{ "code": "SASDC73", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.", "publication": { "title": "Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.", "author_list": "Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH", "journal": "Structure", "doi": "10.1016/j.str.2015.02.012", "pmid": "25817386", "published_date": "2015 May 5" }, "status": "released", "submitted_date": "2017-05-18", "released_date": "2017-05-05" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDC73.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC73.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC73_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC73_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC73_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC73_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDC73.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus3 X 2M", "resolution": 172.0 }, "name": "Advanced Light Source (ALS)", "city": "Berkeley, CA", "country": "USA", "beamline_name": "12.3.1 (SIBYLS)", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Serine/threonine-protein kinase ATG1", "short_name": "Atg1", "sequence": "DSNITPAVESLAAKAFVMYSFAEMKFSQILPTPPSSTDYDPLSDKRLSNGSCAIEDEEDLDQGRPPSNQ\r\nTLTSATTKISSATNVDTQIPAPELKKLCTESLLLYLKALTILAASMKLTSKWWYENESKNCTLKLNILVQ\r\nWIRDRFNECLDKAEFLRLKLHAINTSPNSQWSDDDPVIFVEKLIYDRALDISRNAARMEMESGNYNTCEL\r\nAYATSLWMLEILLDENFQFNEVYDDEYASNITSLDESDKEMIKKYISSIANRLKALKSKMV", "organism": "Kluyveromyces lactis", "uniprot_code": "Q6CSX2", "uniprot_range_first": 562, "uniprot_range_last": 831, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MSSESHDKVVAKAIRLPTENYSVEKEIGKGSFAVVYKGLSLRDGRNIAIKAVSRSKLKNK\nKLLENLEVEIAILKKIKHPHIVGLIDCERTSSDFYLIMEYCALGDLTFFIKKRKNLVLKH\nPLIKTVFEHYPPPSTEHNGLNRVLVVNYLQQLSSALKFLRSKNLVHRDIKPQNLLLCTPL\nLDYNDPKTFHELGFVGIYNLPILKIADFGFARFLPNTSLAETLCGSPLYMAPEILNYQKY\nNAKADLWSVGTVLYEMCCGRPPFKASNHLELFQKIKKANDEITVPSNCYIEPKLFNLIRG\nLLTFDPDSRMGFTDFFNNEVVTEDLTRYEQSYEPDLESKSKDVAESNMFVSEYLVKPLKQ\nQESAHIPPTQTDENTSVQTGVRRTSGKERLATNHPPHQQIHPEDNSQNPEQSYQSASQKR\nLKSSYNDLILEKEYVVVEKKTVEVNSLADDFANNGPITNNQGAQVIKPLRYRTSSSSDAS\nGGRRASLVERRLSISSLSPSNALSKALGLASVRLFGYQHNTKATSSPPQQTLLNPQIFQE\nLTENAVLRADHKLNPFSEQMLDSNITPAVESLAAKAFVMYSFAEMKFSQILPTPPSSTDY\nDPLSDKRLSNGSCAIEDEEDLDQGRPPSNQTLTSATTKISSATNVDTQIPAPELKKLCTE\nSLLLYLKALTILAASMKLTSKWWYENESKNCTLKLNILVQWIRDRFNECLDKAEFLRLKL\nHAINTSPNSQWSDDDPVIFVEKLIYDRALDISRNAARMEMESGNYNTCELAYATSLWMLE\nILLDENFQFNEVYDDEYASNITSLDESDKEMIKKYISSIANRLKALKSKMV", "mw": 30.664, "total_mw": 61.328, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Autophagy-related protein 13", "short_name": "Atg13", "sequence": "FDRTAKPRKSTENPPEDLLEF\r\nVKLLEDKKELNMKPNTILPQQDISNSLMRFQSMKSNNDALSDNLSMSMSIDQPNV", "organism": "Kluyveromyces lactis", "uniprot_code": "Q6CWK2", "uniprot_range_first": 400, "uniprot_range_last": 475, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MSEGEVQVIELINNFFLKSALLLEQSKVAHNFDTEEALRDGNHLFNIETRGDPLLEAQIQ\nPWITFDGVKTMPPLVIETYLDLRALQPNHMVYLHDADGNPWMVCKGGKKTEIVLERWLVE\nLDKQTIDDSIDSNDPENLHKQLVLLFRYLYTLTQLLPANDIITKPHNSQQPALINVQTRL\nLDGSKPILSKGRVGLSKPIIASYSNTMNETNIASHLEQRKITPIKTTFGSLRITVSYRKD\nVDFYVIDSDDLQKRYMTPSLSNETTTVPDRRASSNCSRSMSVSPKTNTINATLFPLEGSS\nARRQSISSKLQPFKVGSVGSGSFVQSGSAQSTTSLVPSLSRNVSSSSVVAALKVQRGSAG\nSTIVNGDVPPELSSVGSGSKYSSSFGRIRRHSSIRRSESFDRTAKPRKSTENPPEDLLEF\nVKLLEDKKELNMKPNTILPQQDISNSLMRFQSMKSNNDALSDNLSMSMSIDQPNVRFGSN\nSHSPIPSFSPNYGSIPSRLSQGSRNNSNAELITSRKSSLDRHKHNLLSRTGSNVDINRRG\nSVGTMETTNEDSKEDEQSHMKGMHFSNEATINKDDDEDEMLMKRSFNAGTSTTEQVMGSP\nRSIRSISVSSYPRNQLPLKHLNLSHPTTSATTTHAKFHKSEMSPDPLHTEGAQPHTSSQH\nHNSSQKNDEDDDLLFVMSDMNLTN", "mw": 8.726, "total_mw": 17.452, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Autophagy-related protein 17", "short_name": "Atg17", "sequence": "MSDAATTIPSDEIDKLWNKARNQLVEAQVECEESLKILSKVRTEMDSSQKSRFKLKFILNCLVNQVEFFK\r\nNIMLEKCISTELIDNEWSKLVLVEIVNDVSYWQNEITTKMKILQGTKYDLTDDHSSLSDFICMDHVDILQ\r\nQKIDEIPIIKQQVTNIRQHYKSIKDRIENQLVAVKLKKLRTYFDSHFSRDSKNNLFKLLESDYVTELNEF\r\nENELADFLRSITDHFDKCTILKEQQIPLPDLKELFQIVKKDDTQLENIRELVFETGLEVKAFSKKVNETI\r\nSEIMDKIGGFHLLASKIVTELEKCEEYLSIFQKIANLVFVYKESCIKKIEQVQQLCEFYDKFKLGYKNLL\r\nRERDRRKATALQMEKILKECQEKLQALSDADLDQRQQFLLENGDYLPENIWPGYIDDMESMYSFEYSIHN\r\nVPN", "organism": "Kluyveromyces lactis", "uniprot_code": "Q6CS99", "uniprot_range_first": 1, "uniprot_range_last": 423, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MSDAATTIPSDEIDKLWNKARNQLVEAQVECEESLKILSKVRTEMDSSQKSRFKLKFILN\nCLVNQVEFFKNIMLEKCISTELIDNEWSKLVLVEIVNDVSYWQNEITTKMKILQGTKYDL\nTDDHSSLSDFICMDHVDILQQKIDEIPIIKQQVTNIRQHYKSIKDRIENQLVAVKLKKLR\nTYFDSHFSRDSKNNLFKLLESDYVTELNEFENELADFLRSITDHFDKCTILKEQQIPLPD\nLKELFQIVKKDDTQLENIRELVFETGLEVKAFSKKVNETISEIMDKIGGFHLLASKIVTE\nLEKCEEYLSIFQKIANLVFVYKESCIKKIEQVQQLCEFYDKFKLGYKNLLRERDRRKATA\nLQMEKILKECQEKLQALSDADLDQRQQFLLENGDYLPENIWPGYIDDMESMYSFEYSIHN\nVPN", "mw": 49.864, "total_mw": 99.728, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Autophagy-related protein 29", "short_name": null, "sequence": "MNNDNTKVYVRVAGKRPENFVETKPFIWDSRRDKILWTKISKIDSLEDMDWQELGADLGAPEPFLKKRSY\r\nTLFQNQLKVLSNQID", "organism": "Kluyveromyces lactis", "uniprot_code": "Q6CTU8", "uniprot_range_first": 1, "uniprot_range_last": 85, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MNNDNTKVYVRVAGKRPENFVETKPFIWDSRRDKILWTKISKIDSLEDMDWQELGADLGA\nPEPFLKKRSYTLFQNQLKVLSNQIDVTSNTRSSSESRNSVDNNILQNLQASRIMNHKLDK\nTDNLANNQESSSELSNLSVSKSALEDALMDCLQL", "mw": 10.031, "total_mw": 20.062, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "KLLA0A10637p", "short_name": "Atg31", "sequence": "MESPVLLVKNANAATNASNLGLLGQKQNSYTVDENTWFLNNISFIFEDDDPSQHDEDQLDYENVIIIDSE\r\nPSGTVNNVEMLSERWQLLAFDQNPRDNCISLTVMNELSADLGQNTDISGLDNLSKLYHERNAQIRKLLDS\r\nIAP", "organism": "Kluyveromyces lactis", "uniprot_code": "Q6CX74", "uniprot_range_first": 1, "uniprot_range_last": 143, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MESPVLLVKNANAATNASNLGLLGQKQNSYTVDENTWFLNNISFIFEDDDPSQHDEDQLD\nYENVIIIDSEPSGTVNNVEMLSERWQLLAFDQNPRDNCISLTVMNELSADLGQNTDISGL\nDNLSKLYHERNAQIRKLLDSIAP", "mw": 16.071, "total_mw": 32.142, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris, 200 mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Atg1-Atg13-Atg17-Atg31-Atg29 Minipentamer Complex", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Hanover, NH, USA", "full_name": "Dartmouth College", "webpage": "http://home.dartmouth.edu/" } ], "contributor_name": "Michael", "contributor_surname": "Ragusa", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2013-12-20", "storage_temperature": 10.0, "cell_temperature": 22.0, "exposure_time": 0.6, "number_of_frames": null, "wavelength": null, "sample_detector_distance": null, "concentration_min": 0.7, "concentration_max": 2.6, "sample_volume": null, "flow_rate": null, "s_min": 0.109, "s_max": 2.719, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": null, "experimental_mw": 234.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 768.6, "pddf_i0_error": 14.0, "guinier_i0": 967.1, "guinier_i0_error": 618.0, "pddf_rg": 9.97, "pddf_rg_error": 0.1, "guinier_rg": 10.29, "guinier_rg_error": 5.8, "pddf_dmax": 34.0, "pddf_dmax_error": null, "porod_volume": 1000.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 6, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDC73_datloglog_img.png", "symmetry": null, "last_modified": "2020-11-24T17:34:01.079622+01:00", "bragg_peak": [] }