{ "code": "SASDC85", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Identification of two p23 co-chaperone isoforms in Leishmania braziliensis exhibiting similar structures and Hsp90 interaction properties despite divergent stabilities.", "publication": { "title": "Identification of two p23 co-chaperone isoforms in Leishmania braziliensis exhibiting similar structures and Hsp90 interaction properties despite divergent stabilities.", "author_list": "Batista FA, Almeida GS, Seraphim TV, Silva KP, Murta SM, Barbosa LR, Borges JC", "journal": "FEBS J", "doi": "10.1111/febs.13141", "pmid": "25369258", "published_date": "2015 Jan" }, "status": "released", "submitted_date": "2017-06-17", "released_date": "2017-06-21" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDC85.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC85.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC85_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC85_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC85_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC85_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDC85.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "MAR 165 CCD", "resolution": null }, "name": "Brazilian Synchrotron Light Laboratory", "city": "Campinas", "country": "Brazil", "beamline_name": "SAXS2 Beamline", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Leishmania braziliensis p23 isoform B", "short_name": "Lbp23B", "sequence": "GSHMSASGSLVPPISWAQRPEYVLVTIPLQDTTGVTVEIKDEGRELLFACCAPEGKQYACTIHFYGAISS\r\nEESQHVVRPRQIELKLRKKLTRSLEDADDEVEWPRLTKEKVKYPNITIDWSKWKDEDDEGATDDLGDFGL\r\nGGGDAMDGQYSEMLSQLMQTQGKKDAEELAGLPPGTIPEFGSAQRQEATHGASAAAGDDDSEMPPLEDDM", "organism": "Leishmania braziliensis", "uniprot_code": "A4HAD9", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MSASGSLVPPISWAQRPEYVLVTIPLQDTTGVTVEIKDEGRELLFACCAPEGKQYACTIH\nFYGAISSEESQHVVRPRQIELKLRKKLTRSLEDADDEVEWPRLTKEKVKYPNITIDWSKW\nKDEDDEGATDDLGDFGLGGGDAMDGQYSEMLSQLMQTQGKKDAEELAGLPPGTIPEFGSA\nQRQEATHGASAAAGDDDSEMPPLEDDM", "mw": 23.045, "total_mw": 23.045, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Leishmania braziliensis p23B", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "São Carlos - SP - Brasil", "full_name": "Instituto de Química de São Carlos", "webpage": "" } ], "contributor_name": "Júlio", "contributor_surname": "Borges", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2012-03-26", "storage_temperature": 4.0, "cell_temperature": 20.0, "exposure_time": 300.0, "number_of_frames": null, "wavelength": 0.1488, "sample_detector_distance": 1.0, "concentration_min": 1.0, "concentration_max": 4.0, "sample_volume": null, "flow_rate": null, "s_min": 0.162, "s_max": 3.346, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDC85_fit1_model1_img.png", "software": "DAMFILT", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "5.0", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDC85_fit1_model1.pdb", "model_mw": 34.41, "bead_radius": null, "log": null, "symmetry": "P1", "comment": "", "user": 161 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC85_fit1_fit_img.png", "software": "DAMMIN", "chi_square_value": 2.83, "p_value": 5.4e-05, "fit_residual_plot": "SASDC85_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDC85_fit1.fir", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Lbp23B5.log", "software_version": "5.3", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "4.5a", "i0_calibration_standard": null, "description": null, "experiment_description": "SAXS experiments were performed at the SAXS2 beam line in the Laboratório Nacional de Luz Síncrotron (LNLS, Campinas-SP, Brazil). The X-ray scattering data (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle; λ = 0.1488 nm) were acquired using a two-dimensional MAR-CCD detector. Measurements were performed with a monochromatic X-ray beam and a sample-to-detector distance of ~1000 mm, corresponding to a scattering vector range of 0.015 < q < 0.35 Å−1. Scattering patterns were recorded at different sample concentrations (approximately 1.0, 2.0 and 4.0 mg/mL) and several frames in order to check for protein aggregation and X-ray damage. No evidence of aggregation or inter-particle interference were observed. The model depicts the averaged spatial representation of the protein (DAMFILT occupancy and volume-corrected bead model).", "tags": [], "intensity_unit": null, "experimental_mw": 26.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 0.02014, "pddf_i0_error": 0.0004, "guinier_i0": 0.0200666, "guinier_i0_error": 0.00014, "pddf_rg": 3.17, "pddf_rg_error": 0.2, "guinier_rg": 3.03, "guinier_rg_error": 0.07, "pddf_dmax": 13.0, "pddf_dmax_error": 5.0, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 10, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDC85_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }