{ "code": "SASDA28", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.", "publication": { "title": "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.", "author_list": "Chen X, Hnida K, Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM", "journal": "J Biol Chem", "doi": "10.1074/jbc.M115.669895", "pmid": "26160175", "published_date": "2015 Aug 28" }, "status": "released", "submitted_date": "2015-04-14", "released_date": "2015-08-28" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDA28.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDA28.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA28_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA28_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDA28_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA28_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDA28.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "anti-TG2 antibody (679 14 E06) ", "short_name": null, "sequence": "EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIGWVRQMPGKGLEWMGIIYPGDSDTRY SPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARPHYYDSLDAFDIWGQGTMVTVSS ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS GLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQRPGKAPKLLIYKASSLESGVPS RFSGSGSGTEFTLTISSLQPDDFATYYCQHYNSYSPGYTFGQGTKVEIK RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDS KDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC\r\n", "organism": null, "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 47.639, "total_mw": 47.639, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris 150mM NaCl 1mM EDTA", "concentration_unit": "mM", "comment": null, "additive": "150mM NaCl, 1mM EDTA", "concentration": 20.0, "pka": null, "ph": 7.2, "deuteration": null }, "purity_method": null, "name": "anti-TG2 antibody (679 14 E06)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Melissa ", "contributor_surname": "Graewert", "orcid": null } ], "concentration_method": null, "concentration_unit": "mg/ml", "date": "2015-01-17", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 0.05, "number_of_frames": 20, "wavelength": 0.12, "sample_detector_distance": 3.1, "concentration_min": 2.0, "concentration_max": 16.0, "sample_volume": null, "flow_rate": null, "s_min": 0.202, "s_max": 3.969, "total_exposure_time": 1.0, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA28_fit1_model1_img.png", "software": "CRYSOL", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA28_fit1_model1.pdb", "model_mw": 47.68, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 14 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA28_fit1_fit_img.png", "software": null, "chi_square_value": 1.13, "p_value": 0.0, "fit_residual_plot": "fit4columns269_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA28_fit1.fit", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": "Structural basis for antigen recognition by transglutaminase 2-specific autoantibodies in celiac disease --- SAXS profile of the autoantibody", "experiment_description": null, "tags": [], "intensity_unit": null, "experimental_mw": 47.6, "experimental_mw_error": null, "guinier_i0_mw": 47.6, "guinier_i0_mw_error": null, "porod_mw": 47.5, "porod_mw_error": null, "pddf_i0": 233.1, "pddf_i0_error": null, "guinier_i0": 1158.9, "guinier_i0_error": 6.2, "pddf_rg": 2.58, "pddf_rg_error": null, "guinier_rg": 2.54, "guinier_rg_error": 0.02, "pddf_dmax": 8.1, "pddf_dmax_error": 0.5, "porod_volume": 58.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 119, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDA28_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }