data_SASDF58_MAIN # _sas_result.id 1476 _sas_result.SASBDB_code SASDF58 _sas_result.experimental_MW 28.900 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 23.000 _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 6172.0000000000 _sas_result.I0_from_Guinier_error 26.0000000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 3.450 _sas_result.Rg_from_Guinier_error 0.300 _sas_result.D_max 14.00 _sas_result.Porod_volume 38.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 19 _sas_result.Guinier_point_max 174 _sas_result.type_of_curve extrapolated _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments "There is a concentration effect for the delta subunit of RNA polymerase in a low salt concentration buffer." # _sas_scan.title "Delta subunit of RNA polymerase, RNAP (B. subtilis), 10mM NaCl" _sas_scan.detc_id 4 _sas_scan.id 1476 _sas_scan.measurement_date 2016-10-03 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 20.0 _sas_scan.exposure_time 0.050 _sas_scan.number_of_frames 20 _sas_scan.unit 1/nm _sas_scan.qmin 0.0134 _sas_scan.qmax 3.7904 _sas_scan.sample_id 1437 _sas_scan.result_id 1476 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.00000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1437 _sas_sample.name "Delta subunit of RNA polymerase, RNAP (B. subtilis), 10mM NaCl" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 0.90-3.60 _sas_sample.concentration_method . _sas_sample.entity_id 1736 # _sas_buffer.id 897 _sas_buffer.name "20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 6.600 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1437 # _entity.id 1736 _entity.type protein _entity.pdbx_description "DNA-directed RNA polymerase subunit delta" _entity.formula_weight 20.399 _entity.pdbx_number_of_molecules 1 # _entity_name_com.entity_id 1736 _entity_name_com.name RpoE # _entity_poly.entity_id 1736 _entity_poly.pdbx_seq_one_letter_code MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK # _entity_src_gen.entity_id 1736 _entity_src_gen.gene_src_common_name "Bacillus subtilis (strain 168)" # _struct_ref.id 1 _struct_ref.entity_id 1736 _struct_ref.db_name UniProt _struct_ref.db_code P12464 _struct_ref.details http://www.uniprot.org/uniprot/P12464 # _citation.id 495 _citation.title "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase." _citation.year "2019 Sep 24" _citation.pdbx_database_id_DOI 10.1021/jacs.9b07837 _citation.pdbx_database_id_PubMed 31550880 # loop_ _citation_author.citation_id _citation_author.ordinal _citation_author.name 495 1 "Kuban V" 495 2 "Srb P" 495 3 "Stegnerova H" 495 4 "Padrta P" 495 5 "Zachrdla M" 495 6 "Jasenakova Z" 495 7 "Šanderová H" 495 8 "Vítovská D" 495 9 "Krasny L" 495 10 "Koval T" 495 11 "Dohnalek J" 495 12 "Ziemska-Legi Cka J" 495 