{ "code": "SASDC53", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N.", "publication": { "title": "The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N.", "author_list": "Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH", "journal": "Biophys J", "doi": "10.1016/j.bpj.2017.08.030", "pmid": "29045862", "published_date": "2017 Oct 17" }, "status": "released", "submitted_date": "2017-04-26", "released_date": "2017-10-25" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDC53.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC53.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC53_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC53_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC53_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC53_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDC53.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Colicin N Translocation domain", "short_name": "ColN-T", "sequence": "MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWSWSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASSHHHHHH", "organism": "Escherichia coli", "uniprot_code": "P08083", "uniprot_range_first": 1, "uniprot_range_last": 90, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWSWSNKPHKNDGFHSDG\nSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNSKPGRYISSNPEYSLLA\nKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNWKGPKYNNKLVKRFVSQF\nLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGVKYKNVAKEVANDIKNFHGRNIRSY\nNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAKDMANKIGNLGKAFKVADLAIKVEK\nIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGAVLSFLPISGLAVTALGVIGI\nMTISYLSSFIDANRVSNINNIISSVIR", "mw": 9.965, "total_mw": 9.965, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "50 mM Na-Phosphate 300 mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.6, "deuteration": null }, "purity_method": null, "name": "Colicin N Translocation domain", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Newcastle, UK", "full_name": "Newcastle University", "webpage": "http://www.ncl.ac.uk" } ], "contributor_name": "Alex", "contributor_surname": "Solovyova", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2012-06-29", "storage_temperature": 4.0, "cell_temperature": 4.0, "exposure_time": 2.0, "number_of_frames": 10, "wavelength": 0.15, "sample_detector_distance": 2.85, "concentration_min": 0.4, "concentration_max": 6.3, "sample_volume": null, "flow_rate": null, "s_min": 0.032, "s_max": 4.548, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDC53_fit1_model1_img.png", "software": "EOM/GAJOE", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDC53_fit1_model1.pdb", "model_mw": 10.04, "bead_radius": 1.9, "log": "https://www.sasbdb.org/media/log_files/GA003.log", "symmetry": "", "comment": "", "user": 213 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDC53_fit1_model2_img.png", "software": "EOM/GAJOE", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDC53_fit1_model2.pdb", "model_mw": 9.949, "bead_radius": 1.9, "log": "https://www.sasbdb.org/media/log_files/GA003_9tEwzuh.log", "symmetry": "", "comment": "", "user": 213 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC53_fit1_fixed_fit_img.png", "software": "EOM/GAJOE", "chi_square_value": 1.5, "p_value": 0.000494, "fit_residual_plot": "fit4columns817_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDC53_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/GA003.log", "software_version": "", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": "The results obtained from the bayesapp estimation of distribution functions from small-angle scattering data (http://www.bayesapp.org/) are included in the full entry zip archive.", "experiment_description": "Synchrotron SAXS data from solutions of Colicin N Translocation domain in 50 mM Na-Phosphate 300 mM NaCl, pH 7.6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 6.3 mg/ml were measured at 4°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.", "tags": [ "idp" ], "intensity_unit": null, "experimental_mw": 10.0, "experimental_mw_error": null, "guinier_i0_mw": 18.5, "guinier_i0_mw_error": 0.88, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 23.4, "pddf_i0_error": 0.0777, "guinier_i0": 22.95, "guinier_i0_error": 0.044, "pddf_rg": 2.93, "pddf_rg_error": 0.02, "guinier_rg": 2.76, "guinier_rg_error": 0.15, "pddf_dmax": 11.42, "pddf_dmax_error": 0.25, "porod_volume": 21.82, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 53, "guinier_point_last": 110, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDC53_datloglog_img.png", "symmetry": null, "last_modified": "2021-08-18T17:26:22.657806+02:00", "bragg_peak": [] }