{ "code": "SASDG55", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states", "publication": { "title": "Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states", "author_list": "Stachowski T, Snell M, Snell E", "journal": "IUCrJ", "doi": "10.1107/S205225251901707X", "pmid": null, "published_date": "2020 Feb 06" }, "status": "released", "submitted_date": "2019-09-04", "released_date": "2020-02-28" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDG55.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDG55.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG55_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG55_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDG55_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG55_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDG55.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus3 X 2M", "resolution": 172.0 }, "name": "Advanced Light Source (ALS)", "city": "Berkeley, CA", "country": "USA", "beamline_name": "12.3.1 (SIBYLS)", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Latency associated peptide", "short_name": "LAP", "sequence": ">sp|P01137|30-278\r\nLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVA\r\nGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVL\r\nLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLS\r\nRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQ\r\nHLQSSRHRAHHHHHH", "organism": "Homo sapiens", "uniprot_code": "P01137", "uniprot_range_first": 30, "uniprot_range_last": 277, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MPPSGLRLLLLLLPLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLA\nSPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEI\nYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWR\nYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFT\nTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYI\nDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQA\nLEPLPIVYYVGRKPKVEQLSNMIVRSCKCS", "mw": 29.228, "total_mw": 58.456, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "phosphate buffered saline 2% glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "Deglycosylated latency associated peptide, LAP (TGFB-1)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "New York, USA", "full_name": "Hauptman-Woodward Medical Research Institute", "webpage": "http://hwi.buffalo.edu/" } ], "contributor_name": "Tim", "contributor_surname": "Stachowski", "orcid": "https://orcid.org/0000-0002-6097-4857" } ], "concentration_method": null, "concentration_unit": null, "date": "2019-04-20", "storage_temperature": 4.0, "cell_temperature": 10.0, "exposure_time": 0.1, "number_of_frames": 100, "wavelength": 0.127, "sample_detector_distance": 2.0, "concentration_min": null, "concentration_max": 1.4, "sample_volume": null, "flow_rate": null, "s_min": 0.109, "s_max": 2.611, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of deglycosylated latency associated peptide (LAP) from the transforming growth factor beta-1 proprotein (TGFB-1) in phosphate buffered saline, 2% glycerol, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.40 mg/ml was measured at 10°C. 100 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 59.1, "experimental_mw_error": null, "guinier_i0_mw": 47.0, "guinier_i0_mw_error": 5.0, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 1.987, "pddf_i0_error": null, "guinier_i0": 1.99, "guinier_i0_error": 0.01, "pddf_rg": 3.55, "pddf_rg_error": null, "guinier_rg": 3.51, "guinier_rg_error": 0.11, "pddf_dmax": 13.0, "pddf_dmax_error": null, "porod_volume": 129.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": null, "guinier_point_last": null, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDG55_datloglog_img.png", "symmetry": null, "last_modified": "2020-02-28T18:01:03.158567+01:00", "bragg_peak": [] }