data_SASDF68_MAIN # _sas_result.id 1477 _sas_result.SASBDB_code SASDF68 _sas_result.experimental_MW 33.800 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 35.000 _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 7618.0000000000 _sas_result.I0_from_Guinier_error 27.0000000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 3.930 _sas_result.Rg_from_Guinier_error 0.350 _sas_result.D_max 20.00 _sas_result.Porod_volume 56.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 39 _sas_result.Guinier_point_max 150 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments "No concentration effect was observed in higher salt concentration buffers." # _sas_scan.title "Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl" _sas_scan.detc_id 4 _sas_scan.id 1477 _sas_scan.measurement_date 2016-10-03 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 20.0 _sas_scan.exposure_time 0.050 _sas_scan.number_of_frames 20 _sas_scan.unit 1/nm _sas_scan.qmin 0.0134 _sas_scan.qmax 3.7904 _sas_scan.sample_id 1438 _sas_scan.result_id 1477 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.00000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1438 _sas_sample.name "Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 3.60 _sas_sample.concentration_method . _sas_sample.entity_id 1736 # _sas_buffer.id 898 _sas_buffer.name "20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 6.600 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1438 # _entity.id 1736 _entity.type protein _entity.pdbx_description "DNA-directed RNA polymerase subunit delta" _entity.formula_weight 20.399 _entity.pdbx_number_of_molecules 1 # _entity_name_com.entity_id 1736 _entity_name_com.name RpoE # _entity_poly.entity_id 1736 _entity_poly.pdbx_seq_one_letter_code MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK # _entity_src_gen.entity_id 1736 _entity_src_gen.gene_src_common_name "Bacillus subtilis (strain 168)" # _struct_ref.id 1 _struct_ref.entity_id 1736 _struct_ref.db_name UniProt _struct_ref.db_code P12464 _struct_ref.details http://www.uniprot.org/uniprot/P12464 # _citation.id 495 _citation.title "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase." _citation.year "2019 Sep 24" _citation.pdbx_database_id_DOI 10.1021/jacs.9b07837 _citation.pdbx_database_id_PubMed 31550880 # loop_ _citation_author.citation_id _citation_author.ordinal _citation_author.name 495 1 "Kuban V" 495 2 "Srb P" 495 3 "Stegnerova H" 495 4 "Padrta P" 495 5 "Zachrdla M" 495 6 "Jasenakova Z" 495 7 "Šanderová H" 495 8 "Vítovská D" 495 9 "Krasny L" 495 10 "Koval T" 495 11 "Dohnalek J" 495 12 "Ziemska-Legi Cka J" 495 13 "Grynberg M" 495 14 "Jarnot P" 495 15 "Gruca A" 495 16 "Jensen MR" 495 17 "Blackledge M" # _pdbx_contact_author.id 83 _pdbx_contact_author.name_first Vojtěch _pdbx_contact_author.name_last Kubáň _pdbx_contact_author.role . _pdbx_contact_author.address_1 MUNI _pdbx_contact_author.address_2 "Masaryk University; Research group Structure and Dynamics of Proteins." _pdbx_contact_author.address_3 "Brno, Czech Republic" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 1.337960E-02 2.094700E+04 8.575722E+03 1477 2 1.546980E-02 2.771000E+03 5.512475E+03 1477 3 1.756010E-02 7.850100E+03 3.443765E+03 1477 4 1.965030E-02 1.150770E+04 2.333592E+03 1477 5 2.174050E-02 9.227100E+03 1.901168E+03 1477 6 2.383070E-02 9.013900E+03 1.491242E+03 1477 7 2.592090E-02 1.028250E+04 1.338800E+03 1477 8 2.801120E-02 8.866300E+03 1.029098E+03 1477 9 3.010140E-02 1.049990E+04 9.022908E+02 1477 10 3.219160E-02 9.558610E+03 7.841211E+02 1477 11 3.428180E-02 8.650910E+03 6.639316E+02 1477 12 3.637210E-02 9.439470E+03 6.134392E+02 1477 13 3.846230E-02 8.598820E+03 5.088844E+02 1477 14 4.055250E-02 9.658410E+03 4.722627E+02 1477 15 4.264270E-02 8.691440E+03 4.284821E+02 1477 16 4.473290E-02 8.516520E+03 3.828386E+02 1477 17 4.682320E-02 8.110380E+03 3.679551E+02 1477 18 4.891340E-02 8.475500E+03 3.424092E+02 1477 19 5.100360E-02 8.630920E+03 3.452765E+02 1477 20 5.309380E-02 8.727910E+03 3.195002E+02 1477 21 5.518410E-02 8.303790E+03 2.966049E+02 1477 22 5.727430E-02 8.434530E+03 2.959683E+02 1477 23 5.936450E-02 7.884640E+03 2.715627E+02 1477 24 6.145470E-02 8.025290E+03 2.792198E+02 1477 25 6.354490E-02 8.124862E+03 2.500732E+02 1477 26 6.563520E-02 8.132759E+03 2.487505E+02 1477 27 6.772540E-02 7.892670E+03 2.394486E+02 1477 28 6.981560E-02 8.196793E+03 2.375941E+02 1477 29 7.190580E-02 8.212205E+03 2.219241E+02 1477 30 7.399610E-02 7.668379E+03 2.140872E+02 1477 31 7.608630E-02 7.492197E+03 2.158039E+02 1477 32 7.817650E-02 8.023657E+03 2.091553E+02 1477 33 8.026670E-02 7.705726E+03 2.036418E+02 1477 34 8.235690E-02 7.734271E+03 1.971187E+02 1477 35 8.444720E-02 7.415835E+03 1.915801E+02 1477 36 8.653740E-02 7.679780E+03 1.938945E+02 1477 37 8.862760E-02 7.475833E+03 1.828480E+02 1477 38 9.071780E-02 7.661925E+03 1.832307E+02 1477 39 9.280810E-02 7.530652E+03 1.759301E+02 1477 40 9.489830E-02 7.282606E+03 1.788104E+02 1477 41 9.698850E-02 7.646759E+03 1.792682E+02 1477 42 9.907870E-02 6.904198E+03 1.640651E+02 1477 43 1.011690E-01 7.339648E+03 1.690627E+02 1477 44 1.032590E-01 7.425603E+03 1.643111E+02 1477 45 1.053490E-01 7.186470E+03 1.657503E+02 1477 46 1.074400E-01 7.219224E+03 1.584235E+02 1477 47 1.095300E-01 6.849920E+03 1.555086E+02 1477 48 1.116200E-01 7.050414E+03 1.634612E+02 1477 49 1.137100E-01 7.293372E+03 1.563701E+02 1477 50 1.158000E-01 7.351361E+03 1.538045E+02 1477 51 1.178910E-01 7.298298E+03 1.517840E+02 1477 52 1.199810E-01 7.007904E+03 1.494993E+02 1477 53 1.220710E-01 6.964516E+03 1.519829E+02 1477 54 1.241610E-01 7.247489E+03 1.444458E+02 1477 55 1.262520E-01 7.286416E+03 1.455758E+02 1477 56 1.283420E-01 6.857436E+03 1.425755E+02 1477 57 1.304320E-01 7.043832E+03 1.418981E+02 1477 58 1.325220E-01 6.738446E+03 1.414911E+02 1477 59 1.346120E-01 7.221023E+03 1.399717E+02 1477 60 1.367030E-01 7.085405E+03 1.398045E+02 1477 61 1.387930E-01 6.813677E+03 1.340419E+02 1477 62 1.408830E-01 6.936275E+03 1.368009E+02 1477 63 1.429730E-01 6.488812E+03 1.295943E+02 1477 64 1.450640E-01 6.890579E+03 1.325471E+02 1477 65 1.471540E-01 6.818178E+03 1.338463E+02 1477 66 1.492440E-01 6.960450E+03 1.307314E+02 1477 67 1.513340E-01 6.769496E+03 1.283964E+02 1477 68 1.534240E-01 6.749351E+03 1.262987E+02 1477 69 1.555150E-01 6.669194E+03 1.274373E+02 1477 70 1.576050E-01 6.650392E+03 1.280325E+02 1477 71 1.596950E-01 6.866712E+03 1.248420E+02 1477 72 1.617850E-01 6.630970E+03 1.236227E+02 1477 73 1.638760E-01 6.737181E+03 1.223336E+02 1477 74 1.659660E-01 6.854878E+03 1.245109E+02 1477 75 1.680560E-01 6.282930E+03 1.200127E+02 1477 76 1.701460E-01 6.634044E+03 1.190976E+02 1477 77 1.722360E-01 6.774783E+03 1.228381E+02 1477 78 1.743270E-01 6.569130E+03 1.171733E+02 1477 79 1.764170E-01 6.434323E+03 1.178698E+02 1477 80 1.785070E-01 6.448578E+03 1.130196E+02 1477 81 1.805970E-01 6.300893E+03 1.153626E+02 1477 82 1.826880E-01 6.472930E+03 1.173747E+02 1477 83 1.847780E-01 6.510364E+03 1.137200E+02 1477 84 1.868680E-01 6.150614E+03 1.120546E+02 1477 85 1.889580E-01 6.349032E+03 1.108695E+02 1477 86 1.910480E-01 6.274822E+03 1.106266E+02 1477 87 1.931390E-01 6.420650E+03 1.125166E+02 1477 88 1.952290E-01 6.354854E+03 1.087826E+02 1477 89 1.973190E-01 6.098548E+03 1.104476E+02 1477 90 1.994090E-01 6.080058E+03 1.066552E+02 1477 91 2.015000E-01 6.214400E+03 1.091770E+02 1477 92 2.035900E-01 6.038768E+03 1.058653E+02 1477 93 2.056800E-01 6.157488E+03 1.048321E+02 1477 94 2.077700E-01 6.125505E+03 1.085367E+02 1477 95 2.098600E-01 6.083635E+03 1.036284E+02 1477 96 2.119510E-01 6.011665E+03 1.060947E+02 1477 97 2.140410E-01 5.861992E+03 1.014706E+02 1477 98 2.161310E-01 5.840646E+03 1.009805E+02 1477 99 2.182210E-01 5.898969E+03 1.042452E+02 1477 100 2.203120E-01 5.675220E+03 9.943420E+01 1477 101 2.224020E-01 5.941984E+03 1.005857E+02 1477 102 2.244920E-01 5.803258E+03 1.002796E+02 1477 103 2.265820E-01 5.773890E+03 9.878318E+01 1477 104 2.286720E-01 5.636306E+03 9.818427E+01 1477 105 2.307630E-01 5.798582E+03 9.826127E+01 1477 106 2.328530E-01 5.838457E+03 9.897621E+01 1477 107 2.349430E-01 5.571835E+03 9.683887E+01 1477 108 2.370330E-01 5.731337E+03 9.801032E+01 1477 109 2.391240E-01 5.799877E+03 9.706093E+01 1477 110 2.412140E-01 5.631669E+03 9.381486E+01 1477 111 2.433040E-01 5.429899E+03 9.516662E+01 1477 112 2.453940E-01 5.569321E+03 9.464707E+01 1477 113 2.474840E-01 5.545867E+03 9.504751E+01 1477 114 2.495750E-01 5.464664E+03 9.211952E+01 1477 115 2.516650E-01 5.461002E+03 9.322015E+01 1477 116 2.537550E-01 5.371529E+03 9.198371E+01 1477 117 2.558450E-01 5.217272E+03 9.146335E+01 1477 118 2.579360E-01 5.384446E+03 9.127694E+01 1477 119 2.600260E-01 5.385747E+03 9.046647E+01 1477 120 2.621160E-01 5.363126E+03 9.093884E+01 1477 121 2.642060E-01 5.289229E+03 8.958745E+01 1477 122 2.662960E-01 5.217248E+03 8.864601E+01 1477 123 2.683870E-01 5.134487E+03 8.825042E+01 1477 124 2.704770E-01 5.148651E+03 8.690090E+01 1477 125 2.725670E-01 5.103807E+03 8.862686E+01 1477 126 2.746570E-01 5.233481E+03 8.727989E+01 1477 127 2.767480E-01 5.137899E+03 8.599627E+01 1477 128 2.788380E-01 5.154385E+03 8.696075E+01 1477 129 2.809280E-01 5.012491E+03 8.533545E+01 1477 130 2.830180E-01 4.868034E+03 8.538995E+01 1477 131 2.851080E-01 4.977502E+03 8.359807E+01 1477 132 2.871990E-01 5.001052E+03 8.600547E+01 1477 133 2.892890E-01 5.001289E+03 8.376527E+01 1477 134 2.913790E-01 5.047588E+03 8.388097E+01 1477 135 2.934690E-01 5.006061E+03 8.353964E+01 1477 136 2.955600E-01 4.789757E+03 8.079745E+01 1477 137 2.976500E-01 4.845484E+03 8.387170E+01 1477 138 2.997400E-01 4.744078E+03 8.155757E+01 1477 139 3.018300E-01 4.710304E+03 8.108200E+01 1477 140 3.039200E-01 4.859299E+03 8.214798E+01 1477 141 3.060110E-01 4.804064E+03 7.976930E+01 1477 142 3.081010E-01 4.711193E+03 8.193872E+01 1477 143 3.101910E-01 4.789577E+03 7.993978E+01 1477 144 3.122810E-01 4.623833E+03 7.979118E+01 1477 145 3.143720E-01 4.662224E+03 7.989195E+01 1477 146 3.164620E-01 4.612456E+03 7.842254E+01 1477 147 3.185520E-01 4.555159E+03 7.911519E+01 1477 148 3.206420E-01 4.531027E+03 7.614602E+01 1477 149 3.227320E-01 4.430139E+03 7.770192E+01 1477 150 3.248230E-01 4.469087E+03 7.746487E+01 1477 151 3.269130E-01 4.564068E+03 7.768745E+01 1477 152 3.290030E-01 4.591446E+03 7.928690E+01 1477 153 3.310930E-01 4.361900E+03 7.460363E+01 1477 154 3.331840E-01 4.457233E+03 7.719765E+01 1477 155 3.352740E-01 4.448314E+03 7.597638E+01 1477 156 3.373640E-01 4.310544E+03 7.462124E+01 1477 157 3.394540E-01 4.314949E+03 7.477656E+01 1477 158 3.415440E-01 4.298420E+03 7.410082E+01 1477 159 3.436350E-01 4.383927E+03 7.576971E+01 1477 160 3.457250E-01 4.197476E+03 7.306057E+01 1477 161 3.478150E-01 4.181166E+03 7.360274E+01 1477 162 3.499050E-01 4.209757E+03 7.697908E+01 1477 163 3.519960E-01 4.151925E+03 7.868045E+01 1477 164 3.540860E-01 4.191670E+03 8.059907E+01 1477 165 3.561760E-01 4.082011E+03 7.799794E+01 1477 166 3.582660E-01 4.035905E+03 8.084221E+01 1477 167 3.603560E-01 4.027660E+03 8.155256E+01 1477 168 3.624470E-01 4.226076E+03 8.408865E+01 1477 169 3.645370E-01 3.979608E+03 8.238444E+01 1477 170 3.666270E-01 4.067898E+03 8.311549E+01 1477 171 3.687170E-01 3.944253E+03 8.442744E+01 1477 172 3.708080E-01 3.955214E+03 8.359912E+01 1477 173 3.728980E-01 3.826721E+03 8.179437E+01 1477 174 3.749880E-01 3.723837E+03 8.171265E+01 1477 175 3.770780E-01 3.922880E+03 8.372552E+01 1477 176 3.791680E-01 3.904094E+03 8.390280E+01 1477 177 3.812590E-01 3.863805E+03 8.043262E+01 1477 178 3.833490E-01 3.838828E+03 8.354954E+01 1477 179 3.854390E-01 3.766955E+03 8.229339E+01 1477 180 3.875290E-01 3.745294E+03 8.283028E+01 1477 181 3.896200E-01 3.673302E+03 8.366089E+01 1477 182 3.917100E-01 3.659700E+03 8.067413E+01 1477 183 3.938000E-01 3.597097E+03 8.057716E+01 1477 184 3.958900E-01 3.688815E+03 7.670871E+01 1477 185 3.979800E-01 3.633607E+03 7.679135E+01 1477 186 4.000710E-01 3.608924E+03 7.536486E+01 1477 187 4.021610E-01 3.642394E+03 7.413838E+01 1477 188 4.042510E-01 3.514570E+03 7.462002E+01 1477 189 4.063410E-01 3.630723E+03 7.280091E+01 1477 190 4.084320E-01 3.497727E+03 7.276748E+01 1477 191 4.105220E-01 3.535956E+03 7.193099E+01 1477 192 4.126120E-01 3.551830E+03 7.078256E+01 1477 193 4.147020E-01 3.394672E+03 7.189435E+01 1477 194 4.167920E-01 3.367983E+03 6.924360E+01 1477 195 4.188830E-01 3.466523E+03 6.997323E+01 1477 196 4.209730E-01 3.411637E+03 6.929109E+01 1477 197 4.230630E-01 3.365258E+03 6.916414E+01 1477 198 4.251530E-01 3.290886E+03 6.872710E+01 1477 199 4.272440E-01 3.527614E+03 6.867183E+01 1477 200 4.293340E-01 3.370315E+03 6.864719E+01 1477 201 4.314240E-01 3.353102E+03 6.736702E+01 1477 202 4.335140E-01 3.415906E+03 6.830074E+01 1477 203 4.356040E-01 3.278423E+03 6.618445E+01 1477 204 4.376950E-01 3.176729E+03 6.603221E+01 1477 205 4.397850E-01 3.393145E+03 6.752464E+01 1477 206 4.418750E-01 3.158834E+03 6.542021E+01 1477 207 4.439650E-01 3.206358E+03 6.509251E+01 1477 208 4.460560E-01 3.228647E+03 6.502385E+01 1477 209 4.481460E-01 3.077433E+03 6.449941E+01 1477 210 4.502360E-01 3.132906E+03 6.573121E+01 1477 211 4.523260E-01 3.009298E+03 6.240672E+01 1477 212 4.544160E-01 3.024404E+03 6.372890E+01 1477 213 4.565070E-01 3.138047E+03 6.422406E+01 1477 214 4.585970E-01 2.929729E+03 6.302745E+01 1477 215 4.606870E-01 3.018329E+03 6.212026E+01 1477 216 4.627770E-01 3.032523E+03 6.235395E+01 1477 217 4.648680E-01 3.103584E+03 6.322380E+01 1477 218 4.669580E-01 2.987319E+03 6.200319E+01 1477 219 4.690480E-01 2.957437E+03 6.269370E+01 1477 220 4.711380E-01 2.940078E+03 6.053596E+01 1477 221 4.732280E-01 2.983539E+03 6.189678E+01 1477 222 4.753190E-01 2.855343E+03 6.089085E+01 1477 223 4.774090E-01 2.989974E+03 6.138092E+01 1477 224 4.794990E-01 2.805256E+03 5.991607E+01 1477 225 4.815890E-01 2.872272E+03 5.984941E+01 1477 226 4.836800E-01 2.928853E+03 6.114407E+01 1477 227 4.857700E-01 2.757662E+03 5.947864E+01 1477 228 4.878600E-01 2.705451E+03 5.894428E+01 1477 229 4.899500E-01 2.769207E+03 5.954051E+01 1477 230 4.920400E-01 2.783436E+03 5.898586E+01 1477 231 4.941310E-01 2.804999E+03 5.933449E+01 1477 232 4.962210E-01 2.674589E+03 5.823195E+01 1477 233 4.983110E-01 2.725986E+03 5.773772E+01 1477 234 5.004010E-01 2.739457E+03 5.819035E+01 1477 235 5.024920E-01 2.734596E+03 5.768629E+01 1477 236 5.045820E-01 2.736651E+03 5.782149E+01 1477 237 5.066720E-01 2.711316E+03 5.663255E+01 1477 238 5.087620E-01 2.663907E+03 5.686922E+01 1477 239 5.108520E-01 2.612141E+03 5.637979E+01 1477 240 5.129430E-01 2.584424E+03 5.575101E+01 1477 241 5.150330E-01 2.559698E+03 5.578056E+01 1477 242 5.171230E-01 2.619530E+03 5.563737E+01 1477 243 5.192130E-01 2.536201E+03 5.563953E+01 1477 244 5.213040E-01 2.551695E+03 5.573297E+01 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