data_SASDF98_MAIN # _sas_result.id 1480 _sas_result.SASBDB_code SASDF98 _sas_result.experimental_MW 23.000 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 36.000 _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 4905.0000000000 _sas_result.I0_from_Guinier_error 49.0000000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 4.290 _sas_result.Rg_from_Guinier_error 0.600 _sas_result.D_max 19.50 _sas_result.Porod_volume 58.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 21 _sas_result.Guinier_point_max 139 _sas_result.type_of_curve extrapolated _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments "There is a concentration effect in the concentration series for the sample in 10 mM salt buffer, so we use extrapolation to infinite dilution." # _sas_scan.title "Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl" _sas_scan.detc_id 4 _sas_scan.id 1480 _sas_scan.measurement_date 2016-10-03 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 20.0 _sas_scan.exposure_time 0.050 _sas_scan.number_of_frames 20 _sas_scan.unit 1/nm _sas_scan.qmin 0.0134 _sas_scan.qmax 3.7925 _sas_scan.sample_id 1441 _sas_scan.result_id 1480 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.00000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1441 _sas_sample.name "Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 0.80-2.60 _sas_sample.concentration_method . _sas_sample.entity_id 1738 # _sas_buffer.id 897 _sas_buffer.name "20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 6.600 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1441 # _entity.id 1738 _entity.type protein _entity.pdbx_description "DNA-directed RNA polymerase subunit delta - mutant" _entity.formula_weight 20.405 _entity.pdbx_number_of_molecules 1 # _entity_name_com.entity_id 1738 _entity_name_com.name "rpoE KE mutant" # _entity_poly.entity_id 1738 _entity_poly.pdbx_seq_one_letter_code MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVEAEEEEAEEAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK # _entity_src_gen.entity_id 1738 _entity_src_gen.gene_src_common_name "Bacillus subtilis (strain 168)" # _struct_ref.id 1 _struct_ref.entity_id 1738 _struct_ref.db_name UniProt _struct_ref.db_code P12464 _struct_ref.details http://www.uniprot.org/uniprot/P12464 # _citation.id 495 _citation.title "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase." _citation.year "2019 Sep 24" _citation.pdbx_database_id_DOI 10.1021/jacs.9b07837 _citation.pdbx_database_id_PubMed 31550880 # loop_ _citation_author.citation_id _citation_author.ordinal _citation_author.name 495 1 "Kuban V" 495 2 "Srb P" 495 3 "Stegnerova H" 495 4 "Padrta P" 495 5 "Zachrdla M" 495 6 "Jasenakova Z" 495 7 "Šanderová H" 495 8 "Vítovská D" 495 9 "Krasny L" 495 10 "Koval T" 495 11 "Dohnalek J" 495 12 "Ziemska-Legi Cka J" 495 13 "Grynberg M" 495 14 "Jarnot P" 495 15 "Gruca A" 495 16 "Jensen MR" 495 17 "Blackledge M" # _pdbx_contact_author.id 83 _pdbx_contact_author.name_first Vojtěch _pdbx_contact_author.name_last Kubáň _pdbx_contact_author.role . _pdbx_contact_author.address_1 MUNI _pdbx_contact_author.address_2 "Masaryk University; Research group Structure and Dynamics of Proteins." _pdbx_contact_author.address_3 "Brno, Czech Republic" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 1.337960E-02 -7.308551E+03 2.441808E+04 1480 2 1.546980E-02 -1.157187E+03 1.557137E+04 1480 3 1.756010E-02 6.516238E+03 9.733100E+03 1480 4 1.965030E-02 3.318746E+03 6.591580E+03 1480 5 2.174050E-02 7.299416E+03 5.367837E+03 1480 6 2.383070E-02 9.401733E+03 4.195377E+03 1480 7 2.592090E-02 6.545583E+03 3.735578E+03 1480 8 2.801120E-02 9.873189E+03 2.883179E+03 1480 9 3.010140E-02 9.137157E+03 2.500700E+03 1480 10 3.219160E-02 5.013224E+03 2.163307E+03 1480 11 3.428180E-02 6.114102E+03 1.813476E+03 1480 12 3.637210E-02 3.632211E+03 1.655366E+03 1480 13 3.846230E-02 4.440803E+03 1.364740E+03 1480 14 4.055250E-02 3.490831E+03 1.247826E+03 1480 15 4.264270E-02 5.447142E+03 1.144307E+03 1480 16 4.473290E-02 5.244051E+03 1.007738E+03 1480 17 4.682320E-02 5.396037E+03 9.535025E+02 1480 18 4.891340E-02 5.293772E+03 8.753430E+02 1480 19 5.100360E-02 5.637469E+03 8.717264E+02 1480 20 5.309380E-02 5.587527E+03 7.935097E+02 1480 21 5.518410E-02 5.195245E+03 7.385020E+02 1480 22 5.727430E-02 4.905977E+03 7.155172E+02 1480 23 5.936450E-02 5.929422E+03 6.624227E+02 1480 24 6.145470E-02 4.470342E+03 6.656614E+02 1480 25 6.354490E-02 5.126619E+03 5.960687E+02 1480 26 6.563520E-02 5.188037E+03 5.854188E+02 1480 27 6.772540E-02 5.497798E+03 5.634998E+02 1480 28 6.981560E-02 4.750523E+03 5.477505E+02 1480 29 7.190580E-02 4.426355E+03 4.992333E+02 1480 30 7.399610E-02 4.115593E+03 4.833790E+02 1480 31 7.608630E-02 5.246513E+03 4.870148E+02 1480 32 7.817650E-02 4.952469E+03 4.658067E+02 1480 33 8.026670E-02 5.065051E+03 4.510603E+02 1480 34 8.235690E-02 5.156665E+03 4.342412E+02 1480 35 8.444720E-02 5.211947E+03 4.248254E+02 1480 36 8.653740E-02 4.988007E+03 4.189223E+02 1480 37 8.862760E-02 4.590244E+03 3.921985E+02 1480 38 9.071780E-02 4.482837E+03 3.887012E+02 1480 39 9.280810E-02 4.881557E+03 3.786976E+02 1480 40 9.489830E-02 5.737124E+03 3.855197E+02 1480 41 9.698850E-02 5.293429E+03 3.810045E+02 1480 42 9.907870E-02 4.840264E+03 3.540116E+02 1480 43 1.011690E-01 4.879394E+03 3.585982E+02 1480 44 1.032590E-01 5.181055E+03 3.474953E+02 1480 45 1.053490E-01 4.541961E+03 3.506605E+02 1480 46 1.074400E-01 4.472593E+03 3.317324E+02 1480 47 1.095300E-01 4.661087E+03 3.264302E+02 1480 48 1.116200E-01 4.941138E+03 3.426416E+02 1480 49 1.137100E-01 4.529444E+03 3.203704E+02 1480 50 1.158000E-01 4.927389E+03 3.170170E+02 1480 51 1.178910E-01 4.667357E+03 3.117804E+02 1480 52 1.199810E-01 3.785816E+03 3.001191E+02 1480 53 1.220710E-01 4.940060E+03 3.140061E+02 1480 54 1.241610E-01 4.919486E+03 2.932633E+02 1480 55 1.262520E-01 4.304128E+03 2.921218E+02 1480 56 1.283420E-01 4.643594E+03 2.938113E+02 1480 57 1.304320E-01 4.174949E+03 2.840316E+02 1480 58 1.325220E-01 4.856992E+03 2.913604E+02 1480 59 1.346120E-01 5.172184E+03 2.869731E+02 1480 60 1.367030E-01 4.353154E+03 2.807799E+02 1480 61 1.387930E-01 4.465395E+03 2.743296E+02 1480 62 1.408830E-01 4.248071E+03 2.747370E+02 1480 63 1.429730E-01 4.327161E+03 2.636706E+02 1480 64 1.450640E-01 4.426719E+03 2.668588E+02 1480 65 1.471540E-01 3.821994E+03 2.635977E+02 1480 66 1.492440E-01 3.907395E+03 2.575815E+02 1480 67 1.513340E-01 3.747830E+03 2.546466E+02 1480 68 1.534240E-01 4.276308E+03 2.529314E+02 1480 69 1.555150E-01 4.620466E+03 2.582153E+02 1480 70 1.576050E-01 4.485209E+03 2.580632E+02 1480 71 1.596950E-01 4.293799E+03 2.477839E+02 1480 72 1.617850E-01 4.391787E+03 2.481693E+02 1480 73 1.638760E-01 4.115133E+03 1.354603E+02 1480 74 1.659660E-01 3.697670E+03 1.361377E+02 1480 75 1.680560E-01 4.289499E+03 1.360840E+02 1480 76 1.701460E-01 3.895408E+03 1.308783E+02 1480 77 1.722360E-01 4.090888E+03 1.347728E+02 1480 78 1.743270E-01 4.091400E+03 1.304171E+02 1480 79 1.764170E-01 4.087791E+03 1.305347E+02 1480 80 1.785070E-01 3.929047E+03 1.244839E+02 1480 81 1.805970E-01 4.066643E+03 1.294140E+02 1480 82 1.826880E-01 3.968046E+03 1.290443E+02 1480 83 1.847780E-01 3.930394E+03 1.253532E+02 1480 84 1.868680E-01 3.731678E+03 1.236560E+02 1480 85 1.889580E-01 3.608294E+03 1.221280E+02 1480 86 1.910480E-01 3.829514E+03 1.225156E+02 1480 87 1.931390E-01 3.765506E+03 1.243332E+02 1480 88 1.952290E-01 3.889298E+03 1.194387E+02 1480 89 1.973190E-01 3.812448E+03 1.232090E+02 1480 90 1.994090E-01 3.715176E+03 1.176559E+02 1480 91 2.015000E-01 3.497528E+03 1.184832E+02 1480 92 2.035900E-01 3.606993E+03 1.164766E+02 1480 93 2.056800E-01 3.831789E+03 1.157500E+02 1480 94 2.077700E-01 3.599776E+03 1.184019E+02 1480 95 2.098600E-01 3.731214E+03 1.154249E+02 1480 96 2.119510E-01 3.713989E+03 1.181213E+02 1480 97 2.140410E-01 3.665873E+03 1.125853E+02 1480 98 2.161310E-01 3.650422E+03 1.129319E+02 1480 99 2.182210E-01 3.729152E+03 1.162366E+02 1480 100 2.203120E-01 3.641550E+03 1.122216E+02 1480 101 2.224020E-01 3.797324E+03 1.113442E+02 1480 102 2.244920E-01 3.384775E+03 1.108683E+02 1480 103 2.265820E-01 3.722444E+03 1.114477E+02 1480 104 2.286720E-01 3.578917E+03 1.098055E+02 1480 105 2.307630E-01 3.335828E+03 1.078938E+02 1480 106 2.328530E-01 3.685536E+03 1.095553E+02 1480 107 2.349430E-01 3.421900E+03 1.082087E+02 1480 108 2.370330E-01 3.328874E+03 1.090386E+02 1480 109 2.391240E-01 3.408842E+03 1.073814E+02 1480 110 2.412140E-01 3.342012E+03 1.049742E+02 1480 111 2.433040E-01 3.393084E+03 1.071111E+02 1480 112 2.453940E-01 3.335154E+03 1.052635E+02 1480 113 2.474840E-01 3.420814E+03 1.065698E+02 1480 114 2.495750E-01 3.419110E+03 1.023309E+02 1480 115 2.516650E-01 3.061118E+03 1.037954E+02 1480 116 2.537550E-01 3.609995E+03 1.040285E+02 1480 117 2.558450E-01 3.520318E+03 