{ "code": "SASDV55", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.", "publication": { "title": "Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.", "author_list": "Kim C, Yun SR, Lee SJ, Kim SO, Lee H, Choi J, Kim JG, Kim TW, You S, Kosheleva I, Noh T, Baek J, Ihee H", "journal": "Nat Commun", "doi": "10.1038/s41467-024-51461-z", "pmid": "39143073", "published_date": "2024 Aug 14" }, "status": "released", "submitted_date": "2024-07-11", "released_date": "2024-07-27" }, "pddf_data": null, "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDV55.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV55_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV55_kratky_img.png", "pddf_plot": null, "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV55_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDV55.sascif", "experiment": { "instrument": { "detector": { "type": "CCD", "name": "Rayonix SX165", "resolution": 15.0 }, "name": "Pohang Accelerator Laboratory", "city": "Pohang", "country": "South Korea", "beamline_name": "4C", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "non-specific serine/threonine protein kinase", "short_name": null, "sequence": "FLATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL", "organism": "Avena sativa", "uniprot_code": "O49003", "uniprot_range_first": 404, "uniprot_range_last": 546, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MASKGAGGGGGGHEEPQRPKQQLPRDSRGSLEVFNPSSSSAAVEPPSAFRPAARSASPFI\nEEATGGIEDVGKATQRAAEWGLVLQTNEQTGRPQGVSARSSGGGGSARSSSDDKAVAGAI\nPRVSEELRAALSAFQQTFVVSDASRPGHPIMYASAGFFNMTGYTSKEVVGRNCRFLQGSG\nTDPAEIAKIRQALANGSNYCGRVLNYKKDGTAFWNLLTIAPIKDEEGRVLKFIGMQVEVS\nKYTEGNKDTVVRPNGLPESLIKYDARQKDQARSSVSELLLAIKNPRSLSESTNSTFKRKS\nQESVGALTGDRPGKRSSESGSRRNSKSGARTSLQKISEVPERGSKSRKSGLYSLMSLLGM\nGPGNIEKDMLKPRDEDPLLDSDDERPESFDDELRRKEMRRGIDLATTLERIEKNFVITDP\nRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQL\nINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENID\nEAAKELPDANLRPEDLWANHSKVVLPKPHMKDSASWRAIQKVLEGGENIDLKHFRPVKPL\nGSGDTGSVHLVELLNTGEYFAMKAMDKNVMLNRNKVHRANAEREILDMLDHPFLPTLYAS\nFQTKTHICLITDYYPGGELFLLLDRQPLKVLREDAVRFYAAEVVIALEYLHCQGIIYRDL\nKPENILLHRDGHISLTDFDLSCLTSCRPQVFLPEEANKKSRRKSRSSPIFFAEPMRASNS\nFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKDIRF\nPASISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKLDAP\nLFPDDTDKGMGDAAAADTHTDMF", "mw": 16.645, "total_mw": 16.645, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "Tandem PAS-PAC domains." } ], "buffer": { "name": "20 mM Tris, 200 mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.0, "deuteration": null }, "purity_method": null, "name": "The dark-adapted AsLOV2 domain (Avena sativa, Oat)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": null, "full_name": "Korea Advanced Institute of Science and Technology", "webpage": null } ], "contributor_name": "Hyotcherl", "contributor_surname": "Ihee", "orcid": "https://orcid.org/0000-0003-0397-5965" } ], "concentration_method": null, "concentration_unit": null, "date": "2021-05-15", "storage_temperature": 24.0, "cell_temperature": 24.0, "exposure_time": 10.0, "number_of_frames": 50, "wavelength": 0.0734, "sample_detector_distance": 3.0, "concentration_min": null, "concentration_max": 24.98, "sample_volume": null, "flow_rate": null, "s_min": 0.252, "s_max": 5.999, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": null, "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of dark-adapted AsLOV2 domain in 20 mM Tris, 200 mM NaCl, pH 7 were collected on the 4C beam line at the Pohang Accelerator Laboratory (Pohang, South Korea) using a Rayonix SX165 detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.0734 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 24.98 mg/ml was measured at 24°C. 50 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 16.65, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 12.7137, "guinier_i0_error": null, "pddf_rg": null, "pddf_rg_error": null, "guinier_rg": 1.764, "guinier_rg_error": 0.008, "pddf_dmax": 6.6, "pddf_dmax_error": null, "porod_volume": 21.1, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 400, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDV55_datloglog_img.png", "symmetry": null, "last_modified": "2024-10-07T14:53:56.262293+02:00", "bragg_peak": [] }