13 "Grynberg M" 495 14 "Jarnot P" 495 15 "Gruca A" 495 16 "Jensen MR" 495 17 "Blackledge M" # _pdbx_contact_author.id 83 _pdbx_contact_author.name_first Vojtěch _pdbx_contact_author.name_last Kubáň _pdbx_contact_author.role . _pdbx_contact_author.address_1 MUNI _pdbx_contact_author.address_2 "Masaryk University; Research group Structure and Dynamics of Proteins." _pdbx_contact_author.address_3 "Brno, Czech Republic" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 1.337960E-02 -9.326386E+02 1.575562E+04 1476 2 1.546980E-02 -8.657180E+03 1.004458E+04 1476 3 1.756010E-02 -6.516786E+03 6.273775E+03 1476 4 1.965030E-02 2.033322E+03 4.242316E+03 1476 5 2.174050E-02 1.259891E+04 3.460652E+03 1476 6 2.383070E-02 8.842858E+03 2.704078E+03 1476 7 2.592090E-02 6.669322E+03 2.411833E+03 1476 8 2.801120E-02 7.620656E+03 1.850134E+03 1476 9 3.010140E-02 6.613364E+03 1.609507E+03 1476 10 3.219160E-02 6.721796E+03 1.402167E+03 1476 11 3.428180E-02 7.010595E+03 1.176541E+03 1476 12 3.637210E-02 6.171667E+03 1.076879E+03 1476 13 3.846230E-02 6.906624E+03 8.930431E+02 1476 14 4.055250E-02 7.048623E+03 8.142273E+02 1476 15 4.264270E-02 7.467588E+03 7.408858E+02 1476 16 4.473290E-02 6.937493E+03 6.551125E+02 1476 17 4.682320E-02 7.133113E+03 6.282782E+02 1476 18 4.891340E-02 7.112846E+03 5.771348E+02 1476 19 5.100360E-02 5.955949E+03 5.676155E+02 1476 20 5.309380E-02 6.053101E+03 5.205502E+02 1476 21 5.518410E-02 6.521990E+03 4.888688E+02 1476 22 5.727430E-02 5.975813E+03 4.716062E+02 1476 23 5.936450E-02 5.939909E+03 4.360563E+02 1476 24 6.145470E-02 6.732291E+03 4.461439E+02 1476 25 6.354490E-02 5.874098E+03 3.910558E+02 1476 26 6.563520E-02 5.700502E+03 3.887977E+02 1476 27 6.772540E-02 6.019177E+03 3.722802E+02 1476 28 6.981560E-02 6.751014E+03 3.666230E+02 1476 29 7.190580E-02 6.442072E+03 3.393052E+02 1476 30 7.399610E-02 5.796905E+03 3.262495E+02 1476 31 7.608630E-02 6.155589E+03 3.257433E+02 1476 32 7.817650E-02 5.936461E+03 3.111348E+02 1476 33 8.026670E-02 6.013218E+03 3.034774E+02 1476 34 8.235690E-02 6.333275E+03 2.914742E+02 1476 35 8.444720E-02 5.856284E+03 2.837713E+02 1476 36 8.653740E-02 5.990386E+03 2.840452E+02 1476 37 8.862760E-02 6.306126E+03 2.680635E+02 1476 38 9.071780E-02 5.775518E+03 2.654942E+02 1476 39 9.280810E-02 6.104570E+03 2.574796E+02 1476 40 9.489830E-02 5.837449E+03 2.610343E+02 1476 41 9.698850E-02 6.156356E+03 2.574199E+02 1476 42 9.907870E-02 5.903883E+03 2.421984E+02 1476 43 1.011690E-01 6.259713E+03 2.464027E+02 1476 44 1.032590E-01 5.670940E+03 2.360895E+02 1476 45 1.053490E-01 5.865298E+03 2.393495E+02 1476 46 1.074400E-01 5.960032E+03 2.283133E+02 1476 47 1.095300E-01 6.225176E+03 2.263161E+02 1476 48 1.116200E-01 6.106442E+03 2.334751E+02 1476 49 1.137100E-01 5.691364E+03 2.200687E+02 1476 50 1.158000E-01 6.189616E+03 2.167788E+02 1476 51 1.178910E-01 6.209331E+03 2.158470E+02 1476 52 1.199810E-01 6.080098E+03 2.122149E+02 1476 53 1.220710E-01 6.179765E+03 2.187184E+02 1476 54 1.241610E-01 5.904873E+03 2.011358E+02 1476 55 1.262520E-01 5.839677E+03 2.037827E+02 1476 56 1.283420E-01 5.421458E+03 2.008839E+02 1476 57 1.304320E-01 5.700742E+03 1.984751E+02 1476 58 1.325220E-01 5.876768E+03 2.015658E+02 1476 59 1.346120E-01 5.807819E+03 1.940351E+02 1476 60 1.367030E-01 5.583051E+03 1.948822E+02 1476 61 1.387930E-01 5.735776E+03 1.895236E+02 1476 62 1.408830E-01 5.622081E+03 1.927729E+02 1476 63 1.429730E-01 5.808050E+03 1.844854E+02 1476 64 1.450640E-01 5.801813E+03 1.862556E+02 1476 65 1.471540E-01 5.876995E+03 1.879521E+02 1476 66 1.492440E-01 5.656965E+03 1.822961E+02 1476 67 1.513340E-01 5.345268E+03 1.785505E+02 1476 68 1.534240E-01 5.726809E+03 1.763627E+02 1476 69 1.555150E-01 5.537819E+03 1.772670E+02 1476 70 1.576050E-01 5.692084E+03 1.796462E+02 1476 71 1.596950E-01 5.504091E+03 1.715638E+02 1476 72 1.617850E-01 5.694538E+03 1.723814E+02 1476 73 1.638760E-01 5.669986E+03 1.696794E+02 1476 74 1.659660E-01 5.362062E+03 1.712259E+02 1476 75 1.680560E-01 4.981021E+03 1.667645E+02 1476 76 1.701460E-01 5.301024E+03 1.639305E+02 1476 77 1.722360E-01 5.296712E+03 1.672108E+02 1476 78 1.743270E-01 5.419208E+03 1.628799E+02 1476 79 1.764170E-01 5.286246E+03 1.641472E+02 1476 80 1.785070E-01 5.144883E+03 1.573080E+02 1476 81 1.805970E-01 5.596777E+03 1.625964E+02 1476 82 1.826880E-01 5.532062E+03 1.626617E+02 1476 83 1.847780E-01 5.530625E+03 1.584834E+02 1476 84 1.868680E-01 5.515257E+03 1.578866E+02 1476 85 1.889580E-01 5.561462E+03 1.552786E+02 1476 86 1.910480E-01 5.155018E+03 1.542161E+02 1476 87 1.931390E-01 5.243456E+03 1.561088E+02 1476 88 1.952290E-01 5.120645E+03 1.492556E+02 1476 89 1.973190E-01 5.261671E+03 1.549233E+02 1476 90 1.994090E-01 5.123200E+03 1.488908E+02 1476 91 2.015000E-01 5.327971E+03 1.527543E+02 1476 92 2.035900E-01 5.144668E+03 1.472079E+02 1476 93 2.056800E-01 5.054045E+03 1.449680E+02 1476 94 2.077700E-01 5.324070E+03 1.529615E+02 1476 95 2.098600E-01 5.061753E+03 1.435587E+02 1476 96 2.119510E-01 4.929673E+03 1.469036E+02 1476 97 2.140410E-01 4.951021E+03 1.414974E+02 1476 98 2.161310E-01 5.096957E+03 1.429030E+02 1476 99 2.182210E-01 5.228714E+03 1.467192E+02 1476 100 2.203120E-01 5.298934E+03 1.425689E+02 1476 101 2.224020E-01 4.864186E+03 1.389244E+02 1476 102 2.244920E-01 4.907419E+03 1.406028E+02 1476 103 2.265820E-01 5.069866E+03 1.405033E+02 1476 104 2.286720E-01 5.164623E+03 1.402370E+02 1476 105 2.307630E-01 4.989478E+03 1.373662E+02 1476 106 2.328530E-01 4.826781E+03 1.376792E+02 1476 107 2.349430E-01 4.851233E+03 1.373687E+02 1476 108 2.370330E-01 4.974992E+03 1.374686E+02 1476 109 2.391240E-01 4.900967E+03 1.367929E+02 1476 110 2.412140E-01 4.619820E+03 1.312037E+02 1476 111 2.433040E-01 4.903132E+03 1.361262E+02 1476 112 2.453940E-01 4.811578E+03 1.330902E+02 1476 113 2.474840E-01 4.902605E+03 1.344715E+02 1476 114 2.495750E-01 4.933220E+03 1.300376E+02 1476 115 2.516650E-01 4.888246E+03 1.325324E+02 1476 116 2.537550E-01 4.921175E+03 1.313986E+02 1476 117 2.558450E-01 4.805649E+03 1.303678E+02 1476 118 2.579360E-01 4.809035E+03 1.294101E+02 1476 119 2.600260E-01 4.754479E+03 1.282508E+02 1476 120 2.621160E-01 4.560749E+03 1.282285E+02 1476 121 2.642060E-01 4.480585E+03 1.267486E+02 1476 122 2.662960E-01 4.659815E+03 1.261905E+02 1476 123 2.683870E-01 4.535585E+03 1.248576E+02 1476 124 2.704770E-01 4.631434E+03 1.243505E+02 1476 125 2.725670E-01 4.388914E+03 1.266963E+02 1476 126 2.746570E-01 4.357226E+03 1.233442E+02 1476 127 2.767480E-01 4.625836E+03 1.231261E+02 1476 128 2.788380E-01 4.506486E+03 1.233094E+02 1476 129 2.809280E-01 4.485613E+03 1.223320E+02 1476 130 2.830180E-01 4.395761E+03 1.223332E+02 1476 131 2.851080E-01 4.583944E+03 1.199040E+02 1476 132 2.871990E-01 4.491798E+03 1.233662E+02 1476 133 2.892890E-01 4.461072E+03 1.199802E+02 1476 134 2.913790E-01 4.449385E+03 1.203425E+02 1476 135 2.934690E-01 4.330105E+03 1.182160E+02 1476 136 2.955600E-01 4.379582E+03 1.171970E+02 1476 137 2.976500E-01 4.385437E+03 1.209229E+02 1476 138 2.997400E-01 4.148769E+03 1.170921E+02 1476 139 3.018300E-01 4.241239E+03 1.170118E+02 1476 140 3.039200E-01 4.399418E+03 1.183891E+02 1476 141 3.060110E-01 4.243979E+03 1.149121E+02 1476 142 3.081010E-01 4.117782E+03 1.182585E+02 1476 143 3.101910E-01 4.283977E+03 1.145614E+02 1476 144 3.122810E-01 4.118825E+03 1.141392E+02 1476 145 3.143720E-01 4.130234E+03 1.143182E+02 1476 146 3.164620E-01 4.189633E+03 1.141531E+02 1476 147 3.185520E-01 4.087502E+03 1.146321E+02 1476 148 3.206420E-01 4.139059E+03 1.105415E+02 1476 149 3.227320E-01 3.954252E+03 1.129581E+02 1476 150 3.248230E-01 4.042370E+03 1.129208E+02 1476 151 3.269130E-01 4.049642E+03 1.127496E+02 1476 152 3.290030E-01 4.575901E+03 1.171170E+02 1476 153 3.310930E-01 3.958099E+03 1.084990E+02 1476 154 3.331840E-01 3.906379E+03 1.116546E+02 1476 155 3.352740E-01 3.778909E+03 1.098000E+02 1476 156 3.373640E-01 4.012345E+03 1.095558E+02 1476 157 3.394540E-01 3.754214E+03 1.081041E+02 1476 158 3.415440E-01 3.871912E+03 1.072584E+02 1476 159 3.436350E-01 4.024078E+03 1.095366E+02 1476 160 3.457250E-01 3.891134E+03 1.065347E+02 1476 161 3.478150E-01 3.859796E+03 1.076987E+02 1476 162 3.499050E-01 3.852490E+03 1.120954E+02 1476 163 3.519960E-01 3.588181E+03 1.145740E+02 1476 164 3.540860E-01 3.711241E+03 1.171136E+02 1476 165 3.561760E-01 3.875264E+03 1.146164E+02 1476 166 3.582660E-01 3.824598E+03 1.188052E+02 1476 167 3.603560E-01 3.718328E+03 1.196504E+02 1476 168 3.624470E-01 3.463675E+03 1.214259E+02 1476 169 3.645370E-01 3.756056E+03 1.207465E+02 1476 170 3.666270E-01 3.500169E+03 1.209399E+02 1476 171 3.687170E-01 3.720314E+03 1.243634E+02 1476 172 3.708080E-01 3.575957E+03 1.227599E+02 1476 173 3.728980E-01 3.735292E+03 1.209349E+02 1476 174 3.749880E-01 3.433850E+03 1.216235E+02 1476 175 3.770780E-01 3.434649E+03 6.992838E+01 1476 176 3.791680E-01 3.552799E+03 7.044724E+01 1476 177 3.812590E-01 3.242442E+03 6.685390E+01 1476 178 3.833490E-01 3.511507E+03 7.058460E+01 1476 179 3.854390E-01 3.378557E+03 6.915167E+01 1476 180 3.875290E-01 3.379473E+03 6.991075E+01 1476 181 3.896200E-01 3.268377E+03 7.024400E+01 1476 182 3.917100E-01 3.226517E+03 6.758307E+01 1476 183 3.938000E-01 3.421136E+03 6.894050E+01 1476 184 3.958900E-01 3.301324E+03 6.453838E+01 1476 185 3.979800E-01 3.196357E+03 6.443364E+01 1476 186 4.000710E-01 3.305389E+03 6.361158E+01 1476 187 4.021610E-01 3.205171E+03 6.239082E+01 1476 188 4.042510E-01 3.236713E+03 6.405870E+01 1476 189 4.063410E-01 3.165399E+03 6.113676E+01 1476 190 4.084320E-01 3.273719E+03 6.208330E+01 1476 191 4.105220E-01 3.087252E+03 6.051931E+01 1476 192 4.126120E-01 3.123040E+03 5.993704E+01 1476 193 4.147020E-01 3.201108E+03 6.167459E+01 1476 194 4.167920E-01 3.196802E+03 5.944156E+01 1476 195 4.188830E-01 3.090277E+03 5.912909E+01 1476 196 4.209730E-01 3.053466E+03 5.881113E+01 1476 197 4.230630E-01 3.106066E+03 5.923989E+01 1476 198 4.251530E-01 2.977169E+03 5.887008E+01 1476 199 4.272440E-01 3.088043E+03 5.781354E+01 1476 200 4.293340E-01 3.085252E+03 5.836930E+01 1476 201 4.314240E-01 3.031561E+03 5.726573E+01 1476 202 4.335140E-01 3.036623E+03 5.786202E+01 1476 203 4.356040E-01 2.960392E+03 5.680305E+01 1476 204 4.376950E-01 3.011632E+03 5.690660E+01 1476 205 4.397850E-01 3.130898E+03 5.718621E+01 1476 206 4.418750E-01 2.974150E+03 5.573678E+01 1476 207 4.439650E-01 2.905063E+03 5.558855E+01 1476 208 4.460560E-01 2.837029E+03 5.534202E+01 1476 209 4.481460E-01 2.917226E+03 5.534531E+01 1476 210 4.502360E-01 2.857498E+03 5.644523E+01 1476 211 4.523260E-01 2.805158E+03 5.384532E+01 1476 212 4.544160E-01 2.791186E+03 5.480444E+01 1476 213 4.565070E-01 2.905616E+03 5.520717E+01 1476 214 4.585970E-01 2.833706E+03 5.448507E+01 1476 215 4.606870E-01 2.804208E+03 5.352956E+01 1476 216 4.627770E-01 2.773822E+03 5.362154E+01 1476 217 4.648680E-01 2.760411E+03 5.379247E+01 1476 218 4.669580E-01 2.773929E+03 5.349535E+01 1476 219 4.690480E-01 2.748449E+03 5.354156E+01 1476 220 4.711380E-01 2.745253E+03 5.255724E+01 1476 221 4.732280E-01 2.655731E+03 5.273378E+01 1476 222 4.753190E-01 2.725240E+03 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