1.039630E+02 1480 118 2.579360E-01 3.302482E+03 1.017260E+02 1480 119 2.600260E-01 3.070396E+03 1.009110E+02 1480 120 2.621160E-01 3.312563E+03 1.018207E+02 1480 121 2.642060E-01 3.108060E+03 1.004293E+02 1480 122 2.662960E-01 3.112992E+03 9.909886E+01 1480 123 2.683870E-01 3.051482E+03 9.912139E+01 1480 124 2.704770E-01 3.150200E+03 9.833753E+01 1480 125 2.725670E-01 3.099936E+03 9.963954E+01 1480 126 2.746570E-01 3.068529E+03 9.726184E+01 1480 127 2.767480E-01 3.027641E+03 9.728162E+01 1480 128 2.788380E-01 3.012127E+03 9.713434E+01 1480 129 2.809280E-01 3.135219E+03 9.677671E+01 1480 130 2.830180E-01 2.957326E+03 9.678076E+01 1480 131 2.851080E-01 3.057360E+03 9.443639E+01 1480 132 2.871990E-01 3.050671E+03 9.769483E+01 1480 133 2.892890E-01 3.068708E+03 9.525420E+01 1480 134 2.913790E-01 2.982946E+03 9.420149E+01 1480 135 2.934690E-01 2.955643E+03 9.412017E+01 1480 136 2.955600E-01 3.085098E+03 9.277804E+01 1480 137 2.976500E-01 2.897085E+03 9.432885E+01 1480 138 2.997400E-01 2.816626E+03 9.248093E+01 1480 139 3.018300E-01 2.724267E+03 9.108230E+01 1480 140 3.039200E-01 2.680052E+03 9.196814E+01 1480 141 3.060110E-01 2.891032E+03 9.159576E+01 1480 142 3.081010E-01 2.826341E+03 9.437066E+01 1480 143 3.101910E-01 2.818416E+03 8.942030E+01 1480 144 3.122810E-01 2.763453E+03 8.974254E+01 1480 145 3.143720E-01 2.672609E+03 9.092753E+01 1480 146 3.164620E-01 2.650309E+03 8.952570E+01 1480 147 3.185520E-01 2.710040E+03 8.979894E+01 1480 148 3.206420E-01 2.667043E+03 8.669242E+01 1480 149 3.227320E-01 2.626982E+03 8.958264E+01 1480 150 3.248230E-01 2.663268E+03 8.876016E+01 1480 151 3.269130E-01 2.687532E+03 7.760859E+01 1480 152 3.290030E-01 2.896339E+03 8.091773E+01 1480 153 3.310930E-01 2.639943E+03 7.528897E+01 1480 154 3.331840E-01 2.617491E+03 7.764518E+01 1480 155 3.352740E-01 2.583566E+03 7.632077E+01 1480 156 3.373640E-01 2.523423E+03 7.510222E+01 1480 157 3.394540E-01 2.469072E+03 7.556878E+01 1480 158 3.415440E-01 2.429538E+03 7.428530E+01 1480 159 3.436350E-01 2.448240E+03 7.519899E+01 1480 160 3.457250E-01 2.415117E+03 7.391927E+01 1480 161 3.478150E-01 2.529767E+03 7.526085E+01 1480 162 3.499050E-01 2.442754E+03 7.754469E+01 1480 163 3.519960E-01 2.360813E+03 7.874183E+01 1480 164 3.540860E-01 2.483613E+03 8.154445E+01 1480 165 3.561760E-01 2.566337E+03 7.926660E+01 1480 166 3.582660E-01 2.305885E+03 8.186756E+01 1480 167 3.603560E-01 2.280886E+03 8.174098E+01 1480 168 3.624470E-01 2.312100E+03 8.405921E+01 1480 169 3.645370E-01 2.437136E+03 8.383993E+01 1480 170 3.666270E-01 2.443462E+03 8.430374E+01 1480 171 3.687170E-01 2.403134E+03 8.605123E+01 1480 172 3.708080E-01 2.545830E+03 8.586781E+01 1480 173 3.728980E-01 2.246194E+03 8.334674E+01 1480 174 3.749880E-01 2.311596E+03 8.476453E+01 1480 175 3.770780E-01 2.183988E+03 8.541283E+01 1480 176 3.791680E-01 2.141527E+03 8.492017E+01 1480 177 3.812590E-01 2.217579E+03 8.145506E+01 1480 178 3.833490E-01 2.254852E+03 8.589682E+01 1480 179 3.854390E-01 2.123278E+03 8.413989E+01 1480 180 3.875290E-01 2.142343E+03 8.418997E+01 1480 181 3.896200E-01 2.157372E+03 8.588367E+01 1480 182 3.917100E-01 2.264349E+03 8.268887E+01 1480 183 3.938000E-01 2.263642E+03 8.359515E+01 1480 184 3.958900E-01 2.040985E+03 7.807806E+01 1480 185 3.979800E-01 2.168147E+03 7.824984E+01 1480 186 4.000710E-01 2.221434E+03 7.814505E+01 1480 187 4.021610E-01 2.066923E+03 7.539341E+01 1480 188 4.042510E-01 1.998809E+03 7.741124E+01 1480 189 4.063410E-01 2.233436E+03 7.508393E+01 1480 190 4.084320E-01 2.177530E+03 7.564278E+01 1480 191 4.105220E-01 2.129753E+03 7.455404E+01 1480 192 4.126120E-01 2.140737E+03 7.299899E+01 1480 193 4.147020E-01 1.973692E+03 7.461494E+01 1480 194 4.167920E-01 2.034120E+03 7.203374E+01 1480 195 4.188830E-01 2.167609E+03 7.234591E+01 1480 196 4.209730E-01 2.109332E+03 7.246053E+01 1480 197 4.230630E-01 2.133932E+03 7.211829E+01 1480 198 4.251530E-01 1.978837E+03 7.112602E+01 1480 199 4.272440E-01 2.022493E+03 7.053146E+01 1480 200 4.293340E-01 2.021777E+03 7.102837E+01 1480 201 4.314240E-01 1.851406E+03 6.947004E+01 1480 202 4.335140E-01 1.923807E+03 7.029754E+01 1480 203 4.356040E-01 1.811298E+03 6.868882E+01 1480 204 4.376950E-01 2.027070E+03 6.948374E+01 1480 205 4.397850E-01 2.049643E+03 7.028517E+01 1480 206 4.418750E-01 1.882905E+03 6.847029E+01 1480 207 4.439650E-01 1.773693E+03 6.765520E+01 1480 208 4.460560E-01 1.907122E+03 6.788413E+01 1480 209 4.481460E-01 2.038084E+03 6.815022E+01 1480 210 4.502360E-01 1.964773E+03 6.897114E+01 1480 211 4.523260E-01 1.886481E+03 6.599407E+01 1480 212 4.544160E-01 2.010874E+03 6.720787E+01 1480 213 4.565070E-01 1.892649E+03 6.715251E+01 1480 214 4.585970E-01 1.874780E+03 6.656807E+01 1480 215 4.606870E-01 1.915498E+03 6.577085E+01 1480 216 4.627770E-01 1.906829E+03 6.561550E+01 1480 217 4.648680E-01 1.965335E+03 6.615810E+01 1480 218 4.669580E-01 1.912832E+03 6.546436E+01 1480 219 4.690480E-01 1.846018E+03 6.586986E+01 1480 220 4.711380E-01 1.813505E+03 6.413641E+01 1480 221 4.732280E-01 1.792299E+03 6.488681E+01 1480 222 4.753190E-01 1.856786E+03 6.498388E+01 1480 223 4.774090E-01 1.779344E+03 6.495314E+01 1480 224 4.794990E-01 1.841355E+03 6.347725E+01 1480 225 4.815890E-01 1.777776E+03 6.334879E+01 1480 226 4.836800E-01 1.764576E+03 6.441305E+01 1480 227 4.857700E-01 1.771295E+03 6.298285E+01 1480 228 4.878600E-01 1.809339E+03 6.302903E+01 1480 229 4.899500E-01 1.679702E+03 6.281732E+01 1480 230 4.920400E-01 1.836595E+03 6.265778E+01 1480 231 4.941310E-01 1.721839E+03 6.314729E+01 1480 232 4.962210E-01 1.716591E+03 6.211580E+01 1480 233 4.983110E-01 1.780447E+03 6.142378E+01 1480 234 5.004010E-01 1.844427E+03 6.211221E+01 1480 235 5.024920E-01 1.627151E+03 6.089000E+01 1480 236 5.045820E-01 1.725926E+03 6.166817E+01 1480 237 5.066720E-01 1.716356E+03 6.043768E+01 1480 238 5.087620E-01 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