{ "code": "SASDX83", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/A", "project": { "title": "Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin", "publication": null, "status": "released", "submitted_date": "2025-04-02", "released_date": "2026-01-22" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDX83.dat", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDX83.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDX83_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDX83_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDX83.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Eiger 4M", "resolution": 75.0 }, "name": "Diamond Light Source", "city": "Didcot", "country": "UK", "beamline_name": "B21", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Histone H2A", "short_name": "H2A", "sequence": "MATPKQAVKKASKGGSSRSVKAGLIFPVGRVGTLLRRGQYARRIGASGAVYMAAVLEYLTAELLELSVKAAAQQTKKTKRLTPRTVTLAVRHDDDLGALLRNVTMSRGGVMPSLNKALAKKQKSGKHAKATPSV", "organism": "Trypanosoma brucei brucei (strain 927/4 GUTat10.1)", "uniprot_code": "Q57YA3", "uniprot_range_first": 2, "uniprot_range_last": 134, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MATPKQAVKKASKGGSSRSVKAGLIFPVGRVGTLLRRGQYARRIGASGAVYMAAVLEYLT\nAELLELSVKAAAQQTKKTKRLTPRTVTLAVRHDDDLGALLRNVTMSRGGVMPSLNKALAK\nKQKSGKHAKATPSV", "mw": 14.212, "total_mw": 28.424, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Histone H2B", "short_name": "H2B", "sequence": "ATPKSTPAKTRKEAKKTRRQRKRTWNVYVSRSLRSINSQMSMTSRTMKIVNSFVNDLFERIAAEAATIVRVNRKRTLGARELQTAVRLVLPADLAKHAMAEGTKAVSHASS", "organism": "Trypanosoma brucei brucei (strain 927/4 GUTat10.1)", "uniprot_code": "Q389T1", "uniprot_range_first": 2, "uniprot_range_last": 112, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MATPKSTPAKTRKEAKKTRRQRKRTWNVYVSRSLRSINSQMSMTSRTMKIVNSFVNDLFE\nRIAAEAATIVRVNRKRTLGARELQTAVRLVLPADLAKHAMAEGTKAVSHASS", "mw": 12.438, "total_mw": 24.876, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Histone H3, putative", "short_name": "H3", "sequence": "SRTKETARTKKTITSKKSKKASKGSDAASGVKTAQRRWRPGTVALREIRQFQRSTDLLLQKAPFQRLVREVSGAQKEGLRFQSSAILAAQEATESYIVSLLADTNRACIHSGRVTIQPKDIHLALCLRGERA", "organism": "Trypanosoma brucei brucei (strain 927/4 GUTat10.1)", "uniprot_code": "Q4GYX7", "uniprot_range_first": 2, "uniprot_range_last": 133, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MSRTKETARTKKTITSKKSKKASKGSDAASGVKTAQRRWRPGTVALREIRQFQRSTDLLL\nQKAPFQRLVREVSGAQKEGLRFQSSAILAAQEATESYIVSLLADTNRACIHSGRVTIQPK\nDIHLALCLRGERA", "mw": 14.619, "total_mw": 29.238, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Histone H4", "short_name": "H4", "sequence": "AKGKKSGEAKGSQKRQKKVLRENVRGITRGSIRRLARRGGVKRISGVIYDEVRGVLKSFVEGVVRDATAYTEYSRKKTVTAVDVVNALRKRGKILYGYA", "organism": "Trypanosoma brucei brucei (strain 927/4 GUTat10.1)", "uniprot_code": "Q57Z31", "uniprot_range_first": 2, "uniprot_range_last": 100, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MAKGKKSGEAKGSQKRQKKVLRENVRGITRGSIRRLARRGGVKRISGVIYDEVRGVLKSF\nVEGVVRDATAYTEYSRKKTVTAVDVVNALRKRGKILYGYA", "mw": 11.011, "total_mw": 22.022, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Widom 601 145 bp DNA - strand 1", "short_name": null, "sequence": "ATCGATGTATATATCTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTGAT", "organism": null, "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "other", "molecular_type": "DNA", "uniprot_sequence": "", "mw": 45.055, "total_mw": 45.055, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Widom 601 145 bp DNA - strand 2", "short_name": null, "sequence": "ATCAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACCTGTCAGATATATACATCGAT", "organism": null, "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "other", "molecular_type": "DNA", "uniprot_sequence": "", "mw": 44.543, "total_mw": 44.543, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" } ], "buffer": { "name": "20 mM HEPES, 150 mM NaCl, 1 mM DTT", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "T. brucei Canonical Nucleosome Core Particle", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [], "concentration_method": null, "concentration_unit": null, "date": "2023-10-10", "storage_temperature": 4.0, "cell_temperature": 15.0, "exposure_time": 3.0, "number_of_frames": 600, "wavelength": 0.09464, "sample_detector_distance": 3.7212, "concentration_min": null, "concentration_max": 1.1, "sample_volume": 50.0, "flow_rate": 0.08, "s_min": 0.045, "s_max": 3.4, "total_exposure_time": null, "seccolumn": 14 }, "fits": [], "estimated_volume_method": null, "pddf_software": "ScÅtter", "pddf_software_version": "IV", "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": "1/cm", "experimental_mw": 203.028, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 0.0599, "pddf_i0_error": null, "guinier_i0": 0.0602744, "guinier_i0_error": null, "pddf_rg": 4.17, "pddf_rg_error": null, "guinier_rg": 4.262, "guinier_rg_error": 0.066, "pddf_dmax": 11.75, "pddf_dmax_error": null, "porod_volume": 435.41, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 65, "guinier_point_last": 200, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDX83_datloglog_img.png", "symmetry": null, "last_modified": "2026-01-22T11:46:04.158592+01:00", "bragg_peak": [] }