{ "code": "SASDA48", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.", "publication": { "title": "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease.", "author_list": "Chen X, Hnida K, Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM", "journal": "J Biol Chem", "doi": "10.1074/jbc.M115.669895", "pmid": "26160175", "published_date": "2015 Aug 28" }, "status": "released", "submitted_date": "2015-04-14", "released_date": "2015-08-28" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDA48.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDA48.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA48_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA48_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDA48_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA48_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDA48.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "anti-TG2 antibody (679 14 E06) ", "short_name": null, "sequence": "EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIGWVRQMPGKGLEWMGIIYPGDSDTRY SPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARPHYYDSLDAFDIWGQGTMVTVSS ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS GLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQRPGKAPKLLIYKASSLESGVPS RFSGSGSGTEFTLTISSLQPDDFATYYCQHYNSYSPGYTFGQGTKVEIK RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDS KDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC\r\n", "organism": null, "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 47.639, "total_mw": 47.639, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "transglutaminase 2", "short_name": "TGA", "sequence": "MGSSHHHHHHSSGLVPRGSH maeelvlerc dleletngrd hhtadlcrek lvvrrgqpfw ltlhfegrny easvdsltfs vvtgpapsqe agtkarfplr daveegdwta tvvdqqdctl slqlttpana piglyrlsle astgyqgssf vlghfillfn awcpadavyl dseeerqeyv ltqqgfiyqg sakfiknipw nfgqfedgil diclilldvn pkflknagrd csrrsspvyv grvvsgmvnc nddqgvllgr wdnnygdgvs pmswigsvdi lrrwknhgcq rvkygqcwvf aavactvlrc lgiptrvvtn ynsahdqnsn llieyfrnef geiqgdksem iwnfhcwves wmtrpdlqpg yegwqaldpt pqeksegtyc cgpvpvraik egdlstkyda pfvfaevnad vvdwiqqddg svhksinrsl ivglkistks vgrderedit htykypegss eereaftran hlnkl aekee tgmamrirvg qsmnmgsdfd vfahitnnta eeyvcrlllc artvsyngil gpecgtkyll nlnlepfsek svplcilyek yrdcltesnl ikvrallvep vinsyllaer dlylenpeik irilgepkqk rklvaevslq nplpvalegc tftvegaglt eeqktveipd pveageevkv rmdllplhmg lhklvvnfes dklkavkgfr nviigpa", "organism": "Homo sapiens", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 79.491, "total_mw": 79.491, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris 150mM NaCl 1mM EDTA", "concentration_unit": "mM", "comment": null, "additive": "150mM NaCl, 1mM EDTA", "concentration": 20.0, "pka": null, "ph": 7.2, "deuteration": null }, "purity_method": null, "name": "transglutaminase2:anti-transglutaminase2 FAB1 antibody complex", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Melissa ", "contributor_surname": "Graewert", "orcid": null } ], "concentration_method": null, "concentration_unit": "mg/ml", "date": "2015-01-17", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 0.05, "number_of_frames": 20, "wavelength": 0.12, "sample_detector_distance": 3.1, "concentration_min": null, "concentration_max": 9.0, "sample_volume": null, "flow_rate": null, "s_min": 0.144, "s_max": 2.507, "total_exposure_time": 1.0, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA48_fit1_model1_img.png", "software": "DAMMIN", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA48_fit1_model1.pdb", "model_mw": 106.3, "bead_radius": 3.5, "log": null, "symmetry": "", "comment": "", "user": 14 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA48_fit1_fit_img.png", "software": null, "chi_square_value": 0.72, "p_value": 0.157, "fit_residual_plot": "SASDA48_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA48_fit1.fir", "fit_log": null, "software_version": null, "description": null }, { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA48_fit2_model1_img.png", "software": "SASREF", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA48_fit2_model1.pdb", "model_mw": 124.4, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 14 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA48_fit2_fit_img.png", "software": null, "chi_square_value": 0.66, "p_value": 0.053, "fit_residual_plot": "SASDA48_fit2_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA48_fit2.fit", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": "Structural basis for antigen recognition by transglutaminase 2-specific autoantibodies in celiac disease. 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