{
  "code": "SASDD56",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks.",
    "publication": {
      "title": "Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks.",
      "author_list": "Kobayashi N, Inano K, Sasahara K, Sato T, Miyazawa K, Fukuma T, Hecht MH, Song C, Murata K, Arai R",
      "journal": "ACS Synth Biol",
      "doi": "10.1021/acssynbio.8b00007",
      "pmid": "29690759",
      "published_date": "2018 May 18"
    },
    "status": "released",
    "submitted_date": "2018-04-12",
    "released_date": "2018-05-07"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDD56.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD56_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD56_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD56_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDD56.sascif",
  "experiment": {
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        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "Photon Factory (PF), High Energy Accelerator Research Organization (KEK)",
      "city": "Tsukuba",
      "country": "Japan",
      "beamline_name": "BL-10C",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
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          "short_name": "sPN-Block (WA20)",
          "sequence": "MYGKLNKLVEHIKELLQQLNKNWHRHQGNLHDMNQQMEQLFQEFQHFMQGNQDDGKLQNMIHEMQQFMNQVDNHLQSESDTVHHFHNKLQELMNNFHHLVHR",
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          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
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        },
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          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
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          "total_mw": 26.879,
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          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Artificially designed de novo protein esPN-Block (FL4) heterocomplex, e1s2 (FL4)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Japan",
            "full_name": "Shinshu University",
            "webpage": "https://www.shinshu-u.ac.jp"
          }
        ],
        "contributor_name": "Ryoichi",
        "contributor_surname": "Arai",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2014-12-19",
    "storage_temperature": null,
    "cell_temperature": 20.0,
    "exposure_time": 5.0,
    "number_of_frames": 30,
    "wavelength": 0.1488,
    "sample_detector_distance": 1.06,
    "concentration_min": null,
    "concentration_max": 3.5,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.112,
    "s_max": 5.453,
    "total_exposure_time": null,
    "seccolumn": null
  },
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  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of an artificially designed de novo protein, esPN-Block (FL4) heterocomplex e1s2 (FL4), in 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% v/v glycerol, pH 7.5 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Acceleration Research Organization (KEK; Tsukuba, Japan) using a Pilatus 2M detector at a sample-detector distance of 1.1 m and at a wavelength of λ = 0.1488 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was measured at 20°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "1/cm",
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  "pddf_i0_error": 0.00121,
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  "guinier_i0_error": 0.0001,
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  "guinier_rg_error": 0.05,
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  "estimated_volume_error": null,
  "guinier_point_first": 13,
  "guinier_point_last": 42,
  "pddf_point_first": null,
  "pddf_point_last": null,
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  "intensities_log_log_plot": "SASDD56_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-06-15T13:20:35.505343+02:00",
  "bragg_peak": []
}