data_SASDF78_MAIN # _sas_result.id 1478 _sas_result.SASBDB_code SASDF78 _sas_result.experimental_MW 33.800 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 39.000 _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 7760.3000000000 _sas_result.I0_from_Guinier_error 34.0000000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 4.230 _sas_result.Rg_from_Guinier_error 0.500 _sas_result.D_max 20.00 _sas_result.Porod_volume 63.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 54 _sas_result.Guinier_point_max 136 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments . # _sas_scan.title "Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl" _sas_scan.detc_id 4 _sas_scan.id 1478 _sas_scan.measurement_date 2016-10-03 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 20.0 _sas_scan.exposure_time 0.050 _sas_scan.number_of_frames 20 _sas_scan.unit 1/nm _sas_scan.qmin 0.0134 _sas_scan.qmax 3.7925 _sas_scan.sample_id 1439 _sas_scan.result_id 1478 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.00000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1439 _sas_sample.name "Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 3.60 _sas_sample.concentration_method . _sas_sample.entity_id 1736 # _sas_buffer.id 899 _sas_buffer.name "20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 6.600 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1439 # _entity.id 1736 _entity.type protein _entity.pdbx_description "DNA-directed RNA polymerase subunit delta" _entity.formula_weight 20.399 _entity.pdbx_number_of_molecules 1 # _entity_name_com.entity_id 1736 _entity_name_com.name RpoE # _entity_poly.entity_id 1736 _entity_poly.pdbx_seq_one_letter_code MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK # _entity_src_gen.entity_id 1736 _entity_src_gen.gene_src_common_name "Bacillus subtilis (strain 168)" # _struct_ref.id 1 _struct_ref.entity_id 1736 _struct_ref.db_name UniProt _struct_ref.db_code P12464 _struct_ref.details http://www.uniprot.org/uniprot/P12464 # _citation.id 495 _citation.title "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase." _citation.year "2019 Sep 24" _citation.pdbx_database_id_DOI 10.1021/jacs.9b07837 _citation.pdbx_database_id_PubMed 31550880 # loop_ _citation_author.citation_id _citation_author.ordinal _citation_author.name 495 1 "Kuban V" 495 2 "Srb P" 495 3 "Stegnerova H" 495 4 "Padrta P" 495 5 "Zachrdla M" 495 6 "Jasenakova Z" 495 7 "Šanderová H" 495 8 "Vítovská D" 495 9 "Krasny L" 495 10 "Koval T" 495 11 "Dohnalek J" 495 12 "Ziemska-Legi Cka J" 495 13 "Grynberg M" 495 14 "Jarnot P" 495 15 "Gruca A" 495 16 "Jensen MR" 495 17 "Blackledge M" # _pdbx_contact_author.id 83 _pdbx_contact_author.name_first Vojtěch _pdbx_contact_author.name_last Kubáň _pdbx_contact_author.role . _pdbx_contact_author.address_1 MUNI _pdbx_contact_author.address_2 "Masaryk University; Research group Structure and Dynamics of Proteins." _pdbx_contact_author.address_3 "Brno, Czech Republic" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 1.337960E-02 3.260700E+04 8.509720E+03 1478 2 1.546980E-02 3.144700E+04 5.446656E+03 1478 3 1.756010E-02 2.183340E+04 3.401045E+03 1478 4 1.965030E-02 1.801820E+04 2.306816E+03 1478 5 2.174050E-02 1.707700E+04 1.889153E+03 1478 6 2.383070E-02 1.511050E+04 1.481779E+03 1478 7 2.592090E-02 1.360990E+04 1.322811E+03 1478 8 2.801120E-02 1.156780E+04 1.024818E+03 1478 9 3.010140E-02 1.132840E+04 8.935679E+02 1478 10 3.219160E-02 1.108227E+04 7.781610E+02 1478 11 3.428180E-02 9.581890E+03 6.569983E+02 1478 12 3.637210E-02 9.396650E+03 6.070282E+02 1478 13 3.846230E-02 1.016634E+04 5.086872E+02 1478 14 4.055250E-02 9.382430E+03 4.649010E+02 1478 15 4.264270E-02 9.437240E+03 4.266855E+02 1478 16 4.473290E-02 9.054200E+03 3.818346E+02 1478 17 4.682320E-02 8.786800E+03 3.666251E+02 1478 18 4.891340E-02 9.178410E+03 3.400098E+02 1478 19 5.100360E-02 8.397540E+03 3.389078E+02 1478 20 5.309380E-02 9.181400E+03 3.155249E+02 1478 21 5.518410E-02 8.945420E+03 2.955606E+02 1478 22 5.727430E-02 8.344220E+03 2.913509E+02 1478 23 5.936450E-02 8.754190E+03 2.709640E+02 1478 24 6.145470E-02 8.494560E+03 2.774429E+02 1478 25 6.354490E-02 8.324760E+03 2.461685E+02 1478 26 6.563520E-02 8.325649E+03 2.463351E+02 1478 27 6.772540E-02 7.958462E+03 2.389023E+02 1478 28 6.981560E-02 7.898192E+03 2.327778E+02 1478 29 7.190580E-02 7.995256E+03 2.184255E+02 1478 30 7.399610E-02 8.061988E+03 2.131415E+02 1478 31 7.608630E-02 8.083338E+03 2.152178E+02 1478 32 7.817650E-02 7.827777E+03 2.068244E+02 1478 33 8.026670E-02 7.783421E+03 2.034136E+02 1478 34 8.235690E-02 7.982937E+03 1.960168E+02 1478 35 8.444720E-02 8.079041E+03 1.921910E+02 1478 36 8.653740E-02 7.802082E+03 1.915490E+02 1478 37 8.862760E-02 7.494336E+03 1.815090E+02 1478 38 9.071780E-02 7.758499E+03 1.817050E+02 1478 39 9.280810E-02 7.649543E+03 1.757345E+02 1478 40 9.489830E-02 7.763267E+03 1.800673E+02 1478 41 9.698850E-02 7.864957E+03 1.787656E+02 1478 42 9.907870E-02 7.567626E+03 1.657191E+02 1478 43 1.011690E-01 7.570096E+03 1.694484E+02 1478 44 1.032590E-01 7.553430E+03 1.638270E+02 1478 45 1.053490E-01 7.436329E+03 1.658427E+02 1478 46 1.074400E-01 7.597327E+03 1.598438E+02 1478 47 1.095300E-01 7.377398E+03 1.567830E+02 1478 48 1.116200E-01 7.334607E+03 1.627332E+02 1478 49 1.137100E-01 7.389799E+03 1.546042E+02 1478 50 1.158000E-01 7.441193E+03 1.532145E+02 1478 51 1.178910E-01 7.209858E+03 1.501682E+02 1478 52 1.199810E-01 7.221907E+03 1.483184E+02 1478 53 1.220710E-01 7.185356E+03 1.518881E+02 1478 54 1.241610E-01 7.328812E+03 1.425186E+02 1478 55 1.262520E-01 7.149339E+03 1.443308E+02 1478 56 1.283420E-01 7.116789E+03 1.428649E+02 1478 57 1.304320E-01 6.978436E+03 1.399468E+02 1478 58 1.325220E-01 6.915869E+03 1.408963E+02 1478 59 1.346120E-01 7.245198E+03 1.388053E+02 1478 60 1.367030E-01 7.155866E+03 1.382413E+02 1478 61 1.387930E-01 6.984039E+03 1.331758E+02 1478 62 1.408830E-01 6.910613E+03 1.356651E+02 1478 63 1.429730E-01 6.820240E+03 1.299939E+02 1478 64 1.450640E-01 7.111868E+03 1.330840E+02 1478 65 1.471540E-01 6.821582E+03 1.329536E+02 1478 66 1.492440E-01 6.797573E+03 1.287432E+02 1478 67 1.513340E-01 6.818675E+03 1.276650E+02 1478 68 1.534240E-01 6.765032E+03 1.250972E+02 1478 69 1.555150E-01 6.796469E+03 1.261247E+02 1478 70 1.576050E-01 6.795550E+03 1.271694E+02 1478 71 1.596950E-01 6.689631E+03 1.221115E+02 1478 72 1.617850E-01 6.555604E+03 1.222072E+02 1478 73 1.638760E-01 6.573617E+03 1.197386E+02 1478 74 1.659660E-01 6.512508E+03 1.216496E+02 1478 75 1.680560E-01 6.354546E+03 1.188121E+02 1478 76 1.701460E-01 6.617738E+03 1.177512E+02 1478 77 1.722360E-01 6.506982E+03 1.199973E+02 1478 78 1.743270E-01 6.386333E+03 1.154785E+02 1478 79 1.764170E-01 6.492905E+03 1.162110E+02 1478 80 1.785070E-01 6.298170E+03 1.119109E+02 1478 81 1.805970E-01 6.234349E+03 1.137008E+02 1478 82 1.826880E-01 6.389674E+03 1.154735E+02 1478 83 1.847780E-01 6.433693E+03 1.126981E+02 1478 84 1.868680E-01 6.254102E+03 1.111540E+02 1478 85 1.889580E-01 6.077707E+03 1.086030E+02 1478 86 1.910480E-01 6.167100E+03 1.095993E+02 1478 87 1.931390E-01 6.181298E+03 1.106123E+02 1478 88 1.952290E-01 6.272126E+03 1.074833E+02 1478 89 1.973190E-01 6.108054E+03 1.092407E+02 1478 90 1.994090E-01 6.364062E+03 1.064778E+02 1478 91 2.015000E-01 6.039546E+03 1.071137E+02 1478 92 2.035900E-01 5.966579E+03 1.045280E+02 1478 93 2.056800E-01 5.906331E+03 1.025315E+02 1478 94 2.077700E-01 5.921807E+03 1.068273E+02 1478 95 2.098600E-01 5.941872E+03 1.016453E+02 1478 96 2.119510E-01 5.931410E+03 1.041095E+02 1478 97 2.140410E-01 5.932736E+03 1.005008E+02 1478 98 2.161310E-01 5.892510E+03 1.001176E+02 1478 99 2.182210E-01 5.763709E+03 1.025603E+02 1478 100 2.203120E-01 5.641998E+03 9.890771E+01 1478 101 2.224020E-01 5.642498E+03 9.814932E+01 1478 102 2.244920E-01 5.788686E+03 9.948918E+01 1478 103 2.265820E-01 5.549523E+03 9.727616E+01 1478 104 2.286720E-01 5.656820E+03 9.742695E+01 1478 105 2.307630E-01 5.502675E+03 9.549144E+01 1478 106 2.328530E-01 5.480732E+03 9.607942E+01 1478 107 2.349430E-01 5.411153E+03 9.493204E+01 1478 108 2.370330E-01 5.594634E+03 9.632784E+01 1478 109 2.391240E-01 5.442215E+03 9.451488E+01 1478 110 2.412140E-01 5.346902E+03 9.223684E+01 1478 111 2.433040E-01 5.596876E+03 9.475253E+01 1478 112 2.453940E-01 5.244604E+03 9.198317E+01 1478 113 2.474840E-01 5.422736E+03 9.396738E+01 1478 114 2.495750E-01 5.443436E+03 9.079349E+01 1478 115 2.516650E-01 5.309631E+03 9.181484E+01 1478 116 2.537550E-01 5.380498E+03 9.099881E+01 1478 117 2.558450E-01 5.212483E+03 9.052009E+01 1478 118 2.579360E-01 5.249540E+03 8.940458E+01 1478 119 2.600260E-01 5.256017E+03 8.899493E+01 1478 120 2.621160E-01 5.173589E+03 8.908211E+01 1478 121 2.642060E-01 4.979044E+03 8.769971E+01 1478 122 2.662960E-01 5.126858E+03 8.713740E+01 1478 123 2.683870E-01 5.099404E+03 8.726933E+01 1478 124 2.704770E-01 5.049658E+03 8.552261E+01 1478 125 2.725670E-01 4.923373E+03 8.757656E+01 1478 126 2.746570E-01 4.884311E+03 8.502625E+01 1478 127 2.767480E-01 4.846678E+03 8.388942E+01 1478 128 2.788380E-01 5.005464E+03 8.557076E+01 1478 129 2.809280E-01 4.976560E+03 8.441358E+01 1478 130 2.830180E-01 4.866376E+03 8.458481E+01 1478 131 2.851080E-01 4.798676E+03 8.225991E+01 1478 132 2.871990E-01 4.827536E+03 8.436102E+01 1478 133 2.892890E-01 4.698726E+03 8.193660E+01 1478 134 2.913790E-01 4.672534E+03 8.165381E+01 1478 135 2.934690E-01 4.724036E+03 8.157993E+01 1478 136 2.955600E-01 4.654390E+03 7.958913E+01 1478 137 2.976500E-01 4.801337E+03 8.309037E+01 1478 138 2.997400E-01 4.568476E+03 8.025282E+01 1478 139 3.018300E-01 4.555364E+03 7.948478E+01 1478 140 3.039200E-01 4.551335E+03 8.021557E+01 1478 141 3.060110E-01 4.495341E+03 7.792753E+01 1478 142 3.081010E-01 4.592349E+03 8.095393E+01 1478 143 3.101910E-01 4.468942E+03 7.760632E+01 1478 144 3.122810E-01 4.384079E+03 7.761536E+01 1478 145 3.143720E-01 4.461985E+03 7.841895E+01 1478 146 3.164620E-01 4.424214E+03 7.745664E+01 1478 147 3.185520E-01 4.429422E+03 7.767281E+01 1478 148 3.206420E-01 4.151348E+03 7.418661E+01 1478 149 3.227320E-01 4.480866E+03 7.735957E+01 1478 150 3.248230E-01 4.476105E+03 7.693901E+01 1478 151 3.269130E-01 4.445251E+03 7.652502E+01 1478 152 3.290030E-01 4.650375E+03 7.870340E+01 1478 153 3.310930E-01 4.153977E+03 7.296261E+01 1478 154 3.331840E-01 4.312524E+03 7.597984E+01 1478 155 3.352740E-01 4.116647E+03 7.425942E+01 1478 156 3.373640E-01 4.092030E+03 7.319130E+01 1478 157 3.394540E-01 4.269423E+03 7.435378E+01 1478 158 3.415440E-01 4.161560E+03 7.294494E+01 1478 159 3.436350E-01 4.120018E+03 7.356635E+01 1478 160 3.457250E-01 3.948485E+03 7.133725E+01 1478 161 3.478150E-01 3.972628E+03 7.230637E+01 1478 162 3.499050E-01 4.074777E+03 7.537065E+01 1478 163 3.519960E-01 4.051835E+03 7.732741E+01 1478 164 3.540860E-01 3.854582E+03 7.866544E+01 1478 165 3.561760E-01 3.978527E+03 7.672158E+01 1478 166 3.582660E-01 4.012947E+03 7.988499E+01 1478 167 3.603560E-01 4.003707E+03 8.027359E+01 1478 168 3.624470E-01 3.897586E+03 8.152957E+01 1478 169 3.645370E-01 3.864113E+03 8.108175E+01 1478 170 3.666270E-01 3.717619E+03 8.090100E+01 1478 171 3.687170E-01 3.708428E+03 8.272153E+01 1478 172 3.708080E-01 3.714928E+03 8.172954E+01 1478 173 3.728980E-01 3.818569E+03 8.110916E+01 1478 174 3.749880E-01 3.785423E+03 8.117294E+01 1478 175 3.770780E-01 3.641078E+03 8.184375E+01 1478 176 3.791680E-01 3.665908E+03 8.212994E+01 1478 177 3.812590E-01 3.712401E+03 7.945008E+01 1478 178 3.833490E-01 3.663560E+03 8.196360E+01 1478 179 3.854390E-01 3.743800E+03 8.152034E+01 1478 180 3.875290E-01 3.517453E+03 8.121587E+01 1478 181 3.896200E-01 3.602694E+03 8.267283E+01 1478 182 3.917100E-01 3.483513E+03 7.887785E+01 1478 183 3.938000E-01 3.577218E+03 7.988661E+01 1478 184 3.958900E-01 3.409311E+03 7.495730E+01 1478 185 3.979800E-01 3.534943E+03 7.562322E+01 1478 186 4.000710E-01 3.240431E+03 7.370603E+01 1478 187 4.021610E-01 3.311050E+03 7.216032E+01 1478 188 4.042510E-01 3.341088E+03 7.448307E+01 1478 189 4.063410E-01 3.418586E+03 7.135910E+01 1478 190 4.084320E-01 3.425215E+03 7.227448E+01 1478 191 4.105220E-01 3.333465E+03 7.087946E+01 1478 192 4.126120E-01 3.262551E+03 6.974111E+01 1478 193 4.147020E-01 3.261919E+03 7.119397E+01 1478 194 4.167920E-01 3.255508E+03 6.860903E+01 1478 195 4.188830E-01 3.325417E+03 6.908288E+01 1478 196 4.209730E-01 3.325400E+03 6.866364E+01 1478 197 4.230630E-01 3.190784E+03 6.830549E+01 1478 198 4.251530E-01 3.086557E+03 6.770187E+01 1478 199 4.272440E-01 3.128956E+03 6.647344E+01 1478 200 4.293340E-01 3.195150E+03 6.725014E+01 1478 201 4.314240E-01 3.192253E+03 6.620680E+01 1478 202 4.335140E-01 2.970458E+03 6.611144E+01 1478 203 4.356040E-01 2.995866E+03 6.481382E+01 1478 204 4.376950E-01 3.158980E+03 6.550600E+01 1478 205 4.397850E-01 3.049821E+03 6.559096E+01 1478 206 4.418750E-01 2.882551E+03 6.323752E+01 1478 207 4.439650E-01 2.970719E+03 6.376047E+01 1478 208 4.460560E-01 2.989267E+03 6.400720E+01 1478 209 4.481460E-01 2.890054E+03 6.325483E+01 1478 210 4.502360E-01 2.995355E+03 6.493524E+01 1478 211 4.523260E-01 3.058061E+03 6.193635E+01 1478 212 4.544160E-01 2.874249E+03 6.302610E+01 1478 213 4.565070E-01 2.904726E+03 6.266968E+01 1478 214 4.585970E-01 2.855346E+03 6.203851E+01 1478 215 4.606870E-01 2.843911E+03 6.130199E+01 1478 216 4.627770E-01 2.786278E+03 6.092153E+01 1478 217 4.648680E-01 2.861478E+03 6.194319E+01 1478 218 4.669580E-01 2.878466E+03 6.098254E+01 1478 219 4.690480E-01 2.713038E+03 6.117997E+01 1478 220 4.711380E-01 2.751398E+03 5.957301E+01 1478 221 4.732280E-01 2.716023E+03 6.039697E+01 1478 222 4.753190E-01 2.770342E+03 6.004284E+01 1478 223 4.774090E-01 2.675984E+03 5.974706E+01 1478 224 4.794990E-01 2.734652E+03 5.940658E+01 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