{
  "code": "SASDW27",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "The extracellular domain of SaNSrFP binds bacitracin and allows the identification of new members of the BceAB transporter family",
    "publication": {
      "title": "The extracellular domain of SaNSrFP binds bacitracin and allows the identification of new members of the BceAB transporter family",
      "author_list": "Mammen C, Gottstein J, Cea P, Tantsur K, Reiners J, Bonus M, Gohlke H, Smits S",
      "journal": "Frontiers in Microbiology",
      "doi": "10.3389/fmicb.2025.1662803",
      "pmid": null,
      "published_date": "2025 Sep 17"
    },
    "status": "released",
    "submitted_date": "2025-02-04",
    "released_date": "2025-09-01"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDW27.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDW27.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW27_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW27_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDW27_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDW27_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDW27.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "ABC transporter ATP-binding protein",
          "short_name": "SaNsrF",
          "sequence": "MGHHHHHHHHHHSSGHIEGRHMLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESGSGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLSVRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLITNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKDGRIFHQLYRGNKNNSEFNKDISLTMSAILGGE",
          "organism": "Streptococcus agalactiae",
          "uniprot_code": "X5KGL2",
          "uniprot_range_first": 1,
          "uniprot_range_last": 250,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESGSGKTTLLNILATLEKP\nTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLSVRDNIYLPLVLDRKRYKE\nMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLITNPQILLADEPTAALDYRNS\nEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKDGRIFHQLYRGNKNNSEFNKDIS\nLTMSAILGGE",
          "mw": 30.895,
          "total_mw": 61.79,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "ATP-binding protein, part from the whole SaNsrFP complex\r\nAmino acid 1-21 belongs to an artificial His-tag and a thrombin cleavage site."
        },
        {
          "long_name": "ABC transporter, permease protein",
          "short_name": "SaNsrP",
          "sequence": "MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVGRDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVASLELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIFLTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAPALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMKQNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFEEKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDFRRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGLIIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVGNISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQEVGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSGLTVLAISIIYFIIYRITSRTYYHIIER",
          "organism": "Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)",
          "uniprot_code": "Q8DZX0",
          "uniprot_range_first": 1,
          "uniprot_range_last": 651,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVGRDMGTAATVLFLGVIV\nLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVASLELFIIYIFLISIGSLFS\nAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIFLTLEVPVIRHVHLSSPLSLF\nRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAPALAVIYRFFFAVLLVIAGTYLF\nYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMKQNAVGLASITLLAVMALVTIATTV\nSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFEEKILKKLGKSSKEAITYNQTMISMPV\nSQSSELNITSKNVKHVDITKTGFMYLITQNDFRRLGHQLPKLKDNQVAYFVQKGDSRLKK\nINLLGNKFDVVKNLKEAYVPETTNTYNPGLIIFANNKQIDNIRKAYLPYTKNINTFPKTF\nKAYLDLNSQEINSISKNDIIEVDGKYVGNISTKQSFLKEGYQMFGGLLFTGFLLGISFLL\nGIALIVYYKQYSEGHEDKRSYRILQEVGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVA\nIPMLKQMLLVFGVLNSTIVYVVSGLTVLAISIIYFIIYRITSRTYYHIIER",
          "mw": 74.016,
          "total_mw": 74.016,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
        "name": "50 mM Tris, 300 mM NaCl, 10 % Glycerol, 0.005 % LMNG,",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "ABC transporter ATP-binding protein - ABC transporter, permease protein - complex from S.agalactiae (SaNsrFP)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Düsseldorf, Germany",
            "full_name": "Heinrich Heine University Düsseldorf",
            "webpage": "https://www.uni-duesseldorf.de"
          }
        ],
        "contributor_name": "Jens",
        "contributor_surname": "Reiners",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-04-25",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 1.0,
    "number_of_frames": 10,
    "wavelength": 0.099,
    "sample_detector_distance": 2.813,
    "concentration_min": null,
    "concentration_max": 0.5,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.083,
    "s_max": 3.664,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "1/cm",
  "experimental_mw": 148.3,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 401.0,
  "porod_mw_error": null,
  "pddf_i0": 148.3,
  "pddf_i0_error": 0.54,
  "guinier_i0": 148.299,
  "guinier_i0_error": null,
  "pddf_rg": 6.004,
  "pddf_rg_error": 0.03,
  "guinier_rg": 5.83,
  "guinier_rg_error": 0.03,
  "pddf_dmax": 20.5,
  "pddf_dmax_error": null,
  "porod_volume": 734.55,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 28,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDW27_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-09-17T10:18:55.539245+02:00",
  "bragg_peak": []
}