{ "code": "SASDC97", "status": "Published", "type_of_curve": "Extrapolated to infinite dilution", "angular_unit": "1/nm", "project": { "title": "Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).", "publication": { "title": "Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).", "author_list": "Mayerhofer H, Panneerselvam S, Kaljunen H, Tuukkanen A, Mertens HD, Mueller-Dieckmann J", "journal": "J Biol Chem", "doi": "10.1074/jbc.M114.587667", "pmid": "25451923", "published_date": "2015 Jan 30" }, "status": "released", "submitted_date": "2017-02-07", "released_date": "2017-02-09" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDC97.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC97.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC97_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC97_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC97_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC97_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDC97.sascif", "experiment": { "instrument": { "detector": { "type": "Custom-built", "name": "Pilatus 1M-W", "resolution": 0.172 }, "name": "DORIS III, DESY", "city": "Hamburg", "country": "Germany", "beamline_name": "EMBL X33", "beam_geometry": "0.60", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains", "short_name": "ETR1_DHp-CA-RD", "sequence": "TAIRARNDFLAVMNHEMRTPMHAIIALSS\r\nLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLHTLFREVLNLIKPIAVVK\r\nKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSISVTALVTKSDTRAADFFVVPTGSHFYL\r\nRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRSSGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIF\r\nDVKLGISERSNESKQSGIPKVPAIPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLR\r\nVVSHEHKVVFMDVCMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVS\r\nLDNIRDVLSDLLEPRVLYEGM", "organism": "Arabidopsis thaliana", "uniprot_code": "P49333", "uniprot_range_first": 339, "uniprot_range_last": 738, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MEVCNCIEPQWPADELLMKYQYISDFFIAIAYFSIPLELIYFVKKSAVFPYRWVLVQFGA\nFIVLCGATHLINLWTFTTHSRTVALVMTTAKVLTAVVSCATALMLVHIIPDLLSVKTREL\nFLKNKAAELDREMGLIRTQEETGRHVRMLTHEIRSTLDRHTILKTTLVELGRTLALEECA\nLWMPTRTGLELQLSYTLRHQHPVEYTVPIQLPVINQVFGTSRAVKISPNSPVARLRPVSG\nKYMLGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVA\nDQVAVALSHAAILEESMRARDLLMEQNVALDLARREAETAIRARNDFLAVMNHEMRTPMH\nAIIALSSLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLH\nTLFREVLNLIKPIAVVKKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSI\nSVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRS\nSGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKLGISERSNESKQSGIPKVPA\nIPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLRVVSHEHKVVFMDV\nCMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVSLDN\nIRDVLSDLLEPRVLYEGM", "mw": 44.085, "total_mw": 88.17, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.8, "deuteration": null }, "purity_method": null, "name": "Ethylene Receptor 1 (DHp + CA + RD domains)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": 53494, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Haydyn", "contributor_surname": "Mertens", "orcid": null } ], "concentration_method": "A280", "concentration_unit": null, "date": "2010-10-11", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 15.0, "number_of_frames": 8, "wavelength": 0.154, "sample_detector_distance": 3.1, "concentration_min": 0.7, "concentration_max": 5.0, "sample_volume": null, "flow_rate": null, "s_min": 0.138, "s_max": 6.016, "total_exposure_time": 120.0, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "2.9.0", "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [ "X33" ], "intensity_unit": "arbitrary", "experimental_mw": 90.1, "experimental_mw_error": 24.0, "guinier_i0_mw": 66.4, "guinier_i0_mw_error": 5.0, "porod_mw": 90.1, "porod_mw_error": 24.0, "pddf_i0": 63.6999999997, "pddf_i0_error": 5.0, "guinier_i0": 68.19, "guinier_i0_error": 1.0, "pddf_rg": 4.1, "pddf_rg_error": 0.1, "guinier_rg": 3.98, "guinier_rg_error": 0.1, "pddf_dmax": 13.9, "pddf_dmax_error": 0.5, "porod_volume": 144.0, "porod_volume_error": 4.0, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 71, "pddf_point_first": 30, "pddf_point_last": 727, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDC97_datloglog_img.png", "symmetry": null, "last_modified": "2023-05-25T11:21:52.654103+02:00", "bragg_peak": [] }