{ "code": "SASDB54", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.", "publication": { "title": "Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.", "author_list": "Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC", "journal": "Arch Biochem Biophys", "doi": "10.1016/j.abb.2016.04.008", "pmid": "27103305", "published_date": "2016 Jun 15" }, "status": "released", "submitted_date": "2016-02-21", "released_date": "2016-08-01" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDB54.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDB54.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB54_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB54_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB54.sascif", "experiment": { "instrument": { "detector": { "type": "20Hz", "name": "Pilatus 300K", "resolution": null }, "name": "Brazilian Synchrotron Light Laboratory", "city": "Campinas", "country": "Brazil", "beamline_name": "SAXS1 Beamline", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Stress-induced protein sti1", "short_name": "Hop/Sti1", "sequence": "MDANELKNEGNKEFSAGRYVEAVNYFSKAIQLDGQNSVLYSNRSACFAAMQKYKDALDDADKCISIKPNW\r\nAKGYVRRGAALHGMRRYDDAIAAYEKGLSVDPSNSGCTQGVKDVQVAKSREARDPIARVFTPEAFRKIQE\r\nNPKLSLLMLQPDYVKMVDTVVRDPSQARLYMEDQRFALTLMYLSGMKIPNDDEDDEEERPSAKAAAEAKA\r\nKEEKKLLTDNEKEAMALKEEGNKLYLSKRFEEALSKYQEAQAKDPKNTLYILNVSAVYFEQRDYEKCITE\r\nCERGIEHGRENHCDYTIVAKLMTRHAFCLQKQKKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVE\r\nEAYIDPEIARQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCI\r\nELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRLRTIMRIQEMASGQSADGDEAAR\r\nRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDPGISVKINKLISAGIIRFGQ", "organism": "Leishmania braziliensis", "uniprot_code": "A4H5F0", "uniprot_range_first": 1, "uniprot_range_last": 547, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MDANELKNEGNKEFSAGRYVEAVNYFSKAIQLDGQNSVLYSNRSACFAAMQKYKDALDDA\nDKCISIKPNWAKGYVRRGAALHGMRRYDDAIAAYEKGLSVDPSNSGCTQGVKDVQVAKSR\nEARDPIARVFTPEAFRKIQENPKLSLLMLQPDYVKMVDTVVRDPSQARLYMEDQRFALTL\nMYLSGMKIPNDDEDDEEERPSAKAAAEAKAKEEKKLLTDNEKEAMALKEEGNKLYLSKRF\nEEALSKYQEAQAKDPKNTLYILNVSAVYFEQRDYEKCITECERGIEHGRENHCDYTIVAK\nLMTRHAFCLQKQKKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVEEAYIDPEIAR\nQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCI\nELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRLRTIMRIQEMASG\nQSADGDEAARRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDPGISVKINKLISA\nGIIRFGQ", "mw": 62.452, "total_mw": 62.452, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol", "concentration_unit": "mM", "comment": null, "additive": "100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol", "concentration": 25.0, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Leishmania braziliensis stress-induced protein sti1 (LbHop), full length construct", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "São Carlos - SP - Brasil", "full_name": "Instituto de Química de São Carlos", "webpage": "" } ], "contributor_name": "Júlio", "contributor_surname": "Borges", "orcid": null } ], "concentration_method": null, "concentration_unit": "mg/ml", "date": "2016-02-21", "storage_temperature": null, "cell_temperature": null, "exposure_time": null, "number_of_frames": null, "wavelength": null, "sample_detector_distance": null, "concentration_min": 0.8, "concentration_max": 1.8, "sample_volume": null, "flow_rate": null, "s_min": 0.123, "s_max": 4.067, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDB54_fit1_model1_img.png", "software": "DAMFILT", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDB54_fit1_model1.pdb", "model_mw": 58.95, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 100 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB54_fit1_fit_img.png", "software": null, "chi_square_value": 3.549, "p_value": 0.0, "fit_residual_plot": "SASDB54_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDB54_fit1.fir", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "X-ray synchrotron radiation scattering data from solutions of stress-induced protein sti1 (Hop/Sti1) in 25 mM Tris 100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol were collected on the SAXS1 and SAXS2 beam lines at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR Image Plate and Pilatus 300k detectors (s = 4π sin θ/λ, where 2θ is the scattering angle). Different solute concentrations in the range 0.80-1.80 mg/ml were measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were merged to yield the final composite scattering curve. ", "tags": [], "intensity_unit": null, "experimental_mw": 63.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 59.0, "porod_mw_error": null, "pddf_i0": 0.0632, "pddf_i0_error": 0.0002, "guinier_i0": 0.063, "guinier_i0_error": null, "pddf_rg": 4.68, "pddf_rg_error": 0.02, "guinier_rg": 4.51, "guinier_rg_error": 0.05, "pddf_dmax": 18.0, "pddf_dmax_error": null, "porod_volume": 94.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 3, "guinier_point_last": 16, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDB54_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }