{ "code": "SASD282", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/A", "project": { "title": "Robust structural, kinetic and biophysical characterization of wild-type human ACOD1, selected mutants and their interaction with citraconate", "publication": { "title": "Robust structural, kinetic and biophysical characterization of wild-type human ACOD1, selected mutants and their interaction with citraconate", "author_list": "Runge B, Oktay H, Fucci I, Merten E, Tarasov S, Fan L, Monteiro D", "journal": "Journal of Structural Biology: X", "doi": "10.1016/j.yjsbx.2026.100157", "pmid": null, "published_date": "2026 Aug" }, "status": "released", "submitted_date": "2026-06-24", "released_date": "2026-08-10" }, "pddf_data": null, "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASD282.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD282_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD282_kratky_img.png", "pddf_plot": null, "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD282_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASD282.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Eiger2 S", "resolution": null }, "name": "Advanced Photon Source (APS), Argonne National Laboratory", "city": "Lemont, IL", "country": "USA", "beamline_name": "12-ID-B", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "cis-aconitate decarboxylase 1", "short_name": "ACOD1", "sequence": "GGGRKSITESFATAIHGLKVGHLTDRVIQRSKRMILDTLGAGFLGTTTEVFHIASQYSKIYSSNISSTVWGQPDIRLPPTYAAFVNGVAIHSMDFDDTWHPATHPSGAVLPVLTALAEALPRSPKFSGLDLLLAFNVGIEVQGRLLHFAKEANDMPKRFHPPSVVGTLGSAAAASKFLGLSSTKCREALAIAVSHAGAPMANAATQTKPLHIGNAAKHGIE", "organism": "Homo sapiens", "uniprot_code": "A6NK06", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MMLKSITESFATAIHGLKVGHLTDRVIQRSKRMILDTLGAGFLGTTTEVFHIASQYSKIY\nSSNISSTVWGQPDIRLPPTYAAFVNGVAIHSMDFDDTWHPATHPSGAVLPVLTALAEALP\nRSPKFSGLDLLLAFNVGIEVQGRLLHFAKEANDMPKRFHPPSVVGTLGSAAAASKFLGLS\nSTKCREALAIAVSHAGAPMANAATQTKPLHIGNAAKHGIEAAFLAMLGLQGNKQVLDLEA\nGFGAFYANYSPKVLPSIASYSWLLDQQDVAFKRFPAHLSTHWVADAAASVRKHLVAERAL\nLPTDYIKRIVLRIPNVQYVNRPFPVSEHEARHSFQYVACAMLLDGGITVPSFHECQINRP\nQVRELLSKVELEYPPDNLPSFNILYCEISVTLKDGATFTDRSDTFYGHWRKPLSQEDLEE\nKFRANASKMLSWDTVESLIKIVKNLEDLEDCSVLTTLLKGPSPPEVASNSPACNNSITNL\nS", "mw": 50.596, "total_mw": 101.192, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" } ], "buffer": { "name": "20 mM HEPES , 150 mM NaCl, 0.5 mM TCEP, and 2% (v/v) glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Citraconate-bound Cis-aconitate decarboxylase (ACOD1)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": null, "full_name": "Frederick Nationla Lab for Cancer Research", "webpage": null } ], "contributor_name": "Lixin", "contributor_surname": "Fan", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2026-03-06", "storage_temperature": -20.0, "cell_temperature": 20.0, "exposure_time": 0.3, "number_of_frames": 769, "wavelength": 0.0932, "sample_detector_distance": 2.0, "concentration_min": null, "concentration_max": 6.0, "sample_volume": 100.0, "flow_rate": 0.3, "s_min": 0.05, "s_max": 5.0, "total_exposure_time": null, "seccolumn": 2 }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASD282_fit1_model1_img.png", "software": "Other", "pdb_link": [], "model_title": null, "type_of_model": "other", "software_version": "N/A", "model_data": "https://www.sasbdb.org/media/pdb_file/SASD282_fit1_model1.pdb", "model_mw": null, "bead_radius": null, "log": "https://www.sasbdb.org/media/log_files/9O5N_citrabonded.log", "symmetry": null, "comment": "Model derived from unpublished crystal structure, assigned PDB ID: 9O5N", "user": 480 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASD282_fit1_fixed_fit_img.png", "software": "CRYSOL", "chi_square_value": 0.231, "p_value": 0.0005, "fit_residual_plot": "SASD282_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASD282_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/9O5N_citrabonded.log", "software_version": "2.8.3", "description": "" } ], "estimated_volume_method": null, "pddf_software": null, "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": "1/cm", "experimental_mw": 97.2, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 0.0881514, "guinier_i0_error": null, "pddf_rg": null, "pddf_rg_error": null, "guinier_rg": 3.176, "guinier_rg_error": 0.006, "pddf_dmax": null, "pddf_dmax_error": null, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 7, "guinier_point_last": 145, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASD282_datloglog_img.png", "symmetry": null, "last_modified": "2026-08-10T15:33:56.279344+02:00", "bragg_peak": [] }