{ "code": "SASDD52", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP.", "publication": { "title": "Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP.", "author_list": "Slonimskiy YB, Maksimov EG, Lukashev EP, Moldenhauer M, Jeffries CM, Svergun DI, Friedrich T, Sluchanko NN", "journal": "Biochim Biophys Acta", "doi": "10.1016/j.bbabio.2018.03.001", "pmid": "29524381", "published_date": "2018 May" }, "status": "released", "submitted_date": null, "released_date": "2018-03-10" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDD52.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDD52.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD52_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD52_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD52_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD52_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDD52.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Uncharacterized fluorescence recovery protein", "short_name": "FRP", "sequence": "MSEINWSEQDKNAAVDALHKAYEREVKILIDEVKQKAFNLTEIDEVWKLHDFLSARRHDI\r\nDGKYDYRDAASVFVLATLVKQGWLSLEELQVLGQDKLAKISALTRL", "organism": "Arthrospira maxima CS-328", "uniprot_code": "B5W3T4", "uniprot_range_first": 1, "uniprot_range_last": 106, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MSEINWSEQDKNAAVDALHKAYEREVKILIDEVKQKAFNLTEIDEVWKLHDFLSARRHDI\nDGKYDYRDAASVFVLATLVKQGWLSLEELQVLGQDKLAKISALTRL", "mw": 12.244, "total_mw": 24.488, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.6, "deuteration": null }, "purity_method": null, "name": "Arthrospira fluorescence recovery protein AmaxFRP.1-106", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Cy M", "contributor_surname": "Jeffries", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2017-09-01", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 0.05, "number_of_frames": 20, "wavelength": 0.124, "sample_detector_distance": null, "concentration_min": null, "concentration_max": 5.77, "sample_volume": null, "flow_rate": null, "s_min": 0.088, "s_max": 3.886, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDD52_fit1_model1_img.png", "software": "GASBOR", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "2.3i", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDD52_fit1_model1.pdb", "model_mw": null, "bead_radius": 1.9, "log": null, "symmetry": "P2", "comment": null, "user": 9 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD52_fit1_fit_img.png", "software": "GASBOR", "chi_square_value": 1.08, "p_value": 0.48876, "fit_residual_plot": "SASDD52_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDD52_fit1.fir", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Amax4.log", "software_version": "2.3i", "description": null }, { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDD52_fit2_model1_img.png", "software": "iTASSER", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDD52_fit2_model1.pdb", "model_mw": 24.44, "bead_radius": null, "log": null, "symmetry": "P2", "comment": "Fitted using CRYSOL with 25 harmonics, 25 points and constant adjusment.", "user": 9 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD52_fit2_fit_img.png", "software": "CRYSOL", "chi_square_value": 1.127, "p_value": 0.285306, "fit_residual_plot": "SASDD52_fit2_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDD52_fit2.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/AmaxFRP00.log", "software_version": "2.8.3", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": "Additional GASBOR models, with spatial alignments and the NSD estimate can be located in the full entry zip archive.", "experiment_description": "Synchrotron SAXS data from solutions of Arthrospira fluorescence recovery protein AmaxFRP.1-106 in 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH 7.6 were collected at the EMBL-P12 bioSAXS beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5.77 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled for protein concentration.", "tags": [], "intensity_unit": "1/cm", "experimental_mw": 22.0, "experimental_mw_error": null, "guinier_i0_mw": 22.0, "guinier_i0_mw_error": null, "porod_mw": 22.0, "porod_mw_error": null, "pddf_i0": 0.01621, "pddf_i0_error": null, "guinier_i0": 0.0161, "guinier_i0_error": 3e-05, "pddf_rg": 2.786, "pddf_rg_error": null, "guinier_rg": 2.68, "guinier_rg_error": 0.02, "pddf_dmax": 9.5, "pddf_dmax_error": null, "porod_volume": 35.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 144, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDD52_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }