{ "code": "SASDC63", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039.", "publication": { "title": "Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039.", "author_list": "Anasir MI, Caria S, Skinner MA, Kvansakul M", "journal": "J Biol Chem", "doi": "10.1074/jbc.M116.768879", "pmid": "28411240", "published_date": "2017 Jun 2" }, "status": "released", "submitted_date": "2017-04-28", "released_date": "2017-04-29" }, "pddf_data": null, "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC63.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC63_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC63_kratky_img.png", "pddf_plot": null, "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC63_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDC63.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "Australian Synchrotron", "city": "Melbourne", "country": "Australia", "beamline_name": "SAXS/WAXS", "beam_geometry": "Point", "type_of_source": "X-ray synchrotron", "point_source": true, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Bcl-2-like protein FPV039", "short_name": "FPV039", "sequence": "MASSNMKDETYYIALNMIQNYIIEYNTNKPRKSFVIDSISYDVLKAACKSVIKTNYNEFDIIISRNIDFNVIVTQVLEDKINWGRIITIIAFCAYYSKKVKQDTSPQYYDGIISEAITDAILSKYRSWFIDQDYWNGIRIYKN", "organism": "Fowlpox virus", "uniprot_code": "Q9J5G4", "uniprot_range_first": 1, "uniprot_range_last": 143, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MASSNMKDETYYIALNMIQNYIIEYNTNKPRKSFVIDSISYDVLKAACKSVIKTNYNEFD\nIIISRNIDFNVIVTQVLEDKINWGRIITIIAFCAYYSKKVKQDTSPQYYDGIISEAITDA\nILSKYRSWFIDQDYWNGIRIYKNYSYIFNTASYCIFTASLIIASLAVFKICSFYM", "mw": 16.823, "total_mw": 16.823, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Uncharacterized protein (BAK1)", "short_name": "Bak", "sequence": "LGSTGSQVGRRLAIIGDDINKRYDAE", "organism": "Gallus gallus", "uniprot_code": "Q5F404", "uniprot_range_first": 72, "uniprot_range_last": 97, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MEGNEGDPPGAHRRRDSHGRRPSREINAEDQVAQETEEVFRSYTFYRYQQEREEGGAEVP\nMDPEIMEIQQELGSTGSQVGRRLAIIGDDINKRYDAEFRHMLKSLQPTKENAYEYFTTIA\nSSLFDSGINWGRVIALLGFGYCMAIHVYQQGITGFLRRIARYVTEFMLRNRIARWIAQQG\nGWVAALDLDNVYMKYMLVVLALVMVGHLVVRRFFRP", "mw": 2.805, "total_mw": 2.805, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM trisodium citrate pH, 200 mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 6.0, "deuteration": null }, "purity_method": null, "name": "Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide (FPV039:BAK)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "ANSTO", "address": "Kirrawee DC, NSW 2232, Australia", "full_name": "Australian Nuclear Science and Technology Organisation", "webpage": "" } ], "contributor_name": "Sofia", "contributor_surname": "Caria", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2015-10-02", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 1.0, "number_of_frames": 30, "wavelength": null, "sample_detector_distance": 1.6, "concentration_min": 0.31, "concentration_max": 7.35, "sample_volume": null, "flow_rate": null, "s_min": 0.106, "s_max": 6.185, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": null, "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide in 20 mM trisodium citrate, 200 mM NaCl, pH 6.0 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.31 to 7.35 mg/ml were measured. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve (7.35 mg/ml scattering data).", "tags": [], "intensity_unit": null, "experimental_mw": 17.9, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 0.104031, "guinier_i0_error": 0.0002, "pddf_rg": null, "pddf_rg_error": null, "guinier_rg": 1.96, "guinier_rg_error": 0.006, "pddf_dmax": null, "pddf_dmax_error": null, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 38, "guinier_point_last": 70, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDC63_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }