data_SASDC69_MAIN # _sas_result.id 741 _sas_result.SASBDB_code SASDC69 _sas_result.experimental_MW 49.700 _sas_result.experimental_MW_error . _sas_result.MW_standard 49.700 _sas_result.MW_standard_error 0.060 _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 0.1450000000 _sas_result.I0_from_Guinier_error 0.0018000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 2.910 _sas_result.Rg_from_Guinier_error 0.006 _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 1 _sas_result.Guinier_point_max 63 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments "Based on the affinity of the Rev7 dimer, at this concentration (and below) there is a mixture of monomer and dimer in the solution. This dataset was not used for modeling" # _sas_scan.title "Human Rev7 dimer in complex with Rev3 peptide @ 4.6mg/mL" _sas_scan.detc_id 12 _sas_scan.id 741 _sas_scan.measurement_date 2016-05-14 _sas_scan.storage_temperature 4.0 _sas_scan.cell_temperature 4.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 10 _sas_scan.unit 1/A _sas_scan.qmin 0.0086 _sas_scan.qmax 0.2729 _sas_scan.sample_id 701 _sas_scan.result_id 741 _sas_scan.beam_id 23 # _sas_beam.id 23 _sas_beam.instrument_name "Cornell High Energy Synchrotron Source (CHESS) G1" _sas_beam.instrument_city "Ithaca, NY" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.126700 _sas_beam.dist_spec_to_detc . # _sas_detc.id 12 _sas_detc.name "Finger Lakes CCD" _sas_detc.type CCD _sas_detc.pixsize_x . # _sas_sample.id 701 _sas_sample.name "Human Rev7 dimer in complex with Rev3 peptide @ 4.6mg/mL" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 4.60 _sas_sample.concentration_method . loop_ _sas_sample.entity_id 857 858 859 # _sas_buffer.id 426 _sas_buffer.name "20 mM HEPES, 10 mM DTT, 5% glycerol" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 8.000 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 701 # loop_ _entity.id _entity.type _entity.pdbx_description _entity.formula_weight _entity.pdbx_number_of_molecules 857 protein "Mitotic spindle assembly checkpoint protein MAD2B" 24.391 2 858 protein "DNA polymerase zeta catalytic subunit" 3.273 1 859 protein "DNA polymerase zeta catalytic subunit" 3.273 1 # loop_ _entity_name_com.entity_id _entity_name_com.name 857 "hRev7-WT dimer" 858 Rev3-RBM2 859 Rev3-RBM2 # loop_ _entity_poly.entity_id _entity_poly.pdbx_seq_one_letter_code 857 ;GMTTLTRQDLNFGQVVADVLCEFLEVAVHLILYVREVYPVGIFQKRKKYNVPVQMSCHPEL NQYIQDTLHCVKPLLEKNDVEKVVVVILDKEHRPVEKFVFEITQPPLLSISSDSLLSHVE QLLRAFILKISVCDAVLDHNPPGCTFTVLVHTREAATRNMEKIQVIKDFPWILADEQDVH MHDPRLIPLKTMTSDILKMQLYVEERAHKGS ; 858 "MEDK KIVIMPCKCA PSRQLVQVWL QAKE" 859 "MEDK KIVIMPCKCAPSRQLVQVWL QAKE" # loop_ _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name 857 "Homo sapiens" 858 "Homo sapiens" 859 "Homo sapiens" # loop_ _struct_ref.id _struct_ref.entity_id _struct_ref.db_name _struct_ref.db_code _struct_ref.details 1 857 UniProt Q9UI95 http://www.uniprot.org/uniprot/Q9UI95 2 858 UniProt O60673 http://www.uniprot.org/uniprot/O60673 3 859 UniProt O60673 http://www.uniprot.org/uniprot/O60673 # _citation.id 454 _citation.title "Rev7 dimerization is important for assembly and function of the Rev1/PolĪ¶ translesion synthesis complex." _citation.year "2018 Aug 28" _citation.pdbx_database_id_DOI 10.1073/pnas.1801149115 _citation.pdbx_database_id_PubMed 30111544 _citation.journal_abbrev "Proc Natl Acad Sci U S A" _citation.journal_volume 115 _citation.journal_issue 35 # loop_ _citation_author.citation_id _citation_author.ordinal _citation_author.name 454 1 "Rizzo AA" 454 2 "Vassel FM" 454 3 "Chatterjee N" 454 4 "D'Souza S" 454 5 "Li Y" 454 6 "Hao B" 454 7 "Hemann MT" 454 8 "Walker GC" # _pdbx_contact_author.id 196 _pdbx_contact_author.name_first Alex _pdbx_contact_author.name_last Rizzo _pdbx_contact_author.role . _pdbx_contact_author.address_1 . _pdbx_contact_author.address_2 "University of Connecticut" _pdbx_contact_author.address_3 "Storrs, CT, USA" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 8.592058E-03 1.470950E-01 1.892844E-03 741 2 9.164860E-03 1.453789E-01 1.803691E-03 741 3 9.737662E-03 1.420531E-01 1.659034E-03 741 4 1.031046E-02 1.422575E-01 1.397068E-03 741 5 1.088327E-02 1.418878E-01 1.207211E-03 741 6 1.145607E-02 1.418894E-01 1.121170E-03 741 7 1.202887E-02 1.405900E-01 1.083587E-03 741 8 1.260167E-02 1.404769E-01 1.011680E-03 741 9 1.317447E-02 1.394436E-01 9.616209E-04 741 10 1.374727E-02 1.377712E-01 9.276769E-04 741 11 1.432007E-02 1.392992E-01 9.192061E-04 741 12 1.489287E-02 1.363837E-01 9.196594E-04 741 13 1.546567E-02 1.364456E-01 8.724985E-04 741 14 1.603846E-02 1.329257E-01 8.161315E-04 741 15 1.661126E-02 1.347975E-01 8.096206E-04 741 16 1.718406E-02 1.328251E-01 7.582481E-04 741 17 1.775685E-02 1.328131E-01 8.089353E-04 741 18 1.832965E-02 1.320149E-01 7.207949E-04 741 19 1.890245E-02 1.309304E-01 7.070108E-04 741 20 1.947524E-02 1.302623E-01 6.902509E-04 741 21 2.004803E-02 1.295845E-01 6.814739E-04 741 22 2.062083E-02 1.294412E-01 6.808732E-04 741 23 2.119362E-02 1.287054E-01 6.882306E-04 741 24 2.176641E-02 1.271315E-01 6.579621E-04 741 25 2.233921E-02 1.259804E-01 6.280680E-04 741 26 2.291200E-02 1.244136E-01 6.036446E-04 741 27 2.348479E-02 1.251454E-01 6.476425E-04 741 28 2.405758E-02 1.228312E-01 5.780564E-04 741 29 2.463037E-02 1.233538E-01 5.680148E-04 741 30 2.520315E-02 1.205536E-01 5.314306E-04 741 31 2.577594E-02 1.203551E-01 5.061523E-04 741 32 2.634873E-02 1.185746E-01 4.840146E-04 741 33 2.692151E-02 1.174960E-01 4.747062E-04 741 34 2.749430E-02 1.169021E-01 4.738994E-04 741 35 2.806708E-02 1.153673E-01 4.570494E-04 741 36 2.863987E-02 1.148960E-01 4.489816E-04 741 37 2.921265E-02 1.135481E-01 4.341577E-04 741 38 2.978543E-02 1.129588E-01 4.044424E-04 741 39 3.035821E-02 1.123900E-01 4.119532E-04 741 40 3.093099E-02 1.108147E-01 4.138550E-04 741 41 3.150377E-02 1.088677E-01 4.066256E-04 741 42 3.207655E-02 1.079101E-01 4.019999E-04 741 43 3.264933E-02 1.071874E-01 3.954265E-04 741 44 3.322210E-02 1.058407E-01 3.816087E-04 741 45 3.379488E-02 1.045285E-01 3.846804E-04 741 46 3.436765E-02 1.042177E-01 3.795088E-04 741 47 3.494042E-02 1.026092E-01 3.887252E-04 741 48 3.551320E-02 1.020159E-01 3.753643E-04 741 49 3.608597E-02 1.003933E-01 3.724131E-04 741 50 3.665874E-02 9.902117E-02 3.739294E-04 741 51 3.723150E-02 9.770785E-02 3.573819E-04 741 52 3.780427E-02 9.661344E-02 3.518821E-04 741 53 3.837704E-02 9.532066E-02 3.597005E-04 741 54 3.894980E-02 9.458269E-02 3.522915E-04 741 55 3.952257E-02 9.333543E-02 3.472727E-04 741 56 4.009533E-02 9.218723E-02 3.513229E-04 741 57 4.066809E-02 9.092489E-02 3.407416E-04 741 58 4.124085E-02 8.969331E-02 3.457182E-04 741 59 4.181361E-02 8.864988E-02 3.390897E-04 741 60 4.238637E-02 8.749498E-02 3.318715E-04 741 61 4.295913E-02 8.657038E-02 3.273897E-04 741 62 4.353188E-02 8.583590E-02 3.246797E-04 741 63 4.410464E-02 8.443862E-02 3.257721E-04 741 64 4.467739E-02 8.309501E-02 3.172253E-04 741 65 4.525014E-02 8.209679E-02 3.091706E-04 741 66 4.582289E-02 8.112807E-02 3.095960E-04 741 67 4.639564E-02 7.985194E-02 3.020526E-04 741 68 4.696839E-02 7.877208E-02 2.999081E-04 741 69 4.754113E-02 7.791961E-02 2.988913E-04 741 70 4.811388E-02 7.597670E-02 2.927993E-04 741 71 4.868662E-02 7.543899E-02 2.950250E-04 741 72 4.925936E-02 7.427459E-02 2.795302E-04 741 73 4.983210E-02 7.243853E-02 2.827241E-04 741 74 5.040484E-02 7.224791E-02 2.936737E-04 741 75 5.097758E-02 7.130538E-02 2.854112E-04 741 76 5.155031E-02 7.023034E-02 2.809000E-04 741 77 5.212305E-02 6.909889E-02 2.728723E-04 741 78 5.269578E-02 6.814164E-02 2.702269E-04 741 79 5.326851E-02 6.731114E-02 2.730626E-04 741 80 5.384124E-02 6.568437E-02 2.657710E-04 741 81 5.441397E-02 6.518858E-02 2.663711E-04 741 82 5.498669E-02 6.400228E-02 2.704534E-04 741 83 5.555942E-02 6.386468E-02 2.695567E-04 741 84 5.613214E-02 6.198623E-02 2.859920E-04 741 85 5.670486E-02 6.187281E-02 2.800216E-04 741 86 5.727758E-02 6.041340E-02 2.784651E-04 741 87 5.785030E-02 5.928654E-02 2.880681E-04 741 88 5.842302E-02 5.811925E-02 2.782475E-04 741 89 5.899573E-02 5.731965E-02 2.861811E-04 741 90 5.956844E-02 5.682315E-02 2.841212E-04 741 91 6.014115E-02 5.549052E-02 2.804848E-04 741 92 6.071386E-02 5.399212E-02 2.943195E-04 741 93 6.128657E-02 5.339059E-02 2.803866E-04 741 94 6.185927E-02 5.192971E-02 2.758832E-04 741 95 6.243198E-02 5.150269E-02 2.831855E-04 741 96 6.300468E-02 5.043493E-02 2.797569E-04 741 97 6.357738E-02 4.989752E-02 2.843402E-04 741 98 6.415007E-02 4.824889E-02 2.712269E-04 741 99 6.472277E-02 4.764530E-02 2.637505E-04 741 100 6.529546E-02 4.746494E-02 2.639358E-04 741 101 6.586816E-02 4.632551E-02 2.676926E-04 741 102 6.644085E-02 4.574938E-02 2.714111E-04 741 103 6.701353E-02 4.470840E-02 2.682001E-04 741 104 6.758622E-02 4.352955E-02 2.779375E-04 741 105 6.815890E-02 4.298409E-02 2.711040E-04 741 106 6.873158E-02 4.178406E-02 2.712189E-04 741 107 6.930426E-02 4.161903E-02 2.652990E-04 741 108 6.987694E-02 4.035776E-02 2.678653E-04 741 109 7.044962E-02 4.029234E-02 2.626298E-04 741 110 7.102229E-02 3.946787E-02 2.652893E-04 741 111 7.159496E-02 3.860388E-02 2.605548E-04 741 112 7.216763E-02 3.759652E-02 2.582964E-04 741 113 7.274030E-02 3.704352E-02 2.620932E-04 741 114 7.331296E-02 3.625509E-02 2.669675E-04 741 115 7.388562E-02 3.586147E-02 2.579537E-04 741 116 7.445828E-02 3.483035E-02 2.603552E-04 741 117 7.503094E-02 3.417837E-02 2.647032E-04 741 118 7.560360E-02 3.363080E-02 2.605399E-04 741 119 7.617625E-02 3.274807E-02 2.597180E-04 741 120 7.674890E-02 3.239640E-02 2.537472E-04 741 121 7.732155E-02 3.176969E-02 2.568988E-04 741 122 7.789420E-02 3.088930E-02 2.517755E-04 741 123 7.846684E-02 3.045800E-02 2.541505E-04 741 124 7.903948E-02 2.957969E-02 2.466230E-04 741 125 7.961212E-02 2.935780E-02 2.457668E-04 741 126 8.018476E-02 2.882905E-02 2.531963E-04 741 127 8.075740E-02 2.831433E-02 2.477418E-04 741 128 8.133003E-02 2.781425E-02 2.495491E-04 741 129 8.190266E-02 2.702687E-02 2.482197E-04 741 130 8.247528E-02 2.663379E-02 2.439134E-04 741 131 8.304791E-02 2.613399E-02 2.433698E-04 741 132 8.362053E-02 2.574612E-02 2.432405E-04 741 133 8.419315E-02 2.459932E-02 2.411860E-04 741 134 8.476577E-02 2.404070E-02 2.419110E-04 741 135 8.533838E-02 2.397879E-02 2.406298E-04 741 136 8.591100E-02 2.375157E-02 2.416439E-04 741 137 8.648361E-02 2.252450E-02 2.387272E-04 741 138 8.705621E-02 2.221331E-02 2.345865E-04 741 139 8.762882E-02 2.192242E-02 2.401273E-04 741 140 8.820142E-02 2.160935E-02 2.353935E-04 741 141 8.877402E-02 2.080987E-02 2.352777E-04 741 142 8.934661E-02 2.064068E-02 2.379402E-04 741 143 8.991921E-02 1.998878E-02 2.374865E-04 741 144 9.049180E-02 1.971591E-02 2.297549E-04 741 145 9.106439E-02 1.969802E-02 2.370847E-04 741 146 9.163697E-02 1.876387E-02 2.342335E-04 741 147 9.220956E-02 1.862430E-02 2.354958E-04 741 148 9.278214E-02 1.827028E-02 2.264873E-04 741 149 9.335471E-02 1.768958E-02 2.350925E-04 741 150 9.392729E-02 1.727485E-02 2.269906E-04 741 151 9.449986E-02 1.710028E-02 2.255356E-04 741 152 9.507243E-02 1.691646E-02 2.245013E-04 741 153 9.564500E-02 1.631902E-02 2.283950E-04 741 154 9.621756E-02 1.604278E-02 2.287506E-04 741 155 9.679012E-02 1.615809E-02 2.252088E-04 741 156 9.736268E-02 1.540733E-02 2.236251E-04 741 157 9.793523E-02 1.517289E-02 2.271957E-04 741 158 9.850778E-02 1.477271E-02 2.176961E-04 741 159 9.908033E-02 1.449889E-02 2.160774E-04 741 160 9.965288E-02 1.427570E-02 2.080391E-04 741 161 1.002254E-01 1.376874E-02 2.073791E-04 741 162 1.007980E-01 1.339804E-02 2.086776E-04 741 163 1.013705E-01 1.321347E-02 2.051249E-04 741 164 1.019430E-01 1.308978E-02 2.158506E-04 741 165 1.025156E-01 1.298647E-02 2.094898E-04 741 166 1.030881E-01 1.235267E-02 2.119854E-04 741 167 1.036606E-01 1.234361E-02 2.111949E-04 741 168 1.042331E-01 1.157909E-02 2.104601E-04 741 169 1.048057E-01 1.164477E-02 2.099116E-04 741 170 1.053782E-01 1.147116E-02 2.121329E-04 741 171 1.059507E-01 1.100641E-02 2.113995E-04 741 172 1.065232E-01 1.142800E-02 2.087599E-04 741 173 1.070957E-01 1.075999E-02 2.100938E-04 741 174 1.076682E-01 1.046308E-02 2.101006E-04 741 175 1.082407E-01 1.017767E-02 2.048211E-04 741 176 1.088132E-01 1.003380E-02 2.104660E-04 741 177 1.093857E-01 9.510148E-03 2.109456E-04 741 178 1.099582E-01 8.849315E-03 2.038023E-04 741 179 1.105307E-01 9.692434E-03 2.057428E-04 741 180 1.111031E-01 8.690643E-03 2.052044E-04 741 181 1.116756E-01 8.910253E-03 2.106763E-04 741 182 1.122481E-01 8.517342E-03 1.997947E-04 741 183 1.128206E-01 8.324176E-03 2.034068E-04 741 184 1.133930E-01 8.281643E-03 2.020233E-04 741 185 1.139655E-01 8.297420E-03 2.052964E-04 741 186 1.145380E-01 7.953854E-03 2.086640E-04 741 187 1.151104E-01 7.922692E-03 2.046347E-04 741 188 1.156829E-01 7.752167E-03 2.028374E-04 741 189 1.162553E-01 7.498195E-03 2.076719E-04 741 190 1.168278E-01 7.737144E-03 2.031108E-04 741 191 1.174002E-01 7.510448E-03 2.050491E-04 741 192 1.179727E-01 6.637186E-03 1.962406E-04 741 193 1.185451E-01 6.899592E-03 2.045752E-04 741 194 1.191175E-01 6.784011E-03 2.006983E-04 741 195 1.196900E-01 6.771807E-03 2.042698E-04 741 196 1.202624E-01 6.727697E-03 2.014965E-04 741 197 1.208348E-01 6.224681E-03 2.022760E-04 741 198 1.214072E-01 6.115190E-03 2.005085E-04 741 199 1.219796E-01 6.274261E-03 2.006426E-04 741 200 1.225521E-01 6.090515E-03 2.026191E-04 741 201 1.231245E-01 5.876445E-03 2.025462E-04 741 202 1.236969E-01 5.721392E-03 2.027796E-04 741 203 1.242693E-01 5.637336E-03 1.970755E-04 741 204 1.248417E-01 5.550468E-03 2.025293E-04 741 205 1.254141E-01 5.403676E-03 1.956287E-04 741 206 1.259865E-01 4.950865E-03 2.025093E-04 741 207 1.265588E-01 4.731269E-03 1.953217E-04 741 208 1.271312E-01 5.606690E-03 1.950133E-04 741 209 1.277036E-01 4.761876E-03 1.923296E-04 741 210 1.282760E-01 5.190367E-03 1.974845E-04 741 211 1.288484E-01 4.842808E-03 1.963258E-04 741 212 1.294207E-01 4.538145E-03 1.982411E-04 741 213 1.299931E-01 4.746631E-03 1.988316E-04 741 214 1.305654E-01 4.243416E-03 1.963680E-04 741 215 1.311378E-01 4.259424E-03 1.942837E-04 741 216 1.317102E-01 4.076738E-03 1.944783E-04 741 217 1.322825E-01 3.900976E-03 1.974832E-04 741 218 1.328548E-01 3.886702E-03 1.970403E-04 741 219 1.334272E-01 3.903445E-03 1.990780E-04 741 220 1.339995E-01 3.786003E-03 1.840697E-04 741 221 1.345718E-01 4.079460E-03 1.757923E-04 741 222 1.351442E-01 3.887601E-03 1.786829E-04 741 223 1.357165E-01 3.820164E-03 1.841214E-04 741 224 1.362888E-01 3.756021E-03 1.804225E-04 741 225 1.368611E-01 3.698025E-03 1.844509E-04 741 226 1.374334E-01 3.590798E-03 1.871446E-04 741 227 1.380058E-01 3.366874E-03 1.841940E-04 741 228 1.385781E-01 3.312592E-03 1.867857E-04 741 229 1.391504E-01 3.537310E-03 1.870633E-04 741 230 1.397227E-01 3.709435E-03 1.908330E-04 741 231 1.402949E-01 3.067874E-03 1.842235E-04 741 232 1.408672E-01 3.293983E-03 1.873566E-04 741 233 1.414395E-01 3.116940E-03 1.937607E-04 741 234 1.420118E-01 3.079812E-03 1.836848E-04 741 235 1.425841E-01 2.815725E-03 1.881943E-04 741 236 1.431563E-01 2.873516E-03 1.842925E-04 741 237 1.437286E-01 2.976086E-03 1.880290E-04 741 238 1.443009E-01 2.979694E-03 1.853009E-04 741 239 1.448731E-01 2.491377E-03 1.795399E-04 741 240 1.454454E-01 2.466789E-03 1.871826E-04 741 241 1.460176E-01 2.637421E-03 1.889839E-04 741 242 1.465899E-01 2.740487E-03 1.927347E-04 741 243 1.471621E-01 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