{
  "code": "SASDA85",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.",
    "publication": {
      "title": "The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.",
      "author_list": "Watson AA, Mahajan P, Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED",
      "journal": "J Mol Biol",
      "doi": "10.1016/j.jmb.2012.04.031",
      "pmid": "22575888",
      "published_date": "2012 Sep 7"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDA85.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDA85.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA85_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA85_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDA85_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA85_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDA85.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Custom-built",
        "name": "Pilatus 1M-W",
        "resolution": 0.172
      },
      "name": "DORIS III, DESY",
      "city": "Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL X33",
      "beam_geometry": "0.60",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Human Chromatin Remodeler CHD4 (363-1353)",
          "short_name": "CHD4 (PP-CC-AH-D)",
          "sequence": "MGHHHHHHSSGVDLGTENLYFQSMDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHC\r\nEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLC\r\nPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELH\r\nCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLI\r\nKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEY\r\nLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREF\r\nEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACL\r\nIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKL\r\nHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHP\r\nYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDG\r\nGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRF\r\nVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYD\r\nDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQ\r\nEDLARNLGKGKRIRKQVNYNDGSQEDR",
          "organism": "Homo sapiens",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
          "mw": 117.4,
          "total_mw": 117.4,
          "number_molecules": 1,
          "complex_state": null,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50 mM HEPES 5% Glycerol 300 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": "5.0% Glycerol, 300 mM NaCl",
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "CHD4 (PP-CC-AH-D)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
            "webpage": "http://www.embl-hamburg.de/index.php"
          }
        ],
        "contributor_name": "Haydyn",
        "contributor_surname": "Mertens",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2010-11-13",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 15.0,
    "number_of_frames": 8,
    "wavelength": 0.15,
    "sample_detector_distance": 2.7,
    "concentration_min": 1.0,
    "concentration_max": 8.3,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.136,
    "s_max": 6.016,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA85_fit1_model1_img.png",
          "software": "DAMMIF",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": "",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA85_fit1_model1.pdb",
          "model_mw": 202.5,
          "bead_radius": 3.6,
          "log": "https://www.sasbdb.org/media/log_files/a08_2.log",
          "symmetry": "",
          "comment": "",
          "user": 12
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA85_fit1_fit_img.png",
      "software": null,
      "chi_square_value": 1.909924,
      "p_value": 0.0,
      "fit_residual_plot": "SASDA85_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA85_fit1.fir",
      "fit_log": null,
      "software_version": null,
      "description": null
    },
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA85_fit2_model1_img.png",
          "software": "MONSA",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": "",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA85_fit2_model1.pdb",
          "model_mw": null,
          "bead_radius": 5.0,
          "log": "https://www.sasbdb.org/media/log_files/a08mul.log",
          "symmetry": "",
          "comment": "Combined multiphase model",
          "user": 12
        },
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA85_fit2_model2_img.png",
          "software": "MONSA",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": "",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA85_fit2_model2.pdb",
          "model_mw": null,
          "bead_radius": 5.0,
          "log": null,
          "symmetry": "",
          "comment": "",
          "user": 12
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA85_fit2_fit_img.png",
      "software": null,
      "chi_square_value": 3.629025,
      "p_value": 0.0,
      "fit_residual_plot": "SASDA85_fit2_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA85_fit2.fit",
      "fit_log": null,
      "software_version": null,
      "description": null
    },
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA85_fit3_model1_img.png",
          "software": "MONSA",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": "",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA85_fit3_model1.pdb",
          "model_mw": null,
          "bead_radius": 5.0,
          "log": null,
          "symmetry": "",
          "comment": "",
          "user": 12
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA85_fit3_fit_img.png",
      "software": null,
      "chi_square_value": 2.778889,
      "p_value": 0.0,
      "fit_residual_plot": "SASDA85_fit3_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA85_fit3.fit",
      "fit_log": null,
      "software_version": null,
      "description": null
    },
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA85_fit4_model1_img.png",
          "software": "MONSA",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": "",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA85_fit4_model1.pdb",
          "model_mw": null,
          "bead_radius": 5.0,
          "log": null,
          "symmetry": "",
          "comment": "",
          "user": 12
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA85_fit4_fit_img.png",
      "software": null,
      "chi_square_value": 1.098304,
      "p_value": 0.214136,
      "fit_residual_plot": "SASDA85_fit4_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA85_fit4.fit",
      "fit_log": null,
      "software_version": null,
      "description": null
    },
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA85_fit5_model1_img.png",
          "software": "MONSA",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": "",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA85_fit5_model1.pdb",
          "model_mw": null,
          "bead_radius": 5.0,
          "log": null,
          "symmetry": "",
          "comment": "",
          "user": 12
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA85_fit5_fit_img.png",
      "software": null,
      "chi_square_value": 2.3716,
      "p_value": 0.0,
      "fit_residual_plot": "SASDA85_fit5_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA85_fit5.fit",
      "fit_log": null,
      "software_version": null,
      "description": null
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": "BSA",
  "description": "The ATPase CHD4 mediates nucleosome remodeling by the NuRD (nucleosome remodeling and deacetylase) complex. The NuRD complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The three dimensional small-angle X-ray scattering model of CHD4 helps to define its interdomain interactions, with cross linking and limited proteolysis studies used to validate the model. Functional and binding assays suggest a regulatory role for the PHD and chromo domains.",
  "experiment_description": null,
  "tags": [
    "X33"
  ],
  "intensity_unit": null,
  "experimental_mw": 119.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": 119.0,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": null,
  "pddf_i0_error": null,
  "guinier_i0": 109.38,
  "guinier_i0_error": null,
  "pddf_rg": 5.0,
  "pddf_rg_error": null,
  "guinier_rg": 4.9,
  "guinier_rg_error": null,
  "pddf_dmax": 17.4,
  "pddf_dmax_error": null,
  "porod_volume": null,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": null,
  "guinier_point_last": null,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDA85_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-05-25T11:21:52.654103+02:00",
  "bragg_peak": []
}