{ "code": "SASDA35", "status": "Published", "type_of_curve": "Extrapolated to infinite dilution", "angular_unit": "1/nm", "project": { "title": "The Salmonella enterica ZinT structure, zinc affinity and interaction with the high-affinity uptake protein ZnuA provide insight into the management of periplasmic zinc.", "publication": { "title": "The Salmonella enterica ZinT structure, zinc affinity and interaction with the high-affinity uptake protein ZnuA provide insight into the management of periplasmic zinc.", "author_list": "Ilari A, Alaleona F, Tria G, Petrarca P, Battistoni A, Zamparelli C, Verzili D, Falconi M, Chiancone E", "journal": "Biochim Biophys Acta", "doi": "10.1016/j.bbagen.2013.10.010", "pmid": "24128931", "published_date": "2014 Jan" }, "status": "released", "submitted_date": null, "released_date": "2014-07-01" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDA35.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDA35.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA35_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA35_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDA35_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA35_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDA35.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "High-affinity zinc transporter periplasmic component", "short_name": "ZinT", "sequence": ">tr|V2JZU2|V2JZU2_SALET High-affinity zinc transporter periplasmic component (Fragment) OS=Salmonella enterica subsp. enterica serovar Nchanga str. CFSAN001092 GN=znuA PE=4 SV=1\r\nFAALSAALWGSATQAADAAVVASLKPLGFIASAIADGVTDTQVLLPDGASEHDYSLRPSD\r\nVKRLQGADLVVWVGPEMEAFMEKSVRNIPDNKQVTIAQLADVKPLLMKGADDDEDEHAHT\r\nGADEEKGDVHHHHGEYNMHLWLSPEIARATAVAIHEKLVELMPQSRAKLDANLKDFEAQL\r\nAATDKQVGNELAPLKGKGYFVFHDAYGYYEKHYGLTPLGHFTVNPEIQPGAQRLHEIRTQ\r\nLVEQKATCVFAEPQFRPAVVEAVARGTSVRMGTLDPLGTNIKLGKTSYSAFLSQLANQYA\r\nSCLKGD", "organism": "Salmonella enterica subsp. enterica serovar Nchanga", "uniprot_code": "V2JZU2", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 33.25, "total_mw": 33.25, "number_molecules": 1, "complex_state": null, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Zinc/cadmium-binding protein", "short_name": "SeZnuA", "sequence": ">tr|L6R5I5|L6R5I5_SALEN Zinc/cadmium-binding protein OS=Salmonella enterica subsp. enterica serovar Enteritidis str. 543463 40-18 GN=SEEE4018_10142 PE=4 SV=1\r\nMLLVNSPAFAHGHHAHGAPMTEVEQKAAAGVFDDANVRDRALTDWDGMWQSVYPYLVSGE\r\nLDPVFRQKAKKDPEKTFEDIKAYYRKGYVTNVETIGIENGVIEFHRDNNVASCKYNYAGY\r\nKILTYASGKKGVRYLFECKDANSKAPKYVQFSDHIIAPRKSAHFHIFMGNTSQQALLQEM\r\nENWPTYYPYQLKANEVVDEMLHH", "organism": "Salmonella enterica subsp. enterica serovar Enteritidis", "uniprot_code": "L6R5I5", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 23.24, "total_mw": 23.24, "number_molecules": 1, "complex_state": null, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "50 mM HEPES 50 mM KCl", "concentration_unit": "mM", "comment": "4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid", "additive": "KCl 50.000 mM", "concentration": 50.0, "pka": 7.0, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "SeZinT-SeZnuA complex", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Giancarlo", "contributor_surname": "Tria", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2013-05-09", "storage_temperature": 4.0, "cell_temperature": 4.0, "exposure_time": 1.0, "number_of_frames": 10, "wavelength": 0.93, "sample_detector_distance": 2.43, "concentration_min": 0.17, "concentration_max": 5.0, "sample_volume": null, "flow_rate": null, "s_min": 0.05, "s_max": 3.6, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA35_fit1_model1_img.png", "software": "BUNCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA35_fit1_model1.pdb", "model_mw": 53.58, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 13 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA35_fit1_fit_img.png", "software": null, "chi_square_value": 1.317904, "p_value": 0.0, "fit_residual_plot": "SASDA35_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA35_fit1.fit", "fit_log": null, "software_version": null, "description": null }, { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA35_fit2_model1_img.png", "software": "DAMMIF", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA35_fit2_model1.pdb", "model_mw": null, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 13 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA35_fit2_fit_img.png", "software": null, "chi_square_value": null, "p_value": 0.0, "fit_residual_plot": null, "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA35_fit2.fit", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": "BSA", "description": null, "experiment_description": null, "tags": [], "intensity_unit": null, "experimental_mw": 43.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 45.55, "pddf_i0_error": null, "guinier_i0": 45.524, "guinier_i0_error": null, "pddf_rg": 2.5, "pddf_rg_error": null, "guinier_rg": 2.5, "guinier_rg_error": null, "pddf_dmax": 8.5, "pddf_dmax_error": null, "porod_volume": 55.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 45, "guinier_point_last": 105, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": "https://www.sasbdb.org/media/standard_curves/BSa_002.dat", "intensities_log_log_plot": "SASDA35_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }