{ "code": "SASDB25", "status": "Published", "type_of_curve": "Extrapolated to infinite dilution", "angular_unit": "1/nm", "project": { "title": "Clinically Linked Mutations in the Central Domains of Cardiac Myosin-Binding Protein C with Distinct Phenotypes Show Differential Structural Effects.", "publication": { "title": "Clinically Linked Mutations in the Central Domains of Cardiac Myosin-Binding Protein C with Distinct Phenotypes Show Differential Structural Effects.", "author_list": "Nadvi NA, Michie KA, Kwan AH, Guss JM, Trewhella J", "journal": "Structure", "doi": "10.1016/j.str.2015.11.001", "pmid": "26688216", "published_date": "2016 Jan 5" }, "status": "released", "submitted_date": "2016-06-25", "released_date": "2017-12-12" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDB25.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDB25.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB25_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB25_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB25_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB25_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB25.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "Australian Synchrotron", "city": "Melbourne", "country": "Australia", "beamline_name": "SAXS/WAXS", "beam_geometry": "Point", "type_of_source": "X-ray synchrotron", "point_source": true, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Cardiac myosin binding protein-C: domains C5-C6-C7", "short_name": "cMyBP-C", "sequence": "GPGSRQEPPKIHLDCPGRIPDTIVVVAGNKLRLDVPISGDPAPTVIWQKAITQGNKAPARPAPDAPEDTGDSDEWVFDKKLLCETEGRVRVETTKDRSIFTVEGAEKEDEGVYTVTVKNPVGEDQVNLTVKVIDVPDAPAAPKISNVGEDSCTVQWEPPAYDGGQPILGYILERKKKKSYRWMRLNFDLIQELSHEARRMIEGVVYEMRVYAVNAIGMSRPSPASQPFMPIGPPSEPTHLAVEDVSDTTVSLKWRPPERVGAGGLDGYSVEYCPEGCSEWVAALQGLTEHTSILVKDLPTGARLLFRVRAHNMAGPGAPVTTTEPVTV", "organism": "Homo sapiens", "uniprot_code": "Q14896", "uniprot_range_first": 641, "uniprot_range_last": 964, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MPEPGKKPVSAFSKKPRSVEVAAGSPAVFEAETERAGVKVRWQRGGSDISASNKYGLATE\nGTRHTLTVREVGPADQGSYAVIAGSSKVKFDLKVIEAEKAEPMLAPAPAPAEATGAPGEA\nPAPAAELGESAPSPKGSSSAALNGPTPGAPDDPIGLFVMRPQDGEVTVGGSITFSARVAG\nASLLKPPVVKWFKGKWVDLSSKVGQHLQLHDSYDRASKVYLFELHITDAQPAFTGSYRCE\nVSTKDKFDCSNFNLTVHEAMGTGDLDLLSAFRRTSLAGGGRRISDSHEDTGILDFSSLLK\nKRDSFRTPRDSKLEAPAEEDVWEILRQAPPSEYERIAFQYGVTDLRGMLKRLKGMRRDEK\nKSTAFQKKLEPAYQVSKGHKIRLTVELADHDAEVKWLKNGQEIQMSGSKYIFESIGAKRT\nLTISQCSLADDAAYQCVVGGEKCSTELFVKEPPVLITRPLEDQLVMVGQRVEFECEVSEE\nGAQVKWLKDGVELTREETFKYRFKKDGQRHHLIINEAMLEDAGHYALCTSGGQALAELIV\nQEKKLEVYQSIADLMVGAKDQAVFKCEVSDENVRGVWLKNGKELVPDSRIKVSHIGRVHK\nLTIDDVTPADEADYSFVPEGFACNLSAKLHFMEVKIDFVPRQEPPKIHLDCPGRIPDTIV\nVVAGNKLRLDVPISGDPAPTVIWQKAITQGNKAPARPAPDAPEDTGDSDEWVFDKKLLCE\nTEGRVRVETTKDRSIFTVEGAEKEDEGVYTVTVKNPVGEDQVNLTVKVIDVPDAPAAPKI\nSNVGEDSCTVQWEPPAYDGGQPILGYILERKKKKSYRWMRLNFDLIQELSHEARRMIEGV\nVYEMRVYAVNAIGMSRPSPASQPFMPIGPPSEPTHLAVEDVSDTTVSLKWRPPERVGAGG\nLDGYSVEYCPEGCSEWVAALQGLTEHTSILVKDLPTGARLLFRVRAHNMAGPGAPVTTTE\nPVTVQEILQRPRLQLPRHLRQTIQKKVGEPVNLLIPFQGKPRPQVTWTKEGQPLAGEEVS\nIRNSPTDTILFIRAARRVHSGTYQVTVRIENMEDKATLVLQVVDKPSPPQDLRVTDAWGL\nNVALEWKPPQDVGNTELWGYTVQKADKKTMEWFTVLEHYRRTHCVVPELIIGNGYYFRVF\nSQNMVGFSDRAATTKEPVFIPRPGITYEPPNYKALDFSEAPSFTQPLVNRSVIAGYTAML\nCCAVRGSPKPKISWFKNGLDLGEDARFRMFSKQGVLTLEIRKPCPFDGGIYVCRATNLQG\nEARCECRLEVRVPQ", "mw": 35.751, "total_mw": 35.751, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM Tris-HCl, 250 mM NaCl, 2 mM TCEP, 0.02% sodium azide", "concentration_unit": "mM", "comment": null, "additive": "250 mM NaCl, 2 mM TCEP, 0.02% sodium azide", "concentration": 25.0, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Cardiac myosin binding protein-C: domains C5-C6-C7", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Sydney, New South Wales 2006, Australia", "full_name": "School of Molecular Bioscience, University of Sydney.", "webpage": "" } ], "contributor_name": "Jill", "contributor_surname": "Trewhella", "orcid": null } ], "concentration_method": null, "concentration_unit": "mg/ml", "date": "2015-04-18", "storage_temperature": 22.0, "cell_temperature": 22.0, "exposure_time": 1.0, "number_of_frames": 44, "wavelength": null, "sample_detector_distance": 1.6, "concentration_min": 0.7, "concentration_max": 3.4, "sample_volume": null, "flow_rate": null, "s_min": 0.057, "s_max": 3.436, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "X-ray synchrotron radiation scattering data from solutions of human cardiac myosin binding protein-C, domains C5-C6-C7 in 25 mM TrisHCl 250 mM NaCl, 2 mM TCEP, 0.02% sodium azide were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M-W pixel detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.10332 nm). Different solute concentrations in the range 0.70-3.40 mg/ml were measured using a total exposure time of 44 s (recorded as 44 x 1 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data presented here represent the SAXS profile extrapolated to zero concentration calculated from the solute concentration series.", "tags": [], "intensity_unit": null, "experimental_mw": 38.2, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 0.1068, "pddf_i0_error": null, "guinier_i0": 0.1055, "guinier_i0_error": 0.0001, "pddf_rg": 3.931, "pddf_rg_error": 0.001, "guinier_rg": 3.75, "guinier_rg_error": 0.01, "pddf_dmax": 14.1, "pddf_dmax_error": null, "porod_volume": 54.7, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 15, "guinier_point_last": 75, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDB25_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }