{ "code": "SASDC83", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution.", "publication": { "title": "Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution.", "author_list": "Shih O, Yeh YQ, Liao KF, Sung TC, Chiang YW, Jeng US", "journal": "Phys Chem Chem Phys", "doi": "10.1039/c6cp08820a", "pmid": "28262878", "published_date": "2017 Mar 15" }, "status": "released", "submitted_date": "2017-05-03", "released_date": "2017-05-05" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDC83.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC83.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC83_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC83_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC83_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC83_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDC83.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 1M-F", "resolution": 172.0 }, "name": "Taiwan Photon Source, NSRRC", "city": "Hsinchu", "country": "Taiwan", "beamline_name": "23A", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Apoptosis regulator BAX (Bcl-2 associated X)", "short_name": "BAX", "sequence": "MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMI\r\nAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG", "organism": "Homo sapiens", "uniprot_code": "Q07812", "uniprot_range_first": 1, "uniprot_range_last": 192, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLS\nECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKL\nVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGV\nLTASLTIWKKMG", "mw": 21.184, "total_mw": 42.368, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20mM sodium phosphate 100mM NaCl", "concentration_unit": null, "comment": "20mM sodium phosphate, 100mM NaCl", "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Dimeric apoptosis regulator BAX (Bcl-2 associated X)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Hsinchu City, Taiwan", "full_name": "National Synchrotron Radiation Research Center", "webpage": "https://www.nsrrc.org.tw/" } ], "contributor_name": "Orion", "contributor_surname": "Shih", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2015-05-28", "storage_temperature": null, "cell_temperature": 15.0, "exposure_time": 30.0, "number_of_frames": 3, "wavelength": 0.08266, "sample_detector_distance": 3.17, "concentration_min": null, "concentration_max": 2.0, "sample_volume": null, "flow_rate": null, "s_min": 0.144, "s_max": 2.991, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDC83_fit1_model1_img.png", "software": "CORAL", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDC83_fit1_model1.pdb", "model_mw": 44.72, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 216 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC83_fit1_fit_img.png", "software": "CORAL", "chi_square_value": 1.25, "p_value": 0.565228, "fit_residual_plot": "SASDC83_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDC83_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/ad28.log", "software_version": "", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of dimeric apoptosis regulator BAX (Bcl-2 associated X) in 20mM sodium phosphate, 100mM NaCl, pH 8 were collected on the 23A beam line at the Taiwan Photon Source (NSRRC, Hsinchu, Taiwan) using a Pilatus 1M-F detector at a sample-detector distance of 3.2 m and at a wavelength of λ = 0.08266 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 15°C. Three successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to obtain the SAXS profile displayed in this entry.", "tags": [], "intensity_unit": null, "experimental_mw": 39.5, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 0.01585, "pddf_i0_error": 0.000135, "guinier_i0": 0.01578, "guinier_i0_error": 0.0002, "pddf_rg": 3.01, "pddf_rg_error": 0.03, "guinier_rg": 2.97, "guinier_rg_error": 0.06, "pddf_dmax": 9.52, "pddf_dmax_error": null, "porod_volume": 85.57, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 6, "guinier_point_last": 38, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDC83_datloglog_img.png", "symmetry": null, "last_modified": "2021-12-03T19:22:36.894202+01:00", "bragg_peak": [] }