802E-01 1.932498E-01 1313 167 7.524040E-02 4.779604E-01 1.940870E-01 1313 168 7.566020E-02 3.771999E-01 1.927506E-01 1313 169 7.608000E-02 2.168999E-01 1.939888E-01 1313 170 7.649980E-02 5.575400E-01 1.916172E-01 1313 171 7.691970E-02 2.294600E-01 1.933264E-01 1313 172 7.733950E-02 2.713602E-01 1.926314E-01 1313 173 7.775930E-02 3.775000E-01 1.915416E-01 1313 174 7.817920E-02 3.069400E-01 1.933326E-01 1313 175 7.859900E-02 2.995604E-01 1.913428E-01 1313 176 7.901880E-02 2.288393E-01 1.943082E-01 1313 177 7.943870E-02 2.574799E-01 1.895718E-01 1313 178 7.985850E-02 3.375398E-01 1.900548E-01 1313 179 8.027830E-02 4.400802E-01 1.941358E-01 1313 180 8.069810E-02 2.670600E-01 1.880704E-01 1313 181 8.111800E-02 3.116006E-01 1.922070E-01 1313 182 8.153780E-02 2.580996E-01 1.886286E-01 1313 183 8.195760E-02 2.960000E-01 1.892830E-01 1313 184 8.237750E-02 2.137398E-01 1.885346E-01 1313 185 8.279730E-02 3.640998E-01 1.881716E-01 1313 186 8.321710E-02 1.347006E-01 1.888972E-01 1313 187 8.363700E-02 1.760795E-01 1.881840E-01 1313 188 8.405680E-02 1.243600E-01 1.925286E-01 1313 189 8.447660E-02 3.128400E-01 1.969794E-01 1313 190 8.489650E-02 3.148200E-01 1.992062E-01 1313 191 8.531630E-02 2.448997E-01 1.984676E-01 1313 192 8.573610E-02 1.246405E-01 1.954542E-01 1313 193 8.615590E-02 2.517200E-01 2.032998E-01 1313 194 8.657580E-02 2.084603E-01 2.020010E-01 1313 195 8.699560E-02 2.479205E-01 2.003244E-01 1313 196 8.741540E-02 4.011803E-01 2.024286E-01 1313 197 8.783530E-02 2.268201E-01 2.019844E-01 1313 198 8.825510E-02 3.026796E-01 2.041462E-01 1313 199 8.867490E-02 2.258202E-01 2.168412E-01 1313 200 8.909480E-02 2.951394E-01 2.027552E-01 1313 201 8.951460E-02 2.959998E-01 2.040658E-01 1313 202 8.993440E-02 1.981796E-01 2.053174E-01 1313 203 9.035420E-02 2.770604E-01 2.057294E-01 1313 204 9.077410E-02 4.247396E-01 2.082850E-01 1313 205 9.119390E-02 2.309202E-01 2.045500E-01 1313 206 9.161370E-02 8.179994E-02 2.021568E-01 1313 207 9.203360E-02 8.209994E-02 2.077024E-01 1313 208 9.245340E-02 2.830806E-01 2.059344E-01 1313 209 9.287320E-02 8.016048E-02 2.064270E-01 1313 210 9.329310E-02 1.602597E-01 2.098022E-01 1313 211 9.371290E-02 1.151197E-01 2.101692E-01 1313 212 9.413270E-02 7.199948E-02 2.066174E-01 1313 213 9.455250E-02 3.361970E-02 2.050366E-01 1313 214 9.497240E-02 5.500002E-02 2.079150E-01 1313 215 9.539220E-02 1.519203E-01 2.047176E-01 1313 216 9.581200E-02 2.450601E-01 2.020096E-01 1313 217 9.623190E-02 3.270398E-01 2.052066E-01 1313 218 9.665170E-02 2.035601E-01 2.008346E-01 1313 219 9.707150E-02 2.635998E-01 2.054526E-01 1313 220 9.749140E-02 1.291399E-01 2.004134E-01 1313 221 9.791120E-02 2.406400E-01 2.000132E-01 1313 222 9.833100E-02 2.477799E-01 1.997344E-01 1313 223 9.875080E-02 8.526040E-02 1.926772E-01 1313 224 9.917070E-02 -7.853920E-02 1.975730E-01 1313 225 9.959050E-02 1.703800E-01 1.984304E-01 1313 226 1.000100E-01 2.004797E-01 1.978270E-01 1313 227 1.004300E-01 1.876402E-01 1.973316E-01 1313 228 1.008500E-01 1.283600E-01 1.915172E-01 1313 229 1.012700E-01 7.316036E-02 1.911020E-01 1313 230 1.016900E-01 1.975199E-01 1.903784E-01 1313 231 1.021090E-01 2.649603E-01 1.924862E-01 1313 232 1.025290E-01 1.940195E-01 1.918786E-01 1313 233 1.029490E-01 2.177598E-01 1.936238E-01 1313 234 1.033690E-01 1.998400E-01 1.942324E-01 1313 235 1.037890E-01 1.303196E-01 1.895796E-01 1313 236 1.042090E-01 2.704996E-01 1.936556E-01 1313 237 1.046280E-01 8.920000E-02 1.874124E-01 1313 238 1.050480E-01 1.408002E-01 1.903782E-01 1313 239 1.054680E-01 1.536199E-01 1.954538E-01 1313 240 1.058880E-01 1.099402E-01 1.882188E-01 1313 241 1.063080E-01 7.070054E-02 1.887894E-01 1313 242 1.067280E-01 7.852050E-02 1.860012E-01 1313 243 1.071470E-01 2.071604E-01 1.902154E-01 1313 244 1.075670E-01 3.370803E-01 1.923162E-01 1313 245 1.079870E-01 6.001976E-02 1.897920E-01 1313 246 1.084070E-01 1.699005E-01 1.924014E-01 1313 247 1.088270E-01 2.927948E-02 1.855280E-01 1313 248 1.092470E-01 1.422200E-01 1.866040E-01 1313 249 1.096660E-01 6.981960E-02 1.868324E-01 1313 250 1.100860E-01 6.187968E-02 1.847388E-01 1313 251 1.105060E-01 5.813980E-02 1.846958E-01 1313 252 1.109260E-01 1.756400E-01 1.845364E-01 1313 253 1.113460E-01 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