{ "code": "SASDB28", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Structure and activation of C1, the complex initiating the classical pathway of the complement cascade.", "publication": { "title": "Structure and activation of C1, the complex initiating the classical pathway of the complement cascade.", "author_list": "Mortensen SA, Sander B, Jensen RK, Pedersen JS, Golas MM, Jensenius JC, Hansen AG, Thiel S, Andersen GR", "journal": "Proc Natl Acad Sci U S A", "doi": "10.1073/pnas.1616998114", "pmid": "28104818", "published_date": "2017 Jan 31" }, "status": "released", "submitted_date": "2016-12-02", "released_date": "2017-01-09" }, "pddf_data": null, "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDB28.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB28_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB28_kratky_img.png", "pddf_plot": null, "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB28_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB28.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Complement C1q subcomponent subunit C", "short_name": "C1qc", "sequence": "EDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIRTGIQGLKGDQGEPGPSGNPGKV\r\nGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAIRRNPPMGGNVVIFDTVITNQEE\r\nPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSRGQVRRSLGFCDTTNKGLFQVVS\r\nGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFLIFPSA", "organism": "Homo sapiens", "uniprot_code": "P02747", "uniprot_range_first": 23, "uniprot_range_last": 245, "oligomerization": "hexamer", "molecular_type": "protein", "uniprot_sequence": "MDVGPSSLPHLGLKLLLLLLLLPLRGQANTGCYGIPGMPGLPGAPGKDGYDGLPGPKGEP\nGIPAIPGIRGPKGQKGEPGLPGHPGKNGPMGPPGMPGVPGPMGIPGEPGEEGRYKQKFQS\nVFTVTRQTHQPPAPNSLIRFNAVLTNPQGDYDTSTGKFTCKVPGLYYFVYHASHTANLCV\nLLYRSGVKVVTFCGHTSKTNQVNSGGVLLRLQVGEEVWLAVNDYYDMVGIQGSDSVFSGF\nLLFPD", "mw": 23.687, "total_mw": 142.122, "number_molecules": 6, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Complement C1q subcomponent subunit B", "short_name": "C1qb", "sequence": "QLSCTGPPAIPGIPGIPGTPGPDGQPGTPGIKGEKGLPGLAGDHGEFGEKGDPGIPGNPG\r\nKVGPKGPMGPKGGPGAPGAPGPKGESGDYKATQKIAFSATRTINVPLRRDQTIRFDHVIT\r\nNMNNNYEPRSGKFTCKVPGLYYFTYHASSRGNLCVNLMRGRERAQKVVTFCDYAYNTFQV\r\nTTGGMVLKLEQGENVFLQATDKNSLLGMEGANSIFSGFLLFPDMEA", "organism": "Homo sapiens", "uniprot_code": "P02746", "uniprot_range_first": 28, "uniprot_range_last": 253, "oligomerization": "hexamer", "molecular_type": "protein", "uniprot_sequence": "MMMKIPWGSIPVLMLLLLLGLIDISQAQLSCTGPPAIPGIPGIPGTPGPDGQPGTPGIKG\nEKGLPGLAGDHGEFGEKGDPGIPGNPGKVGPKGPMGPKGGPGAPGAPGPKGESGDYKATQ\nKIAFSATRTINVPLRRDQTIRFDHVITNMNNNYEPRSGKFTCKVPGLYYFTYHASSRGNL\nCVNLMRGRERAQKVVTFCDYAYNTFQVTTGGMVLKLEQGENVFLQATDKNSLLGMEGANS\nIFSGFLLFPDMEA", "mw": 23.742, "total_mw": 142.452, "number_molecules": 6, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Complement C1q subcomponent subunit A", "short_name": "C1qa", "sequence": "EDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIRTGIQGLKGDQGEPGPSGNPGKV\r\nGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAIRRNPPMGGNVVIFDTVITNQEE\r\nPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSRGQVRRSLGFCDTTNKGLFQVVS\r\nGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFLIFPSA", "organism": "Homo sapiens", "uniprot_code": "P02745", "uniprot_range_first": 23, "uniprot_range_last": 245, "oligomerization": "hexamer", "molecular_type": "protein", "uniprot_sequence": "MEGPRGWLVLCVLAISLASMVTEDLCRAPDGKKGEAGRPGRRGRPGLKGEQGEPGAPGIR\nTGIQGLKGDQGEPGPSGNPGKVGYPGPSGPLGARGIPGIKGTKGSPGNIKDQPRPAFSAI\nRRNPPMGGNVVIFDTVITNQEEPYQNHSGRFVCTVPGYYYFTFQVLSQWEICLSIVSSSR\nGQVRRSLGFCDTTNKGLFQVVSGGMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFSGFL\nIFPSA", "mw": 23.687, "total_mw": 142.122, "number_molecules": 6, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "50 mM TrisHCl, 145 mM NaCl, 3 mM CaCl2", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "Complement factor 1q (C1q)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Aarhus, Denmark", "full_name": "Aarhus University", "webpage": "http://www.au.dk" } ], "contributor_name": "Rasmus", "contributor_surname": "Jensen", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2014-12-08", "storage_temperature": 4.0, "cell_temperature": 4.0, "exposure_time": 2.0, "number_of_frames": 10, "wavelength": 0.0992, "sample_detector_distance": null, "concentration_min": null, "concentration_max": 3.66, "sample_volume": null, "flow_rate": null, "s_min": 0.036, "s_max": 4.975, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": null, "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Complement factor 1q (C1q) in 50 mM TrisHCl, 145 mM NaCl, 3 mM CaCl2, pH 7.4, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 3.7 and 3.7 mg/ml were measured at 4°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.", "tags": [], "intensity_unit": null, "experimental_mw": 407.0, "experimental_mw_error": null, "guinier_i0_mw": 407.0, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 534.52, "guinier_i0_error": 0.97, "pddf_rg": null, "pddf_rg_error": null, "guinier_rg": 12.61, "guinier_rg_error": 0.17, "pddf_dmax": null, "pddf_dmax_error": null, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 4, "guinier_point_last": 15, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDB28_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }