{ "code": "SASDA95", "status": "Published", "type_of_curve": "Extrapolated to infinite dilution", "angular_unit": "1/nm", "project": { "title": "The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.", "publication": { "title": "The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.", "author_list": "Watson AA, Mahajan P, Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED", "journal": "J Mol Biol", "doi": "10.1016/j.jmb.2012.04.031", "pmid": "22575888", "published_date": "2012 Sep 7" }, "status": "released", "submitted_date": null, "released_date": "2015-07-07" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDA95.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDA95.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA95_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA95_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDA95_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA95_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDA95.sascif", "experiment": { "instrument": { "detector": { "type": "Custom-built", "name": "Pilatus 1M-W", "resolution": 0.172 }, "name": "DORIS III, DESY", "city": "Hamburg", "country": "Germany", "beamline_name": "EMBL X33", "beam_geometry": "0.60", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Human Chromatin Remodeler CHD4 (494-1353)", "short_name": "CHD4 (CC-AH-D)", "sequence": "MTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQ\r\nVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKW\r\nRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLD\r\nATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEM\r\nWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIV\r\nDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHD\r\nMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYL\r\nFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGI\r\nTGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVT\r\nRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDK\r\nAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQED\r\nLARNLGKGKRIRKQVNYNDGSQEDRAENLYFQ", "organism": "Homo sapiens", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 100.8, "total_mw": 100.8, "number_molecules": 1, "complex_state": null, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "50 mM HEPES 50 mM KCl", "concentration_unit": "mM", "comment": "4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid", "additive": "KCl 50.000 mM", "concentration": 50.0, "pka": 7.0, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "CHD4 (CC-AH-D)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Haydyn", "contributor_surname": "Mertens", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2010-11-13", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 15.0, "number_of_frames": 8, "wavelength": 0.15, "sample_detector_distance": 2.7, "concentration_min": 1.0, "concentration_max": 7.1, "sample_volume": null, "flow_rate": null, "s_min": 0.157, "s_max": 6.016, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDA95_fit1_model1_img.png", "software": "DAMMIF", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDA95_fit1_model1.pdb", "model_mw": 127.0, "bead_radius": 2.9, "log": "https://www.sasbdb.org/media/log_files/f10_3.log", "symmetry": "", "comment": "", "user": 12 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA95_fit1_fit_img.png", "software": null, "chi_square_value": 2.337841, "p_value": 0.000152, "fit_residual_plot": "SASDA95_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDA95_fit1.fir", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": "The ATPase CHD4 mediates nucleosome remodeling by the NuRD (nucleosome remodeling and deacetylase) complex. The NuRD complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The three dimensional small-angle X-ray scattering model of CHD4 helps to define its interdomain interactions, with cross linking and limited proteolysis studies used to validate the model. Functional and binding assays suggest a regulatory role for the PHD and chromo domains.", "experiment_description": null, "tags": [ "X33" ], "intensity_unit": null, "experimental_mw": 77.0, "experimental_mw_error": null, "guinier_i0_mw": 77.0, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 71.4, "guinier_i0_error": null, "pddf_rg": 4.0, "pddf_rg_error": null, "guinier_rg": 4.0, "guinier_rg_error": null, "pddf_dmax": 14.5, "pddf_dmax_error": null, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": null, "guinier_point_last": null, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDA95_datloglog_img.png", "symmetry": null, "last_modified": "2023-05-25T11:21:52.654103+02:00", "bragg_peak": [] }