{ "code": "SASDC75", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Structural and functional studies of the Leishmania braziliensis SGT co-chaperone indicate that it shares structural features with HIP and can interact with both Hsp90 and Hsp70 with similar affinities.", "publication": { "title": "Structural and functional studies of the Leishmania braziliensis SGT co-chaperone indicate that it shares structural features with HIP and can interact with both Hsp90 and Hsp70 with similar affinities.", "author_list": "Coto ALS, Seraphim TV, Batista FAH, Dores-Silva PR, Barranco ABF, Teixeira FR, Gava LM, Borges JC", "journal": "Int J Biol Macromol", "doi": "10.1016/j.ijbiomac.2018.06.123", "pmid": "29959008", "published_date": "2018 Oct 15" }, "status": "released", "submitted_date": "2017-06-17", "released_date": "2018-07-03" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDC75.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC75.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC75_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC75_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC75_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC75_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDC75.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "MAR 165 CCD", "resolution": null }, "name": "Brazilian Synchrotron Light Laboratory", "city": "Campinas", "country": "Brazil", "beamline_name": "SAXS2 Beamline", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "SGT protein", "short_name": "LbSGT", "sequence": "GSHMEEKDQPITDDHRKLVFSFLRMMRRSKVENPERTEAVAQMLGEEFGVDPAGSGGLHDTEV\r\nDVLEAFKTALREHERRSGAEQDEKFMSFVELLEKKGYFKGVEKGSDEYAQRLEKARDKFN\r\nQRNNPYEGLTAEQIKNKGNELMSQAKYKEAIAYYTKAIELESDNAVFFANRAAAHTHLKD\r\nYSNAIIDCERAIVINPEYSKSYSRLGTALFYQENYARAVEAFTKACDLDPDNDTYKEDLK\r\nRAEEKAKMTGLTTGGGMGGFPGMGGFPGMGGMPDMSQFASMMNNPQFMETAQRMMQNPEF\r\nSNMVANMAGKFSQGGMDPAELNRLGADMGMRNVDEEGNVVTPFGKVNRAAIEKLQEEEVR\r\nKNPKLAGIMADVQANGYGAFQKYLGDPDVMSLMMKFQSLMFTNPNSSA", "organism": "Leishmania braziliensis", "uniprot_code": "A4HIK6", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MEEKDQPITDDHRKLVFSFLRMMRRSKVENPERTEAVAQMLGEEFGVDPAGSGGLHDTEV\nDVLEAFKTALREHERRSGAEQDEKFMSFVELLEKKGYFKGVEKGSDEYAQRLEKARDKFN\nQRNNPYEGLTAEQIKNKGNELMSQAKYKEAIAYYTKAIELESDNAVFFANRAAAHTHLKD\nYSNAIIDCERAIVINPEYSKSYSRLGTALFYQENYARAVEAFTKACDLDPDNDTYKEDLK\nRAEEKAKMTGLTTGGGMGGFPGMGGFPGMGGMPDMSQFASMMNNPQFMETAQRMMQNPEF\nSNMVANMAGKFSQGGMDPAELNRLGADMGMRNVDEEGNVVTPFGKVNRAAIEKLQEEEVR\nKNPKLAGIMADVQANGYGAFQKYLGDPDVMSLMMKFQSLMFTNPNSSA", "mw": 45.935, "total_mw": 91.87, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Potassium Phosphate, 100 mM KCl, 10 mM EDTA, 1 mM B-mercaptoethanol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Leishmania braziliensis SGT co-chaperone", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "São Carlos - SP - Brasil", "full_name": "Instituto de Química de São Carlos", "webpage": "" } ], "contributor_name": "Júlio", "contributor_surname": "Borges", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2014-05-14", "storage_temperature": 4.0, "cell_temperature": 20.0, "exposure_time": 300.0, "number_of_frames": 3, "wavelength": 0.1488, "sample_detector_distance": 1.0, "concentration_min": 1.5, "concentration_max": 6.0, "sample_volume": null, "flow_rate": null, "s_min": 0.154, "s_max": 3.329, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDC75_fit1_model1_img.png", "software": "DAMFILT", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "4.1", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDC75_fit1_model1.pdb", "model_mw": 71.6, "bead_radius": null, "log": null, "symmetry": "P2", "comment": "", "user": 161 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC75_fit1_fit_img.png", "software": "DAMMIN", "chi_square_value": 1.476, "p_value": 0.203609, "fit_residual_plot": "SASDC75_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDC75_fit1.fir", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/SGT_1.log", "software_version": "5.3", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "4.6", "i0_calibration_standard": null, "description": null, "experiment_description": "SAXS experiments were performed at the D02A-SAXS2 beamline at the Brazilian Synchrotron Light Laboratory (LNLS, Campinas, São Paulo, Brazil). The X-ray scattering data (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle; λ = 0.1488 nm) were acquired using a two-dimensional position-sensitive MAR-CCD detector. Measurements were performed using a monochromatic X-ray beam and a sample-to-detector distance of ~1,000 mm, corresponding to the scattering vector range of 0.015 < q < 0.35 Å-1. LbSGT samples were prepared at 1.5 mg/mL, 3.0 mg/mL, 4.5 mg/mL and 6.0 mg/mL. Successive frames (20, 60 and 300 sec) were collected to inspect for X-ray damage. Aggregation or inter-particle interference were not observed. The model depicts the averaged spatial representation of the protein (DAMFILT occupancy and volume-corrected bead model).", "tags": [], "intensity_unit": null, "experimental_mw": 91.0, "experimental_mw_error": null, "guinier_i0_mw": 102.0, "guinier_i0_mw_error": 4.0, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 0.06631, "pddf_i0_error": 0.00296, "guinier_i0": 0.0659072, "guinier_i0_error": 0.00039, "pddf_rg": 4.66, "pddf_rg_error": 0.3, "guinier_rg": 4.48, "guinier_rg_error": 0.4, "pddf_dmax": 17.0, "pddf_dmax_error": 5.0, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 2, "guinier_point_last": 11, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDC75_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }