{ "code": "SASDB27", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA.", "publication": { "title": "Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA.", "author_list": "Arentson BW, Hayes EL, Zhu W, Singh H, Tanner JJ, Becker DF", "journal": "Biosci Rep", "doi": "10.1042/BSR20160435", "pmid": "27742866", "published_date": "2016 Dec" }, "status": "released", "submitted_date": "2016-10-10", "released_date": "2016-10-16" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDB27.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDB27.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB27_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB27_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB27.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "MAR 165 CCD", "resolution": null }, "name": "Advanced Light Source (ALS)", "city": "Berkeley, CA", "country": "USA", "beamline_name": "12.3.1 (SIBYLS)", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Proline utilization A", "short_name": "PutA", "sequence": "MGSSHHHHHHSSGLVPRGSH\r\nMGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSY\r\nLEQLENSDTLPEH\r\nMTDLSALGPKAKFAPEAEVLQALVAQAALPQPQLDRIAARGADLVAR\r\nIRAEAKPSLMEHFLAQYGLSTREGVALMCLAEAMLRVPDTATIDALIEDKIAPSDWGKHL\r\nGTAASSLVNASTWALMLTGKVLDDGAGGIAGTLRGAMRRLGEPVIRAAVGQAMREMGRQF\r\nVLGETIEKALERAEKREAEGYTFSYDMLGEAALTAADAERYRLAYAQAITAIGKAATRGS\r\nIAANPGISIKLSALHPRYEVAQEARVMAELVPVVRDLARAAARAGIALHIDAEEQDRLAL\r\nSLRVMAAVIADPETAGWEGFGAVVQAYGKRAGAAIDALAAMARAAGRRINIRLVKGAYWD\r\nAEMKRAQVEGHPGFPLFTSKTGTDVAYICLAAKLFGLNDCIYPQFATHNAHTVAAVLEMA\r\nAGRPFEFQRLHGMGARLHDIVLRETGGRCRIYAPVGAHRDLLAYLVRRLLENGANSSFVN\r\nQIVNESVPPAEVAACPFAALPTARAPRGLLAPADLFGAGRVNAQGFDLSDPEVLARIEAA\r\nRDVTLPDAAPIVAGPVSGTLRPVRNPATGAVVAQVTEADAATVALALDAAQVWSAPAATR\r\nAAVLCRAADLYEENFGPIFAALAQEAGKTLGDAVSELREAVDFLRYYAAEGAADTRPPRG\r\nAVVAISPWNFPLAIFTGQVAAALMAGNAVLAKPAEQTPIIAALAVRLLHQAGVPETALQL\r\nLPGDGPTVGAALTRDPRVAGVVFTGSTETAQIIARAMAAHLAPGTPLIAETGGLNAMVVD\r\nSTALPEQAVRDVVASAFRSAGQRCSALRCLYVQDDIAPHLIGMLKGAMEELVSGDPARLS\r\nTDVGPVIDAEAKAGIETYLAANKARILHRSTAPEGGHFVAPALLQVGGIADLEREIFGPV\r\nLHLATFAAEDLPAVIAAINARGYGLTFGLHSRIDARVETVAETIRAGNIYVNRNQIGAVV\r\nGSQPFGGEGLSGTGPKAGGPLYLNRFYAPEPVVAVGGWTEAATPILPEARETQLDEIFLP\r\nGPTGELNRLTRHQRGPILCLGPGAEAASAQAAAVVALGGQAVQASGAVSPKALETLTPLA\r\nGVLWWGAAEMGRAYAQALAVRPGPLVPLITAKPDLAHVAHERHLCVDTTAAGGNAALLAG", "organism": "Escherchia coli, Rhodobacter capsulatus", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 125.528, "total_mw": 251.056, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "50 mM Tris, 200 mM NaCl, 0.5 mM Tris(3-hydroxypropyl)phosphine", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Chimeric EcRHH-RcPutA: The E.coli Proline utilization A RHH domain fused to R.capsulatus PutA", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "Mizzou", "address": "Columbia, MO, USA", "full_name": "University of Missouri-Columbia", "webpage": "http://missouri.edu/" } ], "contributor_name": "John", "contributor_surname": "Tanner", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2012-10-16", "storage_temperature": 4.0, "cell_temperature": 20.0, "exposure_time": 0.5, "number_of_frames": 2, "wavelength": 0.1, "sample_detector_distance": 1.5, "concentration_min": null, "concentration_max": 6.0, "sample_volume": null, "flow_rate": null, "s_min": 0.112, "s_max": 3.165, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "GNOM", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of a chimeric EcRHH-RcPutA protein fusion in 50 mM Tris, 200 mM NaCl, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH 7.5 were collected on the 12.3.1 (SIBYLS) camera on the storage ring Advanced Light Source (ALS) (Berkeley, CA, USA) using a MAR 165 CCD detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). A solute concentrations of 6 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The merged SAXS curve displayed in this entry was obtained by merging the low-s region (points 2-140, s=0.1125 — 0.9517 nm-1) from an image collected with an exposure time of 0.5 s with the higher-s region (points 19-505, s=0.2159 — 3.1710 nm-1) of a profile collected with exposure time of 2.0 s. The errors in PDDF Rg and I0 were estimated by running GNOM via primus/qt (r4556) assuming Dmax in the range of 173-193 and using all points of the scattering curve (1-503).", "tags": [], "intensity_unit": null, "experimental_mw": 251.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 3033.0, "pddf_i0_error": 5.5, "guinier_i0": 3011.44, "guinier_i0_error": 11.0, "pddf_rg": 5.41, "pddf_rg_error": 0.008, "guinier_rg": 5.2, "guinier_rg_error": 0.02, "pddf_dmax": 18.3, "pddf_dmax_error": 1.0, "porod_volume": 308.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 23, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDB27_datloglog_img.png", "symmetry": null, "last_modified": "2026-05-18T10:07:55.648439+02:00", "bragg_peak": [] }