{ "code": "SASDE92", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties.", "publication": { "title": "A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties.", "author_list": "Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ", "journal": "Sci Rep", "doi": "10.1038/s41598-019-44385-y", "pmid": "31138855", "published_date": "2019 May 28" }, "status": "released", "submitted_date": "2018-07-30", "released_date": "2019-05-28" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDE92.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDE92.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE92_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE92_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE92_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE92_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE92.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "Diamond Light Source", "city": "Didcot", "country": "UK", "beamline_name": "B21", "beam_geometry": "1 x 5 mm", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Endoribonuclease E", "short_name": "RNase E", "sequence": "HHHHHHHHHHSSGHIEGRHMKRMLINATHAEEVRVALITGNRLYDFDLENRTREQKKSNIYKGHVTRVEPSLEAVFVEYGAGRQGFLSMREIANSYFQADPRQTSNIRELITEGTELLVQVEKEERGNKGAALSTFISLAGRYLVLMPNNPKGGGISRQISGSVREELKEILASLNVPRGMSVIVRTAGIGRTQEELQLDLQHLLDLWAQIQGTASSGPSPMLVHQEAGVVTRAIRDYLRDDVAEILIDSEQAYNEAYNFVKAVMPRQLDKLKTYTLNEPLFAHFGIESQIQTAYEREVKLPSGGSIVIDQTEALVSIDINSAKSTRGHDVEETALNTNLEAAEEIARQLRLRDIGGLVVIDFIDMTKERNQRMVEAKLREATQSDRARIQFGQLSRFGLMEMSRQRLRPSLEEATGYVCPRCHGTGMVRDLRSLSLSIMRKVEEIALRERHGEVQVEVPVEIAAFLLNEKRHSLVYLEQTSGVRVTVLPHPHLETPHYEIAYNPDGFAPSSYERTEATRSSEKELGYESSEWHLEEADHGHAHVTATASTHAAAQKKANHAT", "organism": "Acinetobacter baumannii", "uniprot_code": "A0A0B9WR03", "uniprot_range_first": 1, "uniprot_range_last": 544, "oligomerization": "tetramer", "molecular_type": "protein", "uniprot_sequence": "MKRMLINATHAEEVRVALITGNRLYDFDLENRTREQKKSNIYKGHVTRVEPSLEAVFVEY\nGAGRQGFLSMREIANSYFQADPRQTSNIRELITEGTELLVQVEKEERGNKGAALSTFISL\nAGRYLVLMPNNPKGGGISRQISGSVREELKEILASLNVPRGMSVIVRTAGIGRTQEELQL\nDLQHLLDLWAQIQGTASSGPSPMLVHQEAGVVTRAIRDYLRDDVAEILIDSEQAYNEAYN\nFVKAVMPRQLDKLKTYTLNEPLFAHFGIESQIQTAYEREVKLPSGGSIVIDQTEALVSID\nINSAKSTRGHDVEETALNTNLEAAEEIARQLRLRDIGGLVVIDFIDMTKERNQRMVEAKL\nREATQSDRARIQFGQLSRFGLMEMSRQRLRPSLEEATGYVCPRCHGTGMVRDLRSLSLSI\nMRKVEEIALRERHGEVQVEVPVEIAAFLLNEKRHSLVYLEQTSGVRVTVLPHPHLETPHY\nEIAYNPDGFAPSSYERTEATRSSEKELGYESSEWHLEEADHGHAHVTATASTHAAAQKKA\nNHATQPVAQPSAQKAASPCAWLENLFVQKQAQTVDQSRSAQNAAAAIEQMVNTGAVSRGQ\nFGQVAVPAVAEVAPVQSNNAYISQSPVKQDVREHVEKDDKSQQQRQNNKKRKHKEQREQH\nHQSHEQQHQVHEEVVQLSRQEQRELKRQQKRQQQQDQQHQNNDVQHTENAVPRRDRNNQQ\nRPNRPNRHRDPSVLNENQNTPVVIDEKQIKVDVIDAPKHDVMNTALIINVDQGQSEIVAL\nTPERRHVERVETTSTEVAQEPTPAPVVAEKAAVVETKEEAQPSQEAAQPQIKRASNDPRM\nRRRQQREAKHAKAATPSIAPSQIPTLAQHTIGSLIRHVYGEDCTVLIEQFGLVPTFNRAL\nQKFAEQYASTLVVEVTAETEEKKPVTRDAELPSHKPAEEAEPAPVLPLTPPQAPAPRVAN\nDPRERRRLAKLAAEQAFEQVKQQHSAQEEVATPAPVAEETVAAPTAETQATVEPAQQPLE\nLNQSTEVVQPEAAPVEEKATEETVAEAPAAKEPAPSKAASKAKAAAEETVAPTEATTDAE\nSEDVKADKDKPSRPRRPRGRPPKKANPVAE", "mw": 63.396, "total_mw": 253.584, "number_molecules": 4, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Ribonuclease E from Acinetobacter baumannii", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Portsmouth, UK", "full_name": "University of Portsmouth", "webpage": "https://www.port.ac.uk" } ], "contributor_name": "A", "contributor_surname": "Callaghan", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2017-02-11", "storage_temperature": 21.0, "cell_temperature": 15.0, "exposure_time": 3.0, "number_of_frames": 640, "wavelength": 0.1, "sample_detector_distance": 4.014, "concentration_min": null, "concentration_max": 5.52, "sample_volume": null, "flow_rate": null, "s_min": 0.04, "s_max": 4.42, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Ribonuclease E from Acinetobacter baumannii in 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH 8 were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.52 mg/ml was measured at 15°C. 640 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 298.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 317.0, "porod_mw_error": null, "pddf_i0": 0.01877, "pddf_i0_error": null, "guinier_i0": 0.0189, "guinier_i0_error": 6.4e-05, "pddf_rg": 5.12, "pddf_rg_error": null, "guinier_rg": 5.179, "guinier_rg_error": 0.03, "pddf_dmax": 18.26, "pddf_dmax_error": null, "porod_volume": 508.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 22, "guinier_point_last": 88, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDE92_